Anna Pasetto

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About me

I joined the Karolinska Institutet in 2018 as assistant professor (biträdande universitetslektor) and managing director of the pre-GMP facility in the department of Laboratory Medicine. I received my Master Degree in Molecular Biotechnology in 2007 from Alma Mater Studiorum Bologna University, Italy. I then moved to the Karolinska Institutet where I received my PhD in 2012. I joined the laboratory of Dr. Steven Rosenberg at the National Cancer Institute, NIH in 2013 and my research was focused on the isolation of TCR genes from mutation-reactive tumor infiltrating lymphocytes in metastatic solid cancers.

Research

My research goal is to develop new immunotherapies against cancer. The main focus is to identify immunological receptors able to recognize mutated antigens expressed by cancer, these receptors can then be engineered to generate CAR-T cells, TCR-T cells and other types of cell therapies.

Articles

Article: MOLECULAR THERAPY. 2024;32(2):540-555

Distinct roles of vaccine-induced SARS-CoV-2-specific neutralizing antibodies and T cells in protection and disease.

Yan J; Bangalore CR; Nikouyan N; Appelberg S; Silva DN; Yao H; Pasetto A; Weber F; Weber S; Larsson O; Höglund U; Bogdanovic G; Grabbe M; Aleman S; Szekely L; Szakos A; Tuvesson O; Gidlund E-K; Cadossi M; Salati S; Tegel H; Hober S; Frelin L; Mirazimi A; Ahlén G; Sällberg M
Article: NPJ PRECISION ONCOLOGY. 2024;8(1):26

Autologous anti-GD2 CAR T cells efficiently target primary human glioblastoma.

Chiavelli C; Prapa M; Rovesti G; Silingardi M; Neri G; Pugliese G; Trudu L; Dall'Ora M; Golinelli G; Grisendi G; Vinet J; Bestagno M; Spano C; Papapietro RV; Depenni R; Di Emidio K; Pasetto A; Nascimento Silva D; Feletti A; Berlucchi S; Iaccarino C; Pavesi G; Dominici M
Article: GUT. 2023;72(6):1186-1195

Novel prime-boost immune-based therapy inhibiting both hepatitis B and D virus infections.

Burm R; Maravelia P; Ahlen G; Ciesek S; Caro Perez N; Pasetto A; Urban S; Van Houtte F; Verhoye L; Wedemeyer H; Johansson M; Frelin L; Sällberg M; Meuleman P
Article: CELL REPORTS. 2022;40(5):111153

Cancer genes disfavoring T cell immunity identified via integrated systems approach.

Kishton RJ; Patel SJ; Decker AE; Vodnala SK; Cam M; Yamamoto TN; Patel Y; Sukumar M; Yu Z; Ji M; Henning AN; Gurusamy D; Palmer DC; Stefanescu RA; Girvin AT; Lo W; Pasetto A; Malekzadeh P; Deniger DC; Wood KC; Sanjana NE; Restifo NP
Article: METHODS IN MOLECULAR BIOLOGY. 2022;2574:209-219

T-Cell Repertoire Characterization.

Pasetto A; Buggert M
Article: FRONTIERS IN IMMUNOLOGY. 2022;13:896242

Process Development for Adoptive Cell Therapy in Academia: A Pipeline for Clinical-Scale Manufacturing of Multiple TCR-T Cell Products.

Silva DN; Chrobok M; Rovesti G; Healy K; Wagner AK; Maravelia P; Gatto F; Mazza M; Mazzotti L; Lohmann V; Sällberg Chen M; Sällberg M; Buggert M; Pasetto A
Article: JOURNAL OF INFECTIOUS DISEASES. 2021;223(1):128-138

Blocking Entry of Hepatitis B and D Viruses to Hepatocytes as a Novel Immunotherapy for Treating Chronic Infections.

Maravelia P; Frelin L; Ni Y; Caro Pérez N; Ahlén G; Jagya N; Verch G; Verhoye L; Pater L; Johansson M; Pasetto A; Meuleman P; Urban S; Sällberg M
Article: JOURNAL OF IMMUNOTHERAPY. 2021;44(1):1-8

Rapid Identification and Evaluation of Neoantigen-reactive T-Cell Receptors From Single Cells.

Paria BC; Levin N; Lowery FJ; Pasetto A; Deniger DC; Parkhurst MR; Yossef R; Kim SP; Florentin M; Ngo LT; Ray S; Krishna S; Robbins PF; Rosenberg SA
Article: JOURNAL OF CLINICAL INVESTIGATION. 2019;129(11):4992-5004

Recognition of human gastrointestinal cancer neoantigens by circulating PD-1+ lymphocytes.

Gros A; Tran E; Parkhurst MR; Ilyas S; Pasetto A; Groh EM; Robbins PF; Yossef R; Garcia-Garijo A; Fajardo CA; Prickett TD; Jia L; Gartner JJ; Ray S; Ngo L; Wunderllich JR; Yang JC; Rosenberg SA
Article: CANCER DISCOVERY. 2019;9(8):1022-1035

Unique Neoantigens Arise from Somatic Mutations in Patients with Gastrointestinal Cancers.

Parkhurst MR; Robbins PF; Tran E; Prickett TD; Gartner JJ; Jia L; Ivey G; Li YF; El-Gamil M; Lalani A; Crystal JS; Sachs A; Groh E; Ray S; Ngo LT; Kivitz S; Pasetto A; Yossef R; Lowery FJ; Goff SL; Lo W; Cafri G; Deniger DC; Malekzadeh P; Ahmadzadeh M; Wunderlich JR; Somerville RPT; Rosenberg SA
Article: CANCER IMMUNOLOGY RESEARCH. 2019;7(4):534-543

Immunologic Recognition of a Shared p53 Mutated Neoantigen in a Patient with Metastatic Colorectal Cancer.

Lo W; Parkhurst M; Robbins PF; Tran E; Lu Y-C; Jia L; Gartner JJ; Pasetto A; Deniger D; Malekzadeh P; Shelton TE; Prickett T; Ray S; Kivitz S; Paria BC; Kriley I; Schrump DS; Rosenberg SA
Article: JOURNAL OF CLINICAL INVESTIGATION. 2019;129(3):1109-1114

Neoantigen screening identifies broad TP53 mutant immunogenicity in patients with epithelial cancers.

Malekzadeh P; Pasetto A; Robbins PF; Parkhurst MR; Paria BC; Jia L; Gartner JJ; Hill V; Yu Z; Restifo NP; Sachs A; Tran E; Lo W; Somerville RP; Rosenberg SA; Deniger DC
Article: NATURE COMMUNICATIONS. 2019;10(1):449

Memory T cells targeting oncogenic mutations detected in peripheral blood of epithelial cancer patients.

Cafri G; Yossef R; Pasetto A; Deniger DC; Lu Y-C; Parkhurst M; Gartner JJ; Jia L; Ray S; Ngo LT; Jafferji M; Sachs A; Prickett T; Robbins PF; Rosenberg SA
Article: SCIENCE IMMUNOLOGY. 2019;4(31):eaao4310

Tumor-infiltrating human CD4+ regulatory T cells display a distinct TCR repertoire and exhibit tumor and neoantigen reactivity.

Ahmadzadeh M; Pasetto A; Jia L; Deniger DC; Stevanović S; Robbins PF; Rosenberg SA
Article: CLINICAL CANCER RESEARCH. 2018;24(22):5562-5573

T-cell Responses to TP53 "Hotspot" Mutations and Unique Neoantigens Expressed by Human Ovarian Cancers.

Deniger DC; Pasetto A; Robbins PF; Gartner JJ; Prickett TD; Paria BC; Malekzadeh P; Jia L; Yossef R; Langhan MM; Wunderlich JR; Danforth DN; Somerville RPT; Rosenberg SA
Article: JCI INSIGHT. 2018;3(19):122467

Enhanced detection of neoantigen-reactive T cells targeting unique and shared oncogenes for personalized cancer immunotherapy.

Yossef R; Tran E; Deniger DC; Gros A; Pasetto A; Parkhurst MR; Gartner JJ; Prickett TD; Cafri G; Robbins PF; Rosenberg SA
Article: NATURE MEDICINE. 2018;24(6):724-730

Immune recognition of somatic mutations leading to complete durable regression in metastatic breast cancer.

Zacharakis N; Chinnasamy H; Black M; Xu H; Lu Y-C; Zheng Z; Pasetto A; Langhan M; Shelton T; Prickett T; Gartner J; Jia L; Trebska-McGowan K; Somerville RP; Robbins PF; Rosenberg SA; Goff SL; Feldman SA
Article: CLINICAL CANCER RESEARCH. 2017;23(10):2491-2505

Isolation of T-Cell Receptors Specifically Reactive with Mutated Tumor-Associated Antigens from Tumor-Infiltrating Lymphocytes Based on CD137 Expression.

Parkhurst M; Gros A; Pasetto A; Prickett T; Crystal JS; Robbins P; Rosenberg SA
Article: JOURNAL OF VIROLOGY. 2017;91(9):e00010-e00017

Hepatitis C Virus-Specific T Cell Receptor mRNA-Engineered Human T Cells: Impact of Antigen Specificity on Functional Properties.

Balasiddaiah A; Davanian H; Aleman S; Pasetto A; Frelin L; Sällberg M; Lohmann V; Koh S; Bertoletti A; Chen M
Article: SCIENCE. 2017;356(6334):200-205

Landscape of immunogenic tumor antigens in successful immunotherapy of virally induced epithelial cancer.

Stevanović S; Pasetto A; Helman SR; Gartner JJ; Prickett TD; Howie B; Robins HS; Robbins PF; Klebanoff CA; Rosenberg SA; Hinrichs CS
Article: CLINICAL CANCER RESEARCH. 2017;23(2):351-362

A Pilot Trial of the Combination of Vemurafenib with Adoptive Cell Therapy in Patients with Metastatic Melanoma.

Deniger DC; Kwong MLM; Pasetto A; Dudley ME; Wunderlich JR; Langhan MM; Lee C-CR; Rosenberg SA
Article: NEW ENGLAND JOURNAL OF MEDICINE. 2016;375(23):2255-2262

T-Cell Transfer Therapy Targeting Mutant KRAS in Cancer.

Tran E; Robbins PF; Lu Y-C; Prickett TD; Gartner JJ; Jia L; Pasetto A; Zheng Z; Ray S; Groh EM; Kriley IR; Rosenberg SA
Article: CANCER IMMUNOLOGY RESEARCH. 2016;4(9):734-743

Tumor- and Neoantigen-Reactive T-cell Receptors Can Be Identified Based on Their Frequency in Fresh Tumor.

Pasetto A; Gros A; Robbins PF; Deniger DC; Prickett TD; Matus-Nicodemos R; Douek DC; Howie B; Robins H; Parkhurst MR; Gartner J; Trebska-McGowan K; Crystal JS; Rosenberg SA
Article: CANCER IMMUNOLOGY RESEARCH. 2016;4(8):669-678

Durable Complete Response from Metastatic Melanoma after Transfer of Autologous T Cells Recognizing 10 Mutated Tumor Antigens.

Prickett TD; Crystal JS; Cohen CJ; Pasetto A; Parkhurst MR; Gartner JJ; Yao X; Wang R; Gros A; Li YF; El-Gamil M; Trebska-McGowan K; Rosenberg SA; Robbins PF
Article: MOLECULAR THERAPY. 2016;24(6):1078-1089

Stable, Nonviral Expression of Mutated Tumor Neoantigen-specific T-cell Receptors Using the Sleeping Beauty Transposon/Transposase System.

Deniger DC; Pasetto A; Tran E; Parkhurst MR; Cohen CJ; Robbins PF; Cooper LJ; Rosenberg SA
Article: NATURE MEDICINE. 2016;22(4):433-438

Prospective identification of neoantigen-specific lymphocytes in the peripheral blood of melanoma patients.

Gros A; Parkhurst MR; Tran E; Pasetto A; Robbins PF; Ilyas S; Prickett TD; Gartner JJ; Crystal JS; Roberts IM; Trebska-McGowan K; Wunderlich JR; Yang JC; Rosenberg SA
Article: JOURNAL OF IMMUNOLOGY. 2013;190(3):1113-1124

A synthetic codon-optimized hepatitis C virus nonstructural 5A DNA vaccine primes polyfunctional CD8+ T cell responses in wild-type and NS5A-transgenic mice.

Holmström F; Pasetto A; Nähr V; Brass A; Kriegs M; Hildt E; Broderick KE; Chen M; Ahlén G; Frelin L
Article: PLOS ONE. 2013;8(6):e65964

The adjuvant activity of alphavirus replicons is enhanced by incorporating the microbial molecule flagellin into the replicon.

Knudsen ML; Johansson DX; Kostic L; Nordström EKL; Tegerstedt K; Pasetto A; Applequist SE; Ljungberg K; Sirard J-C; Liljeström P
Article: JOURNAL OF IMMUNOLOGY. 2012;189(9):4510-4519

TCR-redirected human T cells inhibit hepatitis C virus replication: hepatotoxic potential is linked to antigen specificity and functional avidity.

Pasetto A; Frelin L; Aleman S; Holmström F; Brass A; Ahlén G; Brenndörfer ED; Lohmann V; Bartenschlager R; Sällberg M; Bertoletti A; Chen M
Article: JOURNAL OF GENERAL VIROLOGY. 2012;93(Pt 2):247-258

Generation of T-cell receptors targeting a genetically stable and immunodominant cytotoxic T-lymphocyte epitope within hepatitis C virus non-structural protein 3.

Pasetto A; Frelin L; Brass A; Yasmeen A; Koh S; Lohmann V; Bartenschlager R; Magalhaes I; Maeurer M; Sällberg M; Chen M
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All other publications

Editorial comment: FRONTIERS IN ONCOLOGY. 2023;13:1171907

Editorial: Personalized immunotherapy for cancer.

Pasetto A; Lu Y-C
Review: IMMUNO-ONCOLOGY AND TECHNOLOGY. 2022;16:100099

ATMP development and pre-GMP environment in academia: a safety net for early cell and gene therapy development and manufacturing.

Silva DN; Chrobok M; Ahlén G; Blomberg P; Sällberg M; Pasetto A
Review: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2022;23(15):8590

T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer.

Mazzotti L; Gaimari A; Bravaccini S; Maltoni R; Cerchione C; Juan M; Navarro EA-G; Pasetto A; Nascimento Silva D; Ancarani V; Sambri V; Calabrò L; Martinelli G; Mazza M
Review: CANCERS. 2021;13(17):4334

Liquid Biopsy in Hepatocellular Carcinoma: Opportunities and Challenges for Immunotherapy.

Maravelia P; Silva DN; Rovesti G; Chrobok M; Stål P; Lu Y-C; Pasetto A
Review: FRONTIERS IN IMMUNOLOGY. 2021;12:689091

Single-Cell TCR and Transcriptome Analysis: An Indispensable Tool for Studying T-Cell Biology and Cancer Immunotherapy.

Pasetto A; Lu Y-C
Editorial comment: CANCERS. 2020;12(10):E2818-2818

Identification and Clinical Application of Immunological Receptors Targeting Mutated Antigens Expressed by Solid Tumors.

Pasetto A
Review: CELLS. 2020;9(6):E1471-1471

Chronic Viral Liver Diseases: Approaching the Liver Using T Cell Receptor-Mediated Gene Technologies.

Healy K; Pasetto A; Sobkowiak MJ; Soon CF; Cornberg M; Aleman S; Sällberg Chen M
Review: FRONTIERS IN IMMUNOLOGY. 2020;11:552

Liver, Tumor and Viral Hepatitis: Key Players in the Complex Balance Between Tolerance and Immune Activation.

Sällberg M; Pasetto A
Editorial comment: ALIMENTARY PHARMACOLOGY & THERAPEUTICS. 2019;50(8):963-964

Editorial: HBV-the naked truth?

Pasetto A; Ahlén G; Sällberg M
Review: ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS. 2014;62(1):23-30

Functional attributes of responding T cells in HCV infection: the recent advances in engineering functional antiviral T cells.

Pasetto A; Aleman S; Chen M

Employments

Research Specialist, Department of Laboratory Medicine, Karolinska Institutet, 2022-

Degrees and Education

Degree Of Doctor Of Philosophy, Department of Dental Medicine, Karolinska Institutet, 2012

About me

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