Felipe De Oliveira Galvão
Research specialist
About me
Marcos Felipe de Oliveira Galvão received his M.Sc. degree in Biochemistry in 2011 and a Ph.D. degree in Biochemistry and Molecular Biology in 2016 from Professor Silvia Batistuzzo’s group, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil. In 2015 he was awarded for a Ph.D. visiting fellowship at the unit of Biochemical Toxicology, Institute of Environmental Medicine (IMM), Karolinska Institutet, Sweden. In 2018 he joined Professor Kristian Dreij’s group for a postdoc focusing on Genetic Toxicology and in 2023 he got a position as Researcher Specialist at IMM.
He has experience in Genetic Toxicology and Environmental Mutagenesis with emphasis on particulate matter sampling, physical-chemical characterization, air pollutants dispersion modeling, and analysis of in vitro and in vivo effects of air pollutants. The focus of his research is to assess the impact of complex mixtures emitted by biomass burning in both an occupational and environmental exposure scenario.
Research description
Development of new approach methodologies for assessing cancer risks associated with air pollution mixtures.
Air pollution is a complex mixture of compounds with different biological activities that makes risk assessment a challenge. Air pollution in general, as well as many of its components, is classified as carcinogens. Current strategies for cancer risk assessment (CRA) of air pollution are however based on a pollutant-by-pollutant approach. This is a great simplification and excludes the possibility of mixture effects that may underestimate actual human health risks. The objective of this project is to address these issues by developing new approach methodologies (NAMs) for CRA of air pollution mixtures. We suggest that CRA of complex mixtures should be based on toxicity testing of whole mixtures in vitro and determination of relative Mixture Potency Factors (MPFs) (Jarvis et al 2013, Jarvis et al 2014). We have also showed that this approach can accurately determine genotoxic potency of single air pollutants (Lim et al 2015, Dreij et al 2017). Applying this NAM, we show that CRA of air pollution based on in vitro MPFs better predict the lung cancer risk associated with exposure to ambient air PM than currently accepted component-based approaches (Dreij et al 2017). In addition, MPFs for different Standard Reference Materials (SRMs) have been validated against Salmonella mutagenicity and in vivo carcinogenicity data (de Oliveira Galvao et al 2022). We are now applying this NAM with samples representing different emission sources and environments. This project is funded by the Swedish Research Council, STINT/CAPES, Swedish Cancer and Allergy fund and Karolinska Institutet.
Assessment of acute respiratory and cardiovascular health effects due to biomass smoke exposure in the Brazilian Amazon
Biomass burning is a global concern in terms of climate change, biological diversity, and health hazards. Globally, ca 3 billion people are exposed to biomass particulate matter (PM). Biomarkers of exposure and effect are fundamental for understanding environmental exposures, mechanistic pathways, and monitoring early adverse outcomes. Our aim is to assess the acute cardiopulmonary health effects due to short-term exposure to biomass PM among the population in Amazon deforestation arc together with in vitro analyses of sampled PM in a lung cell model to provide a mechanistic characterization of the health hazard. The project is based on a panel study in Amazon, with measurements of cardiopulmonary health and effect biomarkers using an exposure lag of 1-7 days during the wet and dry season with exposure assessment based on personal PM monitoring and urinary metabolites. The mode of action is assessed by in vitro studies using sampled PM. The project addresses the so far neglected and less understood issue of acute cardiopulmonary effects due to short-term exposure to PM from wildfires. This project will be the first of its kind to combine personal monitoring of exposure, specific cardiopulmonary biomarkers, and functional tests with mechanistic in vitro studies of collected PM in Amazon. How does this translate to public awareness? To reduce the gap between high-quality research and effectively engaging affected communities we will use environmental education strategies among the exposed population. This project is funded by the Swedish Research Council for Sustainable Development (FORMAS) and Karolinska Institutet (KI Research Foundation Grants).
Publications
Reactive oxygen species-dependent transient induction of genotoxicity by retene in human liver HepG2 cells.
Scaramboni C, Campos MLAM, Dorta DJ, de Oliveira DP, de Medeiros SRB, de Oliveira Galvão MF, Dreij K
Toxicol In Vitro 2023 Jun;():105628
A yearlong monitoring campaign of polycyclic aromatic compounds and other air pollutants at three sites in Sweden: Source identification, in vitro toxicity and human health risk assessment.
Sadiktsis I, de Oliveira Galvão MF, Mustafa M, Toublanc M, Ünlü Endirlik B, Silvergren S, Johansson C, Dreij K
Chemosphere 2023 May;():138862
Determination of whole mixture-based potency factors for cancer risk assessment of complex environmental mixtures by in vitro testing of standard reference materials.
de Oliveira Galvão MF, Sadiktsis I, Marques Pedro T, Dreij K
Environ Int 2022 Jun;166():107345
In vitro cytotoxicity and genotoxicity of single and combined pesticides used by Bolivian farmers.
Barrón Cuenca J, de Oliveira Galvão MF, Ünlü Endirlik B, Tirado N, Dreij K.
Environ. Mol. Mutagen. 2021
Polycyclic aromatic compounds in particulate matter and indoor dust at preschools in Stockholm, Sweden: Occurrence, sources and genotoxic potential in vitro.
Lim H, Sadiktsis I, de Oliveira Galvão MF, Westerholm R, Dreij K
Sci Total Environ 2021 Feb;755(Pt 1):142709
Genotoxicity and DNA damage signaling in response to complex mixtures of PAHs in biomass burning particulate matter from cashew nut roasting.
de Oliveira Galvão MF, Sadiktsis I, Batistuzzo de Medeiros SR, Dreij K
Environ Pollut 2020 Jan;256():113381
Buccal micronucleus cytome assay: Inter-laboratory scoring exercise and micronucleus and nuclear abnormalities frequencies in different populations from Brazil.
Rohr P, da Silva GF, Vicentini VEP, Almeida IV, Dos Santos RA, Takahashi CS, Goulart MO, da Silva GN, de Oliveira LB, Grisolia CK, Piau TB, Bassi Branco CL, Reis ÉM, de Oliveira Galvão MF, de Medeiros SRB, Monteiro MS, de Vasconcelos Lopes RA, Brandão SFI, Batista NJC, Paz MFCJ, da Silva J
Toxicol Lett 2020 Oct;333():242-250
Physico-chemical properties and genotoxic effects of air particulate matter collected from a complex of ceramic industries.
Silva KK, Duarte FT, Rodrigues Matias JN, Morais MDSA, Duarte ESF, Soares CGCS, Hoelzemann JJ, de Oliveira Galvão MF. Atmospheric Pollution Research, 10, 597-607, 2019.
Oxidative stress, mutagenic effects, and cell death induced by retene.
Peixoto MS, da Silva Junior FC, de Oliveira Galvão MF, Roubicek DA, de Oliveira Alves N, Batistuzzo de Medeiros SR
Chemosphere 2019 Sep;231():518-527
TREML4 mRNA Expression and Polymorphisms in Blood Leukocytes are Associated with Atherosclerotic Lesion Extension in Coronary Artery Disease.
Duarte VHR, Miranda CTOF, Cruz MS, de Araújo JNG, Duarte MKRN, Santos AMQSD, Santos ICCD, Santos JCD, da Silva AMG, de Oliveira JM, Paiva MSMO, de Oliveira Galvão MF, Rezende AA, Hirata MH, Hirata RDC, Luchessi AD, Silbiger VN
Sci Rep 2019 05;9(1):7229
Biomass burning particles in the Brazilian Amazon region: Mutagenic effects of nitro and oxy-PAHs and assessment of health risks.
de Oliveira Galvão MF, de Oliveira Alves N, Ferreira PA, Caumo S, de Castro Vasconcellos P, Artaxo P, de Souza Hacon S, Roubicek DA, Batistuzzo de Medeiros SR
Environ Pollut 2018 Feb;233():960-970
Characterization of the particulate matter and relationship between buccal micronucleus and urinary 1-hydroxypyrene levels among cashew nut roasting workers.
de Oliveira Galvão MF, de Queiroz JDF, Duarte ESF, Hoelzemann JJ, de André PA, Saldiva PHN, Menezes Filho JA, Batistuzzo de Medeiros SR
Environ Pollut 2017 Jan;220(Pt A):659-671
Cell death pathways of particulate matter toxicity.
Peixoto MS, de Oliveira Galvão MF, Batistuzzo de Medeiros SR
Chemosphere 2017 Dec;188():32-48
Mutagenic potential assessment associated with human exposure to natural radioactivity.
Marcon AE, Navoni JA, de Oliveira Galvão MF, Garcia ACFS, do Amaral VS, Petta RA, Campos TFDC, Panosso R, Quinelato AL, de Medeiros SRB
Chemosphere 2017 Jan;167():36-43
Cashew nut roasting: chemical characterization of particulate matter and genotocixity analysis.
Galvão MF, Cabral Tde M, de André PA, Andrade Mde F, de Miranda RM, Saldiva PH, Vasconcellos Pde C, de Medeiros SR
Environ Res 2014 May;131():145-52
Genetic damage of organic matter in the Brazilian Amazon: a comparative study between intense and moderate biomass burning.
de Oliveira Alves N, de Souza Hacon S, de Oliveira Galvão MF, Simões Peixotoc M, Artaxo P, de Castro Vasconcellos P, de Medeiros SR
Environ Res 2014 Apr;130():51-8