I am heading High Throughput Genome Engineering, a National SciLifeLab Facility offering pooled lentiviral CRISPR/Cas9 screens to the Swedish research community.
Pooled CRISPR/Cas9 screening enables parallel interrogation of thousands of protein coding genes, lncRNAs or other genetic elements for involvement in biological processes of interest. HTGE provides access to verified lentiviral CRISPR guide libraries for whole genome and more targeted loss- and gain of function studies (CRISPR knock-out, CRISPR inhibition, CRISPR activation). We also offer generation of stable Cas9-expressing lines in users’ cells of interest. HTGE supports the Swedish and international research community with CRISPR screening projects from planning to data analysis.
All our libraries are now barcoded, which is our own innovation and a major improvement compared to the conventional screening methodology (see our 2017 paper CRISPR/Cas9 screening using unique molecular identifiers).