Research description
Pluripotency, Embryonic Stem Cells and Regenerative Medicine
The Lanner lab aims to:
1. Uncover the fundamental principles underlying how the first cell types, totipotency and pluripotency is established in the early human embryo.
2. Develop embryonic stem cell based strategies. We have establish a first clinically compliant good manufacturing practice (GMP) grade human embryonic stem cell line, KARO1.
3. Treatment of Diabetes. We are actively working to establish methodology both to efficiently produce glucose responsive beta cells and evaluate the optimal way to deliver these to the patient. We are particularly interested in exploring the anterior chamber of the eye as an alternative site of transplantation.
4. Treatment of age-related macula degeneration (AMD). During collaborative agreement with St. Erik Eye Hospital and Novo Nordisk A/S we have manufactured hESC-RPE cells (CellThRPE1) according to our developed protocol and performed preclinical toxicology and functionality testing. With the support of Erling-Persson Foundation, we are now aiming to initiate clinical trial for treatment of AMD. Additional partners are welcomed to contact us. We continue to advance related methodologies and increase our fundamental knowledge of how the different cell types of the retina are established during embryogenesis and in vitro.
Current Lab members
Dr. Elisabeth Moussaud, Research Coordinator Lanner and Damdimopoulou lab
Dr. Cheng Zhao, Research Specialist shared with Sophie Petropoulos. Bioinformatics in embryo and stem cell biology.
Dr. Sanjiv Risal, Research specialist within Acharya lab. Spatial transcriptomics of embryo development.
Dr. Shivam Chandel, Postdoc. Developing insulin producing stem cell derived islets.
Dr. Mathilde Bergamelli, Postdoc shared with Andres Salumets. Using blastoids to mimic embryo implantation biology.
Dr. Jing Lyu, Postdoc performing epigenetic studies or early human embryos.
Laura Baque Vidal, PhD student. Genetic engineering to combat transplantation rejection and establishing validated release criteria for CellThRPE1.
Sara Saietz, PhD student. Embryonic stem cell-based treatment of retinal degenerative disease
Frederik Bär, PhD student. Individualized iPSC-based treatment of blindness
Past Lab members
Dr. Sophie Petroploulos, PI at KI and University of Montreal
Dr. Sebastian Hildebrand, Postdoc. Now working at Tecan
Dr. Liselotte Antonsson, Now working at Stockholm University
Dr. Sarita Panula, Postdoc. Now working at Procella Therapeutics.
Dr. Siqin Wu, Industrial Postdoc at Spiber Technologies.
Dr. Sandra Petrus-Reurer, PhD and Postdoc, now working at Cambridge University.
Dr. Sara Padrell Sánchez, PhD student now Director of Business Development at Healios
Dr. John Paul Schell. PhD and Postdoc now working in Professor Ken Chiens laboratory, KI
Dr. Paschalis Efstathopoulos, Industrial Postdoc now working as Research scientist at Novo Nordisk
Dr. Leni Wagner, PhD and Postdoc.
Dr. Alvaro Plaza Reyes, PhD and Postdoc. Currently working at CABIMER, Spain
Dr. Pankaj Kumar, Postdoc. Currently at Université libre de Bruxelles, Belgium
Dr. Nicholas Ortega, Postdoc. Currently working at Bio-Techne, Sweden
Dr. Heather Main, ATMP Sweden
Dr Jere Weltner, Postdoc. Currently at University of Helsinki
Dr. Breniere-Letuffe, Postdoc
Hazel Reilly, Currently PhD student at Karolinska Institutet, Sweden
Nerges Winblad, PhD student.
Mona Hedenskog, Previous lab manager, currently enjoying retirement.
Nefeli Eirini Beri, Previous MSc student
Hugo Metzger, Previous Erasmus student
Nashwan Jalal Markus, now working at the productive Unit Karolinska Hospital
Philipp Schenk
Angelos Gkikas
Nariman Gharari
Interested in joining the lab?
Applications for post-doc positions are very welcomed if the applicant can bring financing. Otherwise early contacts are encouraged to plan applications for external personal post-doc grants.
Publications
Complete human day 14 post-implantation embryo models from naïve ES cells
B. Oldak, E. Wildschutz, V. Bondarenko, A. Aguilera-Castrejon, C. Zhao, S. Tarazi, M-Y. Comar, S. Ashouokhi, D. Lokshtanov, F. Roncato, S. Viukov, E. Ariel, M. Rose, N. Livnat, T. Shani, C. Joubran, R. Cohen, Y. Addadi, M. Kedmi, H Keren-Shaul, V Pasque, S Petropoulos, F. Lanner, N. Novershtern, J. Hanna. Nature 2023
C.H. Chan, Y.Y. Lam, N. Wong, L. Geng, J. Zhang, V. Ahola, A. Zare, R.A. Li, F. Lanner, W. Keung, Y-F. Cheung. Communications Biology 2023
The continued absence of functional germline stem cells in adult ovaries.
Yoshihara M, Wagner M, Damdimopoulos A, Zhao C, Petropoulos S, Katayama S, Kere J, Lanner F, Damdimopoulou P. Stem Cells 2022
Molecular profiling of retinal pigment epithelial cell differentiation for therapeutic use
Petrus-Reurer S, Lederer AR, Baqué-Vidal L, Douagi I, Pannagel B, Aronsson M, Bartuma H, Wagner M, André H, Sundström E, Bhaduri A, Kriegstein A, Kvanta A, La Manno G, Lanner F. Stem Cell Reports 2022
Kumar B, Navarro C, Winblad N, Schell JP, Zhao C, Weltner J, Baque-Vidal L, Salazar Mantero, Petropoulos S, Lanner F, Elsässer SJ. Nature Cell Biology 2022
A peek into the black box of human embryology
Goedel A, Lanner F Nature 2021
Refined transcriptional blueprint of human preimplantation embryos
Weltner J and Lanner F Cell Stem Cell 2021
Amnion signals are essential for mesoderm formation in primates
Yang R, Goedel A, Kang Y, Si C, Chu C, Zheng Y, Chen Z, Gruber PJ, Xiao Y, Zhou C, Witman N, Leung CY, Chen Y, Fu J, Ji W, Lanner F, Niu Y, Chien K. Nat Commun. 2021 Browse the transcriptional atlas here
All models are wrong, but some are useful: Establishing standards for stem cell-based embryo models
Posfai E, Lanner F, Mulas C, Leitch HG. Stem Cell Reports 2021
Reprogrammed iBlastoids contain amnion-like cells but not trophectoderm
Zhao C, Plaza Reyes A, Schell JP, Weltner J, Ortega N, Zheng Y, Björklund ÅK, Rossant J, Fu J, Petropoulos S, Lanner F. BioRxiv 2021
Evaluating totipotency using criteria of increasing stringency
Posfai E, Schell JP, Janiszewski A, Rovic I, Murray A, Bradshaw B, Yamakawa T, Pardon T, El Bakkali M, Talon I, De Geest N, Kumar P, To SK, Petropoulos S, Jurisicova A, Pasque V, Lanner F and Janet Rossant. Nature Cell Biology, 2021
Plaza Reyes A, Petrus-Reurer S, Padrell Sánchez S, Kumar P, Douagi I, Bartuma H, Aronsson M, Westman S, Lardner E, André H, Falk A, Nandrot EF, Kvanta A, Lanner F. Nat Commun. 2020
Petrus-Reurer S, Kumar P, Padrell Sánchez S, Aronsson M, André H, Bartuma H, Plaza Reyes A, Nandrot EF, Kvanta A, Lanner F. Stem Cells Transl Med. 2020
Petrus-Reurer S, Winblad N, Kumar P, Gorchs L, Chrobok M, Wagner AK, Bartuma H, Lardner E, Aronsson M, Plaza Reyes Á, André H, Alici E, Kaipe H, Kvanta A, Lanner F. Stem Cell Reports. 2020
Karolinska Institutet Human Embryonic Stem Cell Bank
Main H, Hedenskog M, Acharya G, Hovatta O, Lanner F. Stem Cell Research. 2020
Wagner M, Yoshihara M, Douagi I, Damdimopoulos A, Panula S, Petropoulos S, Lu H, Pettersson K, Palm K, Katayama S, Hovatta O, Kere J, Lanner F, Damdimopoulou P.
Nat Commun. 2020. Browse the transcriptional data in our Eovary
Fernandez-Alonso R, Bustos F, Budzyk M, Kumar P, Helbig AO, Hukelmann J, Lamond AI, Lanner F, Zhou H, Petsalaki E, Findlay GM. Nat Commun. 2020
Panula S, Kurek M, Kumar P, Albalushi H, Padrell Sánchez S, Damdimopoulou P, Olofsson JI, Hovatta O, Lanner F, Stukenborg JB. Hum Reprod. 2019
iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture.
Gelali E, Girelli G, Matsumoto M, Wernersson E, Custodio J, Mota A, Schweitzer M, Ferenc K, Li X, Mirzazadeh R, Agostini F, Schell JP, Lanner F, Crosetto N, Bienko M.Nat Commun. 2019
Time Matters: Gene Editing at the Mouse 2-Cell Embryo Stage Boosts Knockin Efficiency.
Plaza Reyes A, Lanner F. Cell Stem Cell. 2018
Functional genetics of early human development.
Ortega NM, Winblad N, Plaza Reyes A, Lanner F. Curr Opin Genet Dev. 2018
Sandra Petrus-Reurer, Hammurabi Bartuma, Monica Aronsson, Sofie Westman, Fredrik Lanner, Anders Kvanta, JOVE 2018
Sebastian Hildebrand, Sara Hultin,Aravindh Subramani, Sophie Petropoulos, Yuanyuan Zhang, Xiaofang Cao, John Mpindi, Olli Kalloniemi, Staffan Johansson, Arindam Majumdar, Fredrik Lanner & Lars Holmgren. Scientific Reports 2017
At the heart of gene edits in human embryo
Winblad N., and Lanner F, Nature 2017
Authors Nosi U, Lanner F, Huang T, Cox B. Cell Reports 2017
Collier AJ, Panula SP, Schell JP, Chovanec P, Plaza Reyes A, Petropoulos S, Corcoran AE, Walker R, Douagi I, Lanner F, Rugg-Gunn PJ. Cell Stem Cell. 2017
Petrus-Reurer S, Bartuma H, Aronsson M, Westman S, Lanner F, André H, Kvanta A. Invest Ophthalmol Vis Sci. 2017 Feb 1;58(2):1314-1322. doi: 10.1167/iovs.16-20738.
Posfai E, Petropoulos S, de Barros FR, Schell JP, Jurisica I, Sandberg R, Lanner F, Rossant J. Elife. 2017 Feb 22;6. pii: e22906. doi: 10.7554/eLife.22906.
Plaza Reyes A. and Lanner F. Development 2017 144: 3-7; doi: 10.1242/dev.139683
Single-cell sequencing of the small-RNA transcriptome
Faridani OR, Abdullayev I, Hagemann-Jensen M, Schell JP, Lanner F, Sandberg R. Nat Biotechnol. 2016 Oct 31. doi: 10.1038/nbt.3701.
Single-cell analyses of X Chromosome inactivation dynamics and pluripotency during differentiation.
Chen G, Schell JP, Benitez JA, Petropoulos S, Yilmaz M, Reinius B, Alekseenko Z, Shi L, Hedlund E, Lanner F, Sandberg R, Deng Q.
Genome Res. 2016 Aug 2. pii: gr.201954.115. [Epub ahead of print]
Madissoon E, Jouhilahti EM, Vesterlund L, Töhönen V, Krjutškov K, Petropoulous S, Einarsdottir E, Linnarsson S, Lanner F, Månsson R, Hovatta O, Bürglin TR, Katayama S, Kere J.
Sci Rep. 2016 Jul 14;6:28995. doi: 10.1038/srep28995.
Single-cell RNA-seq reveals lineage and X-chromosome dynamics in human preimplantation embryos
Sophie Petropoulos, Daniel Edsgärd, Björn Reinius, Qiaolin Deng, Sarita Pauliina Panula, Simone Codeluppi, Alvaro Plaza Reyes, Sten Linnarsson, Rickard Sandberg, Fredrik Lanner
Cell, published online 7 April 2016, doi: 10.1016/j.cell.2016.03.023
Petropoulos S, Panula SP, Schell JP, Lanner F.
J Intern Med. 2016 Apr 5. doi: 10.1111/joim.12493.
Alvaro Plaza Reyes, Sandra Petrus-Reurer, Liselotte Antonsson, Sonya Stenfelt, Hammurabi Bartuma, Sarita Panula, Theresa Mader, Iyadh Douagi, Helder André, Outi Hovatta, Fredrik Lanner, Anders Kvanta.
Stem Cell Reports, online 24 december 2015
Adult human and mouse ovaries lack DDX4-expressing functional oogonial stem cells.
Zhang H, Panula S, Petropoulos S, Edsgärd D, Busayavalasa K, Liu L, Li X, Risal S, Shen Y, Shao J, Liu M, Li S, Zhang D, Zhang X, Gerner RR, Sheikhi M, Damdimopoulou P, Sandberg R, Douagi I, Gustafsson JÅ, Liu L, Lanner F, Hovatta O, Liu K.
Nat Med. 2015 Oct 7;21(10):1116-8
Lineage specification in the early mouse embryo.
Lanner F.
Exp Cell Res. 2014 Feb 1;321(1):32-9. doi: 10.1016/j.yexcr.2013.12.004
Findlay GM, Smith MJ, Lanner F, Hsiung MS, Gish GD, Petsalaki E, Cockburn K, Kaneko T, Huang H, Bagshaw RD, Ketela T, Tucholska M, Taylor L, Bowtell DD, Moffat J, Ikura M, Li SS, Sidhu SS, Rossant J, Pawson T.
Cell. 2013 Feb 28;152(5):1008-20. doi: 10.1016/j.cell.2013.01.056.
Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells.
Rugg-Gunn PJ, Cox BJ, Lanner F, Sharma P, Ignatchenko V, McDonald AC, Garner J, Gramolini AO, Rossant J, Kislinger T.
Dev Cell. 2012 Apr 17;22(4):887-901. doi: 10.1016/j.devcel.2012.01.005.
The role of FGF/Erk signaling in pluripotent cells.
Lanner F, Rossant J.
Development. 2010 Oct;137(20):3351-60. doi: 10.1242/dev.050146.
FGF signal-dependent segregation of primitive endoderm and epiblast in the mouse blastocyst.
Yamanaka Y, Lanner F, Rossant J.
Development. 2010 Mar;137(5):715-24. doi: 10.1242/dev.043471.
Academic honours, awards and prizes
Vinnova precision medicin centre IndiCell
Novo Nordisk collaborative support
Swedish Foundation for Strategic Research
Research Associate position, funded by the Board of Research, Karolinska Institutet
Knut och Alice Wallenbergs Stiftelse