Erdinc Sezgin

Erdinc Sezgin

Assistant professor

About me

I am a biophysicist eager to understand biology by using the power of physics and chemistry. We cheer for curiosity-driven research, open science, public science communication and equality and diversity in science.  

Research description

Our Lab works on physicochemical principles underlying cellular processes.

Cells in our body communicate with each other with complex signaling events. Although these signaling events involve a multitude of signal transduction molecules, they are usually triggered by interactions between a ligand and a receptor in the plasma membrane. Investigation of spatiotemporal interactions between the signaling components in the membrane has so far been hampered by the complex structure of the cell membrane. The processes driving signaling in the plasma membrane involve not only the core protein components (such as receptors and ligands), but also membrane lipids, the underlying actin cytoskeleton, glycocalyx covering the membrane, intracellular protein scaffolds, and downstream interactors. This complexity is a major technical challenge in attempting to discern the impact of each individual component on the various steps of immune cell signaling. Related to this, in this vast complexity, it is very challenging to deduce insights on the physicochemical principles behind these processes.  Moreover, early phases of signaling where the ligands meet the receptors occur at very fast temporal (milliseconds) and small spatial (nanometers) scales, which further hampers thorough elucidation of these processes. Therefore, studies aiming at a mechanistic understanding of immune signaling processes require both advanced imaging techniques with nanometer and microsecond spatiotemporal resolution and well-defined biomimetic systems that can provide tuneable molecular specificity and complexity to pinpoint the role of each functional component as well as physical principles of signaling processes.

In our lab, we solve these problems by developing and using advanced imaging and synthetic biology tools to reveal the molecular mechanisms governing the cellular signaling.

 

Key publications:

Please see Erdinc's Google Scholar for full publication list.

Beckers D, Urbancic D, Sezgin E, Impact of nanoscale hindrances on the relationship between lipid packing and diffusion in model membranes, Journal of Physical Chemistry B (2020)​​​​​

Pinkwart K, Schneider F, Lukoseviciute M, Sauka-Spengler T, Lyman E, Eggeling C, Sezgin E. Nanoscale dynamics of cholesterol in the cell membrane. Journal of Biological Chemistry, (2019)​​​​​

Sezgin E, Schneider F, Galiani S, Urbančič I, Waithe D, Lagerholm BC, Eggeling C. Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED-FCS. Nature Protocols, 14(4), 1054-1083, (2019)

Jenkins E, Santos AM, O'Brien-Ball C, Felce JH, Wilcock MJ, Hatherley D, Dustin ML, Davis SJ, Eggeling C, Sezgin E. Reconstitution of immune cell interactions in free-standing membranes. Journal of Cell Science, 132(4), (2018)

Felce JH*, Sezgin E*, Wane M, Brouwer H, Dustin ML, Eggeling C, Davis SJ. CD45 exclusion- and cross-linking-based receptor signaling together broaden FcεRI reactivity. Science Signalling, 11(561), (2018)

Sezgin E, Levental I, Mayor S, Eggeling C. The mystery of membrane organization: composition, regulation and roles of lipid rafts Nature Reviews Molecular Cell Biology, (2017)

Schneider F, Waithe D, Clausen MP, Galiani S, Koller T, Ozhan G, Eggeling C, Sezgin E. Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS. Molecular Biology of the Cell, (2017) 

Sezgin E, F. Schneider, V. Zilles, I. Urbancic, E. Garcia D. Waithe, A. S. Klymchenko, C. Eggeling Polarity sensitive probes for super resolution STED microscopy.  Biophysical Journal, (2017) 

F. Schenider, D. Waithe, S. Galiani, J.B. de la Serna, E. Sezgin*, C. Eggeling* Nanoscale spatio-temporal diffusion modes measured by simultaneous confocal and STED imaging. Nano Letters, 18(7), 4233–4240, (2018)

 

Teaching portfolio

  • 2015-2019: University of Oxford, Cellular Imaging Course for MSc Immunology students
  • 2015-2019: University of Oxford, Methods and Techniques Course for PhD students
  • 2016-2019: Heidelberg, Germany, EMBO Super-resolution Microscopy Workshop
  • 2017: University of Oxford, Research Techniques Course for Medical Sciences PhD students
  • 2017: University of Ljubljana, Advanced Microscopy Workshop
  • 2016-2017: Izmir, Turkey, Cellular Imaging Techniques Workshop
  • 2016: Montpellier, France, Advanced Fluorescence Fluctuation Methods in FBIAT Advanced BioImaging Workshop
  • 2010-2012: Technical University Dresden, Teaching Assistant for Biophysical Methods for Nanobiophysics and Molecular Bioengineering Master Students

Education

2014-2017 Postdoctoral Scientist University of Oxford Supervisor: Prof. Christian Eggeling

2013 May-2014 Postdoctoral Scientist Max Planck Institute of Cell Biology and Genetics Supervisor: Prof. Kai Simons

 

2010-2013 PhD International Max Planck Research School (IMPRS) Dresden International PhD Program (DIPP) Supervisor: Prof. Petra Schwille

2004-2009 BSc Yeditepe University Department of Genetics and Bioengineering Supervisor: Prof. Mustafa Culha

Academic honours, awards and prizes

  • SciLifeLab Fellowship, 2020-2025
  • Wellcome Trust ISSF Grant, 2019-2020
  • British Council Newton Fellowship (co-applicant, lead researcher), 2018-2020
  • Marie Curie Intra-European Postdoctoral Fellowship, 2015-2017
  • EMBO Long Term Postdoctoral Fellowship, 2014-2015
  • Equality and Diversity in Science Award (WIMM Oxford), 2019
  • Excellence Award (RDM Oxford), 2016 and 2018 (Twice)
  • Oxford Public Engagement Award, 2016
  • Oxford Image Competition Award, 2016 and 2017 (Twice)
  • British Council Workshop Grants Award, 2016 and 2017 (Twice)
  • ICGEB Workshop Grant Award, 2015
  • Dresden International PhD Program (DIPP) Travel Award, 2013
  • First Honor Graduate from Yeditepe University with GPA 3.92/4.00 , 2009
  • Yeditepe University Scholarship for Top 0.5% in University Entrance Examination, 2004-2009
  • Turkish Government Scholarship for University Education, 2004-2009
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