Fanglei Zuo

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About me

o 2023-now Research Specialist, Division of Immunology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Sweden. Projects: Vaccine and antibody development
o 2020-2023 Postdoctoral researcher, Department of Biosciences and Nutrition, Karolinska Institutet, Sweden. Projects: antibody therapy against COVID-19 (the ATAC project) and mucosal immunity against SARS-CoV-2
engineering lactobacilli for delivering antibodies against Helicobacter pylori and norovirus
o 2017-2020 Postdoctoral researcher, Department of Molecular Biosciences, The Wenner-Gren Institute (MBW), Stockholm University, Sweden. Project: understanding the molecular mechanisms by which lactobacilli antagonize Helicobacter pylori
o 2015-2017 Postdoctoral researcher, Department of Laboratory Medicine, Karolinska Institutet, Sweden. Project: engineering lactobacilli for delivering therapeutic molecules against mucosal pathogens including HIV and norovirus
o 2008-2014 Pre-doctoral fellow, College of Food Science and Nutritional Engineering, China Agricultural University, China. Project: stress physiology of probiotic lactobacilli and bifidobacteria

Research

I am currently mainly working on immunity and antibody therapy against COVID-19. The main task is to evaluate the dynamics of the systemic and mucosal SARS-CoV-2-specific antibodies from infected and vaccinated individuals, and to develop antibody-based passive immunotherapy and vaccines against pancoronavirus.

Articles

Article: NATURE COMMUNICATIONS. 2023;14(1):6527

Design, structure and plasma binding of ancestral β-CoV scaffold antigens.

Hueting D; Schriever K; Sun R; Vlachiotis S; Zuo F; Du L; Persson H; Hofström C; Ohlin M; Walldén K; Buggert M; Hammarström L; Marcotte H; Pan-Hammarström Q; Andréll J; Syrén P-O
Article: ISCIENCE. 2023;26(4):106562

19n01, a broadly neutralizing antibody against omicron BA.1, BA.2, BA.4/5, and other SARS-CoV-2 variants of concern.

García-Vega M; Melgoza-González EA; Hernández-Valenzuela S; Hinojosa-Trujillo D; Reséndiz-Sandoval M; Llamas-Covarrubias MA; Loza-López M; Valenzuela O; Soto-Gaxiola A; Hernández-Oñate MA; Mata-Haro V; Cassaniti I; Sammartino JC; Ferrari A; Simonelli L; Pedotti M; Sun R; Zuo F; Baldanti F; Varani L; Marcotte H; Pan-Hammarström Q; Hernández J
Article: PHARMACEUTICS. 2022;15(1):63

Lactobacilli as a Vector for Delivery of Nanobodies against Norovirus Infection.

Yuki Y; Zuo F; Kurokawa S; Uchida Y; Sato S; Sakon N; Hammarström L; Kiyono H; Marcotte H
Article: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2022;23(24):15451

Lactobacilli Downregulate Transcription Factors in Helicobacter pylori That Affect Motility, Acid Tolerance and Antimicrobial Peptide Survival.

Zuo F; Somiah T; Gebremariam HG; Jonsson A-B
Article: SCIENTIFIC REPORTS. 2022;12(1):20064

Lactate causes downregulation of Helicobacter pylori adhesin genes sabA and labA while dampening the production of proinflammatory cytokines.

Somiah T; Gebremariam HG; Zuo F; Smirnova K; Jonsson A-B
Article: JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY. 2022;150(5):1059-1073

Genetic and immunologic evaluation of children with inborn errors of immunity and severe or critical COVID-19.

Abolhassani H; Delavari S; Landegren N; Shokri S; Bastard P; Du L; Zuo F; Hajebi R; Abolnezhadian F; Iranparast S; Modaresi M; Vosughimotlagh A; Salami F; Aranda-Guillén M; Cobat A; Marcotte H; Zhang S-Y; Zhang Q; Rezaei N; Casanova J-L; Kämpe O; Hammarström L; Pan-Hammarström Q
Article: NATURE COMMUNICATIONS. 2022;13(1):2670

Heterologous immunization with inactivated vaccine followed by mRNA-booster elicits strong immunity against SARS-CoV-2 Omicron variant.

Zuo F; Abolhassani H; Du L; Piralla A; Bertoglio F; de Campos-Mata L; Wan H; Schubert M; Cassaniti I; Wang Y; Sammartino JC; Sun R; Vlachiotis S; Bergami F; Kumagai-Braesch M; Andréll J; Zhang Z; Xue Y; Wenzel EV; Calzolai L; Varani L; Rezaei N; Chavoshzadeh Z; Baldanti F; Hust M; Hammarström L; Marcotte H; Pan-Hammarström Q
Article: J ALLERGY CLIN IMMUNOL GLOB. 2022;1(2):37-42

Lung function before and after COVID-19 in young adults: A population-based study.

Mogensen I; Hallberg J; Björkander S; Du L; Zuo F; Hammarström L; Pan-Hammarström Q; Ekström S; Georgelis A; Palmberg L; Janson C; Bergström A; Melén E; Kull I; BAMSE COVID-19 Study Group
Article: JOURNAL OF CLINICAL IMMUNOLOGY. 2022;42(3):471-483

Inherited IFNAR1 Deficiency in a Child with Both Critical COVID-19 Pneumonia and Multisystem Inflammatory Syndrome.

Abolhassani H; Landegren N; Bastard P; Materna M; Modaresi M; Du L; Aranda-Guillén M; Sardh F; Zuo F; Zhang P; Marcotte H; Marr N; Khan T; Ata M; Al-Ali F; Pescarmona R; Belot A; Béziat V; Zhang Q; Casanova J-L; Kämpe O; Zhang S-Y; Hammarström L; Pan-Hammarström Q
Article: BMC MEDICINE. 2022;20(1):102

Human serum from SARS-CoV-2-vaccinated and COVID-19 patients shows reduced binding to the RBD of SARS-CoV-2 Omicron variant.

Schubert M; Bertoglio F; Steinke S; Heine PA; Ynga-Durand MA; Maass H; Sammartino JC; Cassaniti I; Zuo F; Du L; Korn J; Milošević M; Wenzel EV; Krstanović F; Polten S; Pribanić-Matešić M; Brizić I; Baldanti F; Hammarström L; Dübel S; Šustić A; Marcotte H; Strengert M; Protić A; Piralla A; Pan-Hammarström Q; Čičin-Šain L; Hust M
Article: ISCIENCE. 2022;25(2):103743

Immunity to SARS-CoV-2 up to 15 months after infection.

Marcotte H; Piralla A; Zuo F; Du L; Cassaniti I; Wan H; Kumagai-Braesh M; Andréll J; Percivalle E; Sammartino JC; Wang Y; Vlachiotis S; Attevall J; Bergami F; Ferrari A; Colaneri M; Vecchia M; Sambo M; Zuccaro V; Asperges E; Bruno R; Oggionni T; Meloni F; Abolhassani H; Bertoglio F; Schubert M; Calzolai L; Varani L; Hust M; Xue Y; Hammarström L; Baldanti F; Pan-Hammarström Q
Article: JOURNAL OF CLINICAL IMMUNOLOGY. 2022;42(1):1-9

X-Linked TLR7 Deficiency Underlies Critical COVID-19 Pneumonia in a Male Patient with Ataxia-Telangiectasia.

Abolhassani H; Vosughimotlagh A; Asano T; Landegren N; Boisson B; Delavari S; Bastard P; Aranda-Guillén M; Wang Y; Zuo F; Sardh F; Marcotte H; Du L; Zhang S-Y; Zhang Q; Rezaei N; Kämpe O; Casanova J-L; Hammarström L; Pan-Hammarström Q
Article: JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY. 2022;149(1):65-75.e8

SARS-CoV-2-specific B- and T-cell immunity in a population-based study of young Swedish adults.

Björkander S; Du L; Zuo F; Ekström S; Wang Y; Wan H; Sherina N; Schoutens L; Andréll J; Andersson N; Georgelis A; Bergström A; Marcotte H; Kull I; Hammarström L; Melén E; Pan-Hammarström Q; BAMSE COVID-19 study group
Article: INFECTION AND IMMUNITY. 2021;89(10):e0029621-e00221

Lactate-Induced Dispersal of Neisseria meningitidis Microcolonies Is Mediated by Changes in Cell Density and Pilus Retraction and Is Influenced by Temperature Change.

Sigurlásdóttir S; Lidberg K; Zuo F; Newcombe J; McFadden J; Jonsson A-B
Article: APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2019;85(19):e00800-e00819

Putative Adhesion Factors in Vaginal Lactobacillus gasseri DSM 14869: Functional Characterization.

Zeng Z; Zuo F; Marcotte H
Article: BMC INFECTIOUS DISEASES. 2019;19(1):824

An exploratory pilot study evaluating the supplementation of standard antibiotic therapy with probiotic lactobacilli in south African women with bacterial vaginosis.

Marcotte H; Larsson PG; Andersen KK; Zuo F; Mikkelsen LS; Brandsborg E; Gray G; Laher F; Otwombe K
Article: ACS SYNTHETIC BIOLOGY. 2019;8(8):1723-1729

Inducible Plasmid Self-Destruction (IPSD) Assisted Genome Engineering in Lactobacilli and Bifidobacteria.

Zuo F; Zeng Z; Hammarström L; Marcotte H
Article: NUTRIENTS. 2019;11(4):E782-782

Exopolysaccharide-Producing Bifidobacterium adolescentis Strains with Similar Adhesion Property Induce Differential Regulation of Inflammatory Immune Response in Treg/Th17 Axis of DSS-Colitis Mice.

Yu R; Zuo F; Ma H; Chen S
Article: FRONTIERS IN MICROBIOLOGY. 2019;10:2770

Role of Sortase A in Lactobacillus gasseri Kx110A1 Adhesion to Gastric Epithelial Cells and Competitive Exclusion of Helicobacter pylori.

Zuo F; Appaswamy A; Gebremariam HG; Jonsson A-B
Article: FRONTIERS IN MICROBIOLOGY. 2019;10:422

Deletion of D-Lactate Dehydrogenase A in Neisseria meningitidis Promotes Biofilm Formation Through Increased Autolysis and Extracellular DNA Release.

Sigurlásdóttir S; Wassing GM; Zuo F; Arts M; Jonsson A-B
Article: SCIENTIFIC REPORTS. 2018;8(1):17085

Transcriptomic analysis of Bifidobacterium longum subsp. longum BBMN68 in response to oxidative shock.

Zuo F; Yu R; Xiao M; Khaskheli GB; Sun X; Ma H; Ren F; Zhang B; Chen S
Article: MICROBIOLOGICAL RESEARCH. 2017;205:88-98

Characterization and complete genome sequences of L. rhamnosus DSM 14870 and L. gasseri DSM 14869 contained in the EcoVag® probiotic vaginal capsules.

Marcotte H; Krogh Andersen K; Lin Y; Zuo F; Zeng Z; Larsson PG; Brandsborg E; Brønstad G; Hammarström L
Article: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2017;101(19):7177-7186

Recombinant Lactococcus lactis expressing bioactive exendin-4 to promote insulin secretion and beta-cell proliferation in vitro.

Zeng Z; Yu R; Zuo F; Zhang B; Ma H; Chen S
Article: FEMS MICROBIOLOGY LETTERS. 2017;364(16):fnx167

Characterization of a lactose-responsive promoter of ATP-binding cassette (ABC) transporter gene from Lactobacillus acidophilus 05-172.

Zeng Z; Zuo F; Yu R; Zhang B; Ma H; Chen S
Journal article: ANNALS OF MICROBIOLOGY. 2016;66(3):1027-1037

Characterization and in vitro properties of potential probiotic Bifidobacterium strains isolated from breast-fed infant feces

Zuo F; Yu R; Feng X; Chen L; Zeng Z; Khaskheli GB; Ma H; Chen S
Article: MICROBIAL CELL FACTORIES. 2016;15(1):102

Recombinant Lactococcus lactis NZ9000 secretes a bioactive kisspeptin that inhibits proliferation and migration of human colon carcinoma HT-29 cells.

Zhang B; Li A; Zuo F; Yu R; Zeng Z; Ma H; Chen S
Article: LETTERS IN APPLIED MICROBIOLOGY. 2016;62(3):250-255

Bifidobacteria possess inhibitory activity against dipeptidyl peptidase-IV.

Zeng Z; Luo JY; Zuo FL; Yu R; Zhang Y; Ma HQ; Chen SW
Article: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY. 2016;178(3):451-461

The Native Plasmid pML21 Plays a Role in Stress Tolerance in Enterococcus faecalis ML21, as Analyzed by Plasmid Curing Using Plasmid Incompatibility.

Zuo F-L; Chen L-L; Zeng Z; Feng X-J; Yu R; Lu X-M; Ma H-Q; Chen S-W
Article: INTERNATIONAL DAIRY JOURNAL. 2016;53:37-44

Comparison of dipeptidyl peptidase IV-inhibitory activity of peptides from bovine and caprine milk casein by in silico and in vitro analyses

Zhang Y; Chen R; Zuo F; Ma H; Zhang Y; Chen S
Article: PLOS ONE. 2016;11(10):e0165130

Heterologous Expression and Delivery of Biologically Active Exendin-4 by Lactobacillus paracasei L14.

Zeng Z; Yu R; Zuo F; Zhang B; Peng D; Ma H; Chen S
Journal article: JOURNAL OF FUNCTIONAL FOODS. 2016;20:486-495

Screening for potential novel probiotic Lactobacillus strains based on high dipeptidyl peptidase IV and α-glucosidase inhibitory activity

Zeng Z; Luo J; Zuo F; Zhang Y; Ma H; Chen S
Article: SCIENTIFIC REPORTS. 2015;5:14109

Comparative genome-based identification of a cell wall-anchored protein from Lactobacillus plantarum increases adhesion of Lactococcus lactis to human epithelial cells.

Zhang B; Zuo F; Yu R; Zeng Z; Ma H; Chen S
Article: CURRENT MICROBIOLOGY. 2015;71(1):8-15

Overexpression of Small Heat Shock Protein Enhances Heat- and Salt-Stress Tolerance of Bifidobacterium longum NCC2705.

Khaskheli GB; Zuo F; Yu R; Chen S
Article: LETTERS IN APPLIED MICROBIOLOGY. 2014;59(5):549-556

Identification and partial characterization of lactic acid bacteria isolated from traditional dairy products produced by herders in the western Tianshan Mountains of China.

Zuo FL; Feng XJ; Chen LL; Chen SW
Article: RESEARCH IN MICROBIOLOGY. 2014;165(7):581-589

Homologous overexpression of alkyl hydroperoxide reductase subunit C (ahpC) protects Bifidobacterium longum strain NCC2705 from oxidative stress.

Zuo F; Yu R; Khaskheli GB; Ma H; Chen L; Zeng Z; Mao A; Chen S
Article: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2014;98(17):7523-7534

Combination of heterogeneous catalase and superoxide dismutase protects Bifidobacterium longum strain NCC2705 from oxidative stress.

Zuo F; Yu R; Feng X; Khaskheli GB; Chen L; Ma H; Chen S
Article: JOURNAL OF FOOD BIOCHEMISTRY. 2012;36(6):706-717

EFFECT OF VARYING LEVELS OF ADDED CALCIUM ON THE BIOCHEMISTRY, TEXTURE AND MICRO-STRUCTURE OF FRESH CHEESE CONTAINING SESAME PROTEIN ISOLATE

Lu XM; Zuo FL; Zhao JY; Guo HY; Ren F; Chen SW
Article: MICROBIOLOGY-SGM. 2011;157(Pt 6):1573-1588

Oxidative stress-related responses of Bifidobacterium longum subsp. longum BBMN68 at the proteomic level after exposure to oxygen.

Xiao M; Xu P; Zhao J; Wang Z; Zuo F; Zhang J; Ren F; Li P; Chen S; Ma H
Article: JOURNAL OF BACTERIOLOGY. 2011;193(3):787-788

Complete genome sequence of Bifidobacterium longum subsp. longum BBMN68, a new strain from a healthy chinese centenarian.

Hao Y; Huang D; Guo H; Xiao M; An H; Zhao L; Zuo F; Zhang B; Hu S; Song S; Chen S; Ren F
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Editorial comment: LANCET INFECTIOUS DISEASES. 2024;24(1):e7-e9

Neutralisation activity of mucosal IgA against XBB sublineages and BA.2.86.

Zuo F; Cao Y; Sun R; Yisimayi A; Du L; Bertoglio F; Schubert M; Guerra C; Cavalli A; Hust M; Robbiani DF; Abolhassani H; Xie XS; Hammarström L; Marcotte H; Pan-Hammarström Q
Preprint: BIORXIV. 2023

Conversion of monoclonal IgG to dimeric and secretory IgA restores neutralizing ability and prevents infection of Omicron lineages

Marcotte H; Cao Y; Zuo F; Simonelli L; Sammartino JC; Pedotti M; Sun R; Cassaniti I; Hagbom M; Piralla A; Yang J; Du L; Percivalle E; Bertoglio F; Schubert M; Abolhassani H; Sherina N; Guerra C; Borte S; Razaei N; Kumagai-Braesch M; Xue Y; Grönwall C; Klareskog L; Calzolai L; Cavalli A; Wang Q; Robbiani D; Hust M; Shi Z; Feng L; Svensson L; Chen L; Bao L; Baldanti F; Qin C; Xiao J; Hammarström L; Yang XL; Varani L; Xie XS; Pan-Hammarström Q
Letter: LANCET REGIONAL HEALTH-WESTERN PACIFIC. 2023;33:100762

Heterologous inactivated virus/mRNA vaccination response to BF.7, BQ.1.1, and XBB.1.

Zuo F; Sun R; Abolhassani H; Du L; Wang Y; Vlachiotis S; Bertoglio F; Schubert M; Rezaei N; Chavoshzadeh Z; Guerra C; Cavalli A; Andréll J; Kumagai-Braesch M; Xue Y; Cao Y; Hust M; Robbiani DF; Xie XS; Hammarström L; Marcotte H; Pan-Hammarström Q
Preprint: MEDRXIV. 2023

Heterologous inactivated virus/mRNA vaccination response to BF.7, BQ.1.1, and XBB.1

Zuo F; Sun R; Abolhassani H; Du L; Wang Y; Vlachiotis S; Bertoglio F; Schubert M; Rezaei N; Chavoshzadeh Z; Guerra C; Cavalli A; Andréll J; Kumagai-Braesch M; Xue Y; Cao Y; Hust M; Robbiani D; Xie XS; Hammarström L; Marcotte H; Pan-Hammarström Q
Letter: NEW ENGLAND JOURNAL OF MEDICINE. 2022;387(21):e55

Mucosal IgA against SARS-CoV-2 Omicron Infection. Reply.

Åberg M; Smed-Sörensen A; Thålin C
Letter: NEW ENGLAND JOURNAL OF MEDICINE. 2022;387(21):e55

Mucosal IgA against SARS-CoV-2 Omicron Infection.

Zuo F; Marcotte H; Hammarström L; Pan-Hammarström Q
Preprint: RESEARCH SQUARE. 2022

Design, structure and plasma binding of ancestral β-CoV scaffold antigens

Syren P-O; Hueting D; Schriever K; Zuo F; Du L; Persson H; Hofström C; Ohlin M; Wallden K; Hammarstrom L; Marcotte H; Pan-Hammarstrom Q; Andrell J
Preprint: MEDRXIV. 2022

Heterologous immunization with inactivated vaccine followed by mRNA booster elicits strong humoral and cellular immune responses against the SARS-CoV-2 Omicron variant

Zuo F; Abolhassani H; Du L; Piralla A; Bertoglio F; de Campos-Mata L; Wan H; Schubert M; Wang Y; Sun R; Cassaniti I; Vlachiotis S; Kumagai-Braesch M; Andréll J; Zhang Z; Xue Y; Wenzel EV; Calzolai L; Varani L; Rezaei N; Chavoshzadeh Z; Baldanti F; Hust M; Hammarström L; Marcotte H; Pan-Hammarström Q
Editorial comment: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY. 2022;12:938282

Editorial: Probiotics - A New Tool for Restraining Infectious Pathogens and Antibiotic Resistance.

Zuo F; Liu G; Jin J
Preprint: SSRN. 2022

19n01, a Broadly Neutralizing Antibody Against Omicron BA.1, BA.2, BA.4/5, and Other SARS-CoV-2 Variants of Concern

García-Vega M; Melgoza-González E; Hernández-Valenzuela S; Hinojosa-Trujillo D; Reséndiz-Sandoval M; Llamas-Covarrubias MA; Loza-Lopez M; Valenzuela O; Soto-Gaxiola A; Hernández-Oñate M; Mata-Haro V; Cassaniti I; Sammartino JC; Ferrari A; Simonelli L; Pedotti M; Sun R; Zuo F; Baldanti F; Varani L; Marcotte H; Pan-Hammarström Q; Hernández J
Preprint: MEDRXIV. 2021

Human serum from SARS-CoV-2 vaccinated and COVID-19 patients shows reduced binding to the RBD of SARS-CoV-2 Omicron variant

Schubert M; Bertoglio F; Steinke S; Heine PA; Ynga-Durand MA; Zuo F; Du L; Korn J; Milošević M; Wenzel EV; Maass H; Krstanović F; Polten S; Pribanić-Matešić M; Brizić I; Piralla A; Baldanti F; Hammarström L; Dübel S; Šustić A; Marcotte H; Strengert M; Protić A; Pan-Hammarström Q; Čičin-Šain L; Hust M
Preprint: BIORXIV. 2021

Immunity to SARS-CoV-2 up to 15 months after infection

Marcotte H; Piralla A; Zuo F; Du L; Cassaniti I; Wan H; Kumagai-Braesh M; Andréll J; Percivalle E; Sammartino JC; Wang Y; Vlachiotis S; Attevall J; Bergami F; Ferrari A; Colaneri M; Vecchia M; Sambo M; Zuccaro V; Asperges E; Bruno R; Oggionni T; Meloni F; Abolhassanni H; Bertoglio F; Schubert M; Calzolai L; Varani L; Hust M; Xue Y; Hammarström L; Baldanti F; Pan-Hammarström Q
Review: CURRENT OPINION IN BIOTECHNOLOGY. 2021;70:75-82

Advancing mechanistic understanding and bioengineering of probiotic lactobacilli and bifidobacteria by genome editing.

Zuo F; Marcotte H
Review: BIOTECHNOLOGY ADVANCES. 2020;45:107654

Engineer probiotic bifidobacteria for food and biomedical applications - Current status and future prospective.

Zuo F; Chen S; Marcotte H
Preprint: BIORXIV. 2019

Inducible Plasmid Self-Destruction (IPSD) assisted genome engineering in lactobacilli and bifidobacteria

Zuo F; Zeng Z; Hammarström L; Marcotte H
Editorial comment: CURRENT MICROBIOLOGY. 2013;66(2):103-105

Characterization of plasmid pML21 of Enterococcus faecalis ML21 from koumiss.

Zuo F; Feng X; Sun X; Du C; Chen S

Grants

Stiftelsen Clas Groschinskys Minnesfond
Eva & Oscar Ahréns Stiftelse
Petrus och Augusta Hedlunds stiftelse
Magn Bergvalls Stiftelse
Tore Nilsons Stiftelse För Medicinsk Forskning
Ruth and Richard Julin Foundation
KI Research Foundations Grants
Stiftelsen Läkare mot AIDS Forskningsfond
Sven och Lilly Lawskis fond

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