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Medal of Running Race

Bentolhoda Fereydouni

Postdoctoral researcher

Department and organisational affiliation:

About me

I received my PhD from  University of Goettingen, Germany in 2014. I performed my research study in German Primate Center, Department of Stem cell Biology. My research focus on In situ and In vitro analysis of germ and stem cell marker-positive cells in the postnatal ovary of common marmoset monkey (Callithrix jacchus).  

In 2015 I received  Marie-curie fellowship  for study DNA methylation on single cell level in sarcomas and oocytes in company TATAA biocentre, Gothenburg and secondment in Cambridge University , Babraham Institute ,Cambridge,  UK  then in 2016  I continue next postdoc in epigenetic study ChIP-qPCR and ChIP- seq in myxoid liposarcoma at Sahlgrenska Cancer center, University of Gothenburg  till 2018. during these 3 years I expanded my knowledge in epigenetic studies. For the next step in academia I chose Karolinska Institute for my further destination . 

In 2018 I joined  Karolinska Institute for single cell sequencing  .

Research description

2018-       Single cell sequencing , establishing dissociation system for 10x Chromium.

2016-2018 Cancer Center, Department of Pathology, University of Gothenburg. epigenetic study in MLS (myxoid liposarcoma), I established Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR to investigate the mechanism behind transcriptional regulation of SERPINB2  by the FUS-DDIT3 fusion oncoprotein from transfected HT1080 fibrosarcoma cells. Established ChIP-seq for the FET oncogenes and Interactions with SWI/SNF protein complex in MLS. worked with Patient-derived xenograft (PDX)model, transplanted subcutaneously MLS tomur to immunodeficient mice and performed IP injection of cancer stem cells in immunodeficient mice.Involved in JAK-STAT signalling controls cancer stem cell the project in MLS tomur.

2015-2016  single cell Bisulfite sequencing method in sarcomas and immature and maturehuman oocyte on single cell level and sequencing with NextSeq500 .TATAA Biocenter company, Gothenburg, Sweden, Secondment in Epigenetics Department, Babraham Institute, Cambridge Univesrity, Cambridgein Dr.Kelsey lab and collaboration with bioinformatic department Dr.Krueger for bioinformatic analysis with Bismark software.

2010- 2014 PhD project, Stem Cell Biology Unit, German Primate Center,Leibniz Institute for Primate Research, Goettingen, Germany. Supervisor: Prof. Dr. Rüdiger Behr. I used Immunohistochemistry and qPCR methods with pluripotency markers for recognizing oogonia subpopulation in neonatal  marmoset monkey and established novel long- term culture system  from primary cell culture of the neonatal ovaries for developing long term oocyte-like cells  (OLCs), using molecular and cell culture, microscopy techniques.Comparative transcriptome (bulk RNA seq) analysis of the cultured cells with different  passage colonies neonatal ovary, embryonic stem cells, and fibroblasts

 

Education

Doctoral Degree

2010-2014      Dr.rer.nat in Biology at Georg-August-University Goettingen, Goettingen, Germany.

                

Academic honours, awards and prizes

2018   Wilhelm och Martina Lundgrens Vetenskapsfond, “Study of IGF-1R as

 an anti-cancer target in sarcoma.”: 50000 SEK

2017-2018    Johan Janssons Foundation, “The FET oncogenes in sarcomas and

 Interactions with SWI/SNF protein complex”: 70000 SEK

2017-2018    Assar Gabrielssons Foundation, “The FET oncogenes in sarcomas

 and Interactions with SWI/SNF protein complex”: 54500 SEK

2016-2017    Assar Gabrielssons Foundation, “The FET oncogenes in sarcomas

 and Interactions with SWI/SNF protein complex”: 51000 SEK

2016-2017   Johan Janssons Foundation, “The FET oncogenes in sarcomas and

 Interactions with SWI/SNF protein complex”: 51000 SEK

2015           Marie Curie Fellowship as part of the EU-funded project to understand epigenetic regulation in the pathogenesis of common diseases 

2010-2014   DAAD (German Academic Exchange Service) scholarship for PhD study.

2010             Singapore International Graduate Award (SINGA), NUS. Singapore.

 

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