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Albert Blanchart, head manager ViruTech Facility KI

Albert Blanchart Aguado

Laboratory coordinator

About me

I received my PhD in developmental biology from Complutense University (Madrid, Spain) in the Cajal Institute (CSIC, Spain), working on olfactory development and the contribution from the peripheral nervous system (the olfactory epithelium) to the olfactory bulb development, as well as the role of the anterior subventricular zone in the neuronal pool maintenance in the olfactory bulb. I then move to Sweden and started a post doc within Patrik Ernfors Lab in the department of Medical Biochemistry and Biophysics, working in brain cancer and stem cells, and the role of epigenetic factors in the maintenance and proliferation of stem cells.

During my years of research, I became familiarized with a broad range of techniques used in research. My research projects required master of a broad spectrum of different biological and biochemical techniques, including animal handling, surgery and colony management of different transgenic mouse lines; cell culture and virus production working in BSL1 and BSL2 labs; immunochemistry; molecular biology and cellular biology.

As a post doc I managed the virus facility in the department of Medical Biochemistry and Biophysics, as I was one of the main users. I have experience working in BSL2 labs and with the biosafety regulations and rules applied to them.

Publications:

UHRF1 Licensed Self-Renewal of Active Adult Neural Stem Cells. Blanchart A, Navis AC, Assaife-Lopes N, Usoskin D, Aranda S, Sontheimer J, Ernfors P. Stem Cells. 2018 Nov;36(11):1736-1751. doi: 10.1002/stem.2889. Epub 2018 Aug 18. PMID:29999568

Endogenous GABAAreceptor activity suppresses glioma growth. Blanchart A, Fernando R, Häring M, Assaife-Lopes N, Romanov RA, Andäng M, Harkany T, Ernfors P. Oncogene. 2017 Feb 9;36(6):777-786. doi: 10.1038/onc.2016.245. Epub 2016 Jul 4.PMID: 27375015

Sox2 and Mitf cross-regulatory interactions consolidate progenitor and melanocyte lineages in the cranial neural crest. Adameyko I, Lallemend F, Furlan A, Zinin N, Aranda S, Kitambi SS, Blanchart A, Favaro R, Nicolis S, Lübke M, Müller T, Birchmeier C, Suter U, Zaitoun I, Takahashi Y, Ernfors P. Development. 2012 Jan;139(2):397-410. doi: 10.1242/dev.065581. PMID:22186729

Dab1 (Disable homolog-1) reelin adaptor protein is overexpressed in the olfactory bulb at early postnatal stages. Martín-López E, Blanchart A, De Carlos JA, López-Mascaraque L. PLoS One. 2011;6(10):e26673. doi: 10.1371/journal.pone.0026673. Epub 2011 Oct 25.PMID:22046330

Synaptogenesis in the mouse olfactory bulb during glomerulus development. Blanchart A, Romaguera M, García-Verdugo JM, de Carlos JA, López-Mascaraque L. Eur J Neurosci. 2008 Jun;27(11):2838-46. doi: 10.1111/j.1460-9568.2008.06283.x.

Time frame of mitral cell development in the mice olfactory bulb. Blanchart A, De Carlos JA, López-Mascaraque L. J Comp Neurol. 2006 Jun 1;496(4):529-43.

Olfactory epithelium influences the orientation of mitral cell dendrites during development. López-Mascaraque L, García C, Blanchart A, De Carlos JA. Dev Dyn. 2005 Feb;232(2):325-35.

Research description

The VirusTech Core Facility at Karolinska Institutet has, as a primary goal, to provide the most advanced viral vector technologies, helping researchers in their projects, by using these genetic tools to efficiently deliver genes into both in vitro and in vivo cells.

Our primary purpose is to produce, purify and concentrate viral particles and deliver them ready to use to our fellow resercher. We also aim to optimize and improve the current exixting protocols in order to offer an even faster and higher quality service.

 

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