Bernhard Schmierer

About me
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Grants
Public outreach and news

About me

I am heading CRISPR Functional Genomics (CFG), formerly High Throughput
Genome Engineering, a National SciLifeLab Facility offering massively
parallel CRISPR-Cas applications to the Swedish and international research
communities.

Research

Pooled CRISPR-Cas screening approaches enables parallel interrogation of
thousands of protein coding genes, lncRNAs or other genetic elements for
involvement in biological processes of interest. CFG provides access to
verified lentiviral CRISPR guide libraries for whole genome and targeted
loss- and gain of function studies (CRISPR knock-out, CRISPR inhibition,
CRISPR activation, base-editing mutagenesis screens). CFG offers support from
experimental design to data analysis.

Articles

Article: NATURE COMMUNICATIONS. 2023;14(1):5024

An idiosyncratic zonated stroma encapsulates desmoplastic liver metastases and originates from injured liver.

Fernández Moro C; Geyer N; Harrizi S; Hamidi Y; Söderqvist S; Kuznyecov D; Tidholm Qvist E; Salmonson Schaad M; Hermann L; Lindberg A; Heuchel RL; Martín-Bernabé A; Dhanjal S; Navis AC; Villard C; Del Valle AC; Bozóky L; Sparrelid E; Dirix L; Strell C; Östman A; Schmierer B; Vermeulen PB; Engstrand J; Bozóky B; Gerling M
Journal article: CANCER RESEARCH. 2023;83(7):3778

Genome-Scale CRISPRa and CRISPRi screening for lncRNA drivers of prostate cancer progression

Weiss S; Lord A; Schmierer B; Rovsing AB; Thomsen EA; Mikkelsen JG; Ulhoi B; Pedersen JS; Borre M; Sorensen KD
Journal article: CANCER RESEARCH. 2022;82(22_Supplement):a007

Abstract A007: Pancreatic cancer comprises co-existing transcriptional states regulated by distinct master regulator programs

Laise P; Turunen M; Maurer HC; Garcia AC; Elyada E; Schmierer B; Tomassoni L; Worley J; Alvarez MJ; Kesner J; Tan X; Tagore S; Fernandez EC; Wong K; Wang ALE; Ge S; Iuga AC; Griffin AT; Wong W; Manji GA; Notta F; Tuveson DA; Olive KPP; Califano A
Article: FRONTIERS IN ONCOLOGY. 2022;12:852980

Comprehensive Target Screening and Cellular Profiling of the Cancer-Active Compound b-AP15 Indicate Abrogation of Protein Homeostasis and Organelle Dysfunction as the Primary Mechanism of Action.

Gubat J; Selvaraju K; Sjöstrand L; Kumar Singh D; Turkina MV; Schmierer B; Sabatier P; Zubarev RA; Linder S; D'Arcy P
Article: ELIFE. 2021;10:e75415

Rapid genome editing by CRISPR-Cas9-POLD3 fusion.

Reint G; Li Z; Labun K; Keskitalo S; Soppa I; Mamia K; Tolo E; Szymanska M; Meza-Zepeda LA; Lorenz S; Cieslar-Pobuda A; Hu X; Bordin DL; Staerk J; Valen E; Schmierer B; Varjosalo M; Taipale J; Haapaniemi E
Article: JOURNAL OF CELL SCIENCE. 2019;132(14):jcs234039

TGF-β family ligands exhibit distinct signalling dynamics that are driven by receptor localisation.

Miller DSJ; Schmierer B; Hill CS
Article: NATURE COMMUNICATIONS. 2018;9(1):3664

Contribution of allelic imbalance to colorectal cancer.

Palin K; Pitkänen E; Turunen M; Sahu B; Pihlajamaa P; Kivioja T; Kaasinen E; Välimäki N; Hänninen UA; Cajuso T; Aavikko M; Tuupanen S; Kilpivaara O; van den Berg L; Kondelin J; Tanskanen T; Katainen R; Grau M; Rauanheimo H; Plaketti R-M; Taira A; Sulo P; Hartonen T; Dave K; Schmierer B; Botla S; Sokolova M; Vähärautio A; Gladysz K; Ongen H; Dermitzakis E; Bramsen JB; Ørntoft TF; Andersen CL; Ristimäki A; Lepistö A; Renkonen-Sinisalo L; Mecklin J-P; Taipale J; Aaltonen LA
Article: NATURE MEDICINE. 2018;24(7):927-930

CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response.

Haapaniemi E; Botla S; Persson J; Schmierer B; Taipale J
Article: MOLECULAR SYSTEMS BIOLOGY. 2017;13(10):945

CRISPR/Cas9 screening using unique molecular identifiers.

Schmierer B; Botla SK; Zhang J; Turunen M; Kivioja T; Taipale J
Article: NATURE COMMUNICATIONS. 2015;6:10050

Structural insights into the DNA-binding specificity of E2F family transcription factors.

Morgunova E; Yin Y; Jolma A; Dave K; Schmierer B; Popov A; Eremina N; Nilsson L; Taipale J
Article: SCIENCE SIGNALING. 2013;6(305):ra106

Controlling long-term signaling: receptor dynamics determine attenuation and refractory behavior of the TGF-β pathway.

Vizán P; Miller DSJ; Gori I; Das D; Schmierer B; Hill CS
Article: CELL. 2013;154(4):801-813

Transcription factor binding in human cells occurs in dense clusters formed around cohesin anchor sites.

Yan J; Enge M; Whitington T; Dave K; Liu J; Sur I; Schmierer B; Jolma A; Kivioja T; Taipale M; Taipale J
Article: BIOPHYSICAL JOURNAL. 2013;104(12):2595-2606

Dynamical scenarios for chromosome bi-orientation.

Zhang T; Oliveira RA; Schmierer B; Novák B
Article: OPEN BIOLOGY. 2011;1(3):110009

Cell cycle commitment in budding yeast emerges from the cooperation of multiple bistable switches.

Zhang T; Schmierer B; Novák B
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2011;286(9):7648-7660

Asparagine and aspartate hydroxylation of the cytoskeletal ankyrin family is catalyzed by factor-inhibiting hypoxia-inducible factor.

Yang M; Ge W; Chowdhury R; Claridge TDW; Kramer HB; Schmierer B; McDonough MA; Gong L; Kessler BM; Ratcliffe PJ; Coleman ML; Schofield CJ
Article: INTEGRATIVE BIOLOGY. 2011;3(3):197-207

Studying Smad2 intranuclear diffusion dynamics by mathematical modelling of FRAP experiments.

González-Pérez V; Schmierer B; Hill CS; Sear RP
Article: BMC SYSTEMS BIOLOGY. 2010;4:139

Hypoxia-dependent sequestration of an oxygen sensor by a widespread structural motif can shape the hypoxic response--a predictive kinetic model.

Schmierer B; Novák B; Schofield CJ
Article: DEVELOPMENT. 2008;135(17):2927-2937

Two highly related regulatory subunits of PP2A exert opposite effects on TGF-beta/Activin/Nodal signalling.

Batut J; Schmierer B; Cao J; Raftery LA; Hill CS; Howell M
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2008;105(18):6608-6613

Mathematical modeling identifies Smad nucleocytoplasmic shuttling as a dynamic signal-interpreting system.

Schmierer B; Tournier AL; Bates PA; Hill CS
Article: JOURNAL OF CELL SCIENCE. 2006;119(Pt 12):2435-2443

Upregulation of chicken p15INK4b at senescence and in the developing brain.

Kim S-H; Rowe J; Fujii H; Jones R; Schmierer B; Kong B-W; Kuchler K; Foster D; Ish-Horowicz D; Peters G
Article: MOLECULAR AND CELLULAR BIOLOGY. 2005;25(22):9845-9858

Kinetic analysis of Smad nucleocytoplasmic shuttling reveals a mechanism for transforming growth factor beta-dependent nuclear accumulation of Smads.

Schmierer B; Hill CS
Article: JOURNAL OF CELL SCIENCE. 2004;117(Pt 18):4113-4125

Analysis of Smad nucleocytoplasmic shuttling in living cells.

Nicolás FJ; De Bosscher K; Schmierer B; Hill CS
Article: BIOLOGY OF REPRODUCTION. 2004;70(5):1493-1499

Activin A and follicle-stimulating hormone control tight junctions in avian granulosa cells by regulating occludin expression.

Schuster MK; Schmierer B; Shkumatava A; Kuchler K
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2003;278(23):21197-21203

Activin a signaling induces Smad2, but not Smad3, requiring protein kinase a activity in granulosa cells from the avian ovary.

Schmierer B; Schuster MK; Shkumatava A; Kuchler K
Article: BIOLOGY OF REPRODUCTION. 2003;68(2):620-627

Activin and follicle-stimulating hormone signaling are required for long-term culture of functionally differentiated primary granulosa cells from the chicken ovary.

Schmierer B; Schuster MK; Shkumatava A; Kuchler K
Article: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 2000;274(2):542-547

Endothelial cells downregulate expression of the 70 kDa heat shock protein during hypoxia.

Oehler R; Schmierer B; Zellner M; Prohaska R; Roth E
Article: FASEB JOURNAL. 2000;14(9):1093-1100

Catecholamines up-regulate lipopolysaccharide-induced IL-6 production in human microvascular endothelial cells.

Gornikiewicz A; Sautner T; Brostjan C; Schmierer B; Függer R; Roth E; Mühlbacher F; Bergmann M
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All other publications

Book chapter: GENOME EDITING IN DRUG DISCOVERY. 2022;p. 151-164

Sequence Diversification Screens with CRISPR‐Cas9‐Guided Base Editors

Persson J; Schmierer B
Conference publication: CANCER RESEARCH. 2022;82(22):5

Pancreatic cancer comprises co-existing transcriptional states regulated by distinct master regulator programs.

Laise P; Turunen M; Maurer HC; Garcia AC; Elyada E; Schmierer B; Tomassoni L; Worley J; Alvarez MJ; Kesner J; Tan X; Tagore S; Fernandez EC; Wong K; Wang ALE; Ge S; Iuga AC; Griffin AT; Wong W; Manji GA; Notta F; Tuveson DA; Olive KPP; Califano A
Conference publication: MOLECULAR THERAPY. 2022;30(4):267

Rapid Genome Editing by Cas9-POLD3 Fusion

Reint G; Li Z; Labun K; Keskitalo S; Soppa I; Mamia K; Tolo E; Szymanska M; Meza-Zepeda L; Lorenz S; Cieslar-Pobuda A; Hu X; Bordin D; Staerk J; Valen E; Schmierer B; Varjosalo M; Taipale J; Haapaniemi E
Preprint: BIORXIV. 2021

Rapid genome editing by CRISPR-Cas9-POLD3 fusion

Reint G; Li Z; Labun K; Keskitalo S; Soppa I; Mamia K; Tölö E; Szymanska M; Meza-Zepeda LA; Lorenz S; Cieslar-Pobuda A; Hu X; Bordin DL; Staerk J; Valen E; Schmierer B; Varjosalo M; Taipale J; Haapaniemi E
Preprint: BIORXIV. 2020

Developmental and MAPK-responsive transcription factors drive distinct malignant subtypes and genetic dependencies in pancreatic cancer

Laise P; Turunen M; Garcia AC; Tomassoni L; Maurer C; Elyada E; Schmierer B; Worley J; Kesner J; Tan X; Fernandez EC; Wong K; Wasko U; Tagore S; Wang A; Ge S; Iuga A; Griffin A; Wong W; Manji G; Alvarez M; Notta F; Tuveson D; Olive K; Califano A
Review: COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL. 2020;18:2237-2246

Designing custom CRISPR libraries for hypothesis-driven drug target discovery.

Iyer VS; Jiang L; Shen Y; Boddul SV; Panda SK; Kasza Z; Schmierer B; Wermeling F
Letter: MOLECULAR SYSTEMS BIOLOGY. 2019;15(8):e9059

Reply to "CRISPR screens are feasible in TP53 wild-type cells".

Haapaniemi E; Botla S; Persson J; Schmierer B; Taipale J
Preprint: BIORXIV. 2019

TGF-β family ligands exhibit distinct signaling dynamics that are driven by receptor localization

Miller D; Schmierer B; Hill C
Preprint: BIORXIV. 2017

Inhibition of p53 improves CRISPR/Cas - mediated precision genome editing

Haapaniemi E; Botla S; Persson J; Schmierer B; Taipale J
Preprint: BIORXIV. 2017

CRISPR/Cas9 screening using unique molecular identifiers

Schmierer B; Botla SK; Zhang J; Turunen M; Kivioja T; Taipale J
Letter: SCIENCE SIGNALING. 2014;7(344):lc2

Response to comment on "Controlling long-term signaling: receptor dynamics determine attenuation and refractory behavior of the TGF-β pathway"-Smad2/3 activity does not predict the dynamics of transcription.

Vizán P; J Miller DS; Schmierer B; Hill CS
Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 29

Anoxia

Schmierer B
Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 311-314

Cell Cycle Signaling, Hypoxia

Schmierer B
Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 1055-1056

Ischemic Disease

Schmierer B
Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 1846

Reoxygenation

Schmierer B
Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 1802

PVHL

Schmierer B
Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 434

c-Myc

Schmierer B
Corrigendum: INTEGRATIVE BIOLOGY. 2011;3(12):1233

Studying Smad2 intranuclear diffusion dynamics by mathematical modelling of FRAP experiments (vol 3, pg 197, 2011)

Gonzalez-Perez V; Schmierer B; Hill CS; Sear RP
Review: NATURE REVIEWS MOLECULAR CELL BIOLOGY. 2007;8(12):970-982

TGFbeta-SMAD signal transduction: molecular specificity and functional flexibility.

Schmierer B; Hill CS
Book chapter: SMAD SIGNAL TRANSDUCTION. 2006;p. 193-213

Nucleocytoplasmic Shuttling of Smad Proteins

Schmierer B; Hill CS

Grants

Gene Knock Up at the post-transcriptional level to model and treat neurological disease

Swedish Research Council

1 January 2023 - 31 December 2025
Differentiation state instability and clonal selection as escape paths from anticancer therapy

Swedish Research Council

1 January 2021 - 31 December 2023
Mechanism of Smad2/3 nucleocytoplasmic shuttling

FWF Austrian Science Fund

1 June 2004 - 31 May 2006

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