Research description
Pluripotency, Embryonic Stem Cells and Regenerative Medicine
The Lanner lab aims to:
1. Uncover the fundamental principles underlying how the first cell types and pluripotency is established in the early human embryo.
2. Establish a good manufacturing practice (GMP) bank of clinically compliant high quality embryonic stem cells (ESCs).
3. Explore treatment of age-related macula degeneration using ESC derived retinal pigmented epithelial cells.
ESCs isolated from the human blastocyst can be propagated indefinitely and directed into any cell type of the adult human, offering great potential for regenerative medicine to replace lost or damaged tissues. The knowledge of how these pluripotent cells are established and controlled in vivo is generated primarily in animal modes such as the mouse while our knowledge of the same process in the human is rudimentary and key differences are emerging. We will explore these issues through single cell mRNA sequencing, pioneering genetic technologies and functional assays in human blastocysts. This fundamental knowledge will also be important for our understanding of infertility, a problem affecting 10-15% of all couples in our society.
We have established an efficient differentiation methodology of ESCs into clinically relevant cell types. However, before these safely can become clinical practice, additional hurdles must be overcome such as GMP production and immune matching of cells and patients. By establishing the KI Stem Cell Bank we hope to resolve these issues and facilitate efforts to move stem cell based regenerative medicine into the clinic. Finally, we will specifically aim to initiate trials based on ESC in treatment of macular degeneration, the major cause of blindness in our elderly population.
Current Lab members
Mona Hedenskog, Lab technician, Establishing clinically compliant embryonic stem cells
Dr. Heather Main, BMA, Establishing GMP grade retinal cells from embryonic stem cells
Dr. Sarita Pauliina Panula, Postdoc. Establishing novel tools to explore distinct stem cell types in the human embryo.
Dr. Siqin Wu, Industrial Postdoc (Spiber Technologies). Developing novel ESCs differentiation strategies using spider silk.
Dr. Pankaj Kumar, Postdoc. Bioinformatics of pluripotency and early human development.
Dr. Nicolas Ortega, Postdoc. Gene editing in early bovine and human embryos.
Dr. Paschalis Efstathopoulos, Postdoc. Generation of pancreatic tissues for treatment of diabetes
Dr. Sandra Petrus-Reurer, Postdoc also working in Anders Kvantas group. Treating AMD using ESCs.
John Paul Schell, PhD student. Dissecting naive and primed pluripotency.
Alvaro Plaza Reyes, PhD student. Treating AMD using ESCs. Establishing genetic technologies to study early human development.
Sara Padrell Sánchez, PhD student. Treating AMD using ESCs.
Leni Wagner, PhD student in Damdimopoulou Lab, co-supervised. Stem cells of the adult ovary.
Nerges Winblad, Research assistant. Establishing genetic technologies to study early human development.
Nashwan Jalal Markus, PhD student, Genetics of early human development
Laura Baque Vidal, ERASMUS student
Nariman Gharari, Master student, Pancreatic differentiation
Past Lab members
Dr. Sophie Petroploulos, PI at KI and University of Montreal
Dr. Sebastian Hildebrand, Postdoc. Now working at Tecan
Dr. Liselotte Antonsson
Philipp Schenk
Angelos Gkikas
Interested in joining the lab? Applications for post-doc positions are very welcomed if the applicant can bring financing. Otherwise early contacts are encouraged to plan applications for external personal post-doc grants.
KI Stem Cell Bank
Together with Mona Hedenskog and Heather Main we are working intensively to derive clinically compliant ESCs which we will be usefull for academic and industial efforts within stem cell based regenerative medicin. Our first GMP grade line is derived, please contact us if interested in such cell line.
Publications
iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture.
Gelali E, Girelli G, Matsumoto M, Wernersson E, Custodio J, Mota A, Schweitzer M, Ferenc K, Li X, Mirzazadeh R, Agostini F, Schell JP, Lanner F, Crosetto N, Bienko M.Nat Commun. 2019
Time Matters: Gene Editing at the Mouse 2-Cell Embryo Stage Boosts Knockin Efficiency.
Plaza Reyes A, Lanner F. Cell Stem Cell. 2018
Functional genetics of early human development.
Ortega NM, Winblad N, Plaza Reyes A, Lanner F. Curr Opin Genet Dev. 2018
Sandra Petrus-Reurer, Hammurabi Bartuma, Monica Aronsson, Sofie Westman, Fredrik Lanner, Anders Kvanta, JOVE 2018
Sebastian Hildebrand, Sara Hultin,Aravindh Subramani, Sophie Petropoulos, Yuanyuan Zhang, Xiaofang Cao, John Mpindi, Olli Kalloniemi, Staffan Johansson, Arindam Majumdar, Fredrik Lanner & Lars Holmgren. Scientific Reports 2017
At the heart of gene edits in human embryo
Winblad N., and Lanner F, Nature 2017
Authors Nosi U, Lanner F, Huang T, Cox B. Cell Reports 2017
Collier AJ, Panula SP, Schell JP, Chovanec P, Plaza Reyes A, Petropoulos S, Corcoran AE, Walker R, Douagi I, Lanner F, Rugg-Gunn PJ. Cell Stem Cell. 2017
Petrus-Reurer S, Bartuma H, Aronsson M, Westman S, Lanner F, André H, Kvanta A. Invest Ophthalmol Vis Sci. 2017 Feb 1;58(2):1314-1322. doi: 10.1167/iovs.16-20738.
Posfai E, Petropoulos S, de Barros FR, Schell JP, Jurisica I, Sandberg R, Lanner F, Rossant J. Elife. 2017 Feb 22;6. pii: e22906. doi: 10.7554/eLife.22906.
Plaza Reyes A. and Lanner F. Development 2017 144: 3-7; doi: 10.1242/dev.139683
Single-cell sequencing of the small-RNA transcriptome
Faridani OR, Abdullayev I, Hagemann-Jensen M, Schell JP, Lanner F, Sandberg R. Nat Biotechnol. 2016 Oct 31. doi: 10.1038/nbt.3701.
Single-cell analyses of X Chromosome inactivation dynamics and pluripotency during differentiation.
Chen G, Schell JP, Benitez JA, Petropoulos S, Yilmaz M, Reinius B, Alekseenko Z, Shi L, Hedlund E, Lanner F, Sandberg R, Deng Q.
Genome Res. 2016 Aug 2. pii: gr.201954.115. [Epub ahead of print]
Madissoon E, Jouhilahti EM, Vesterlund L, Töhönen V, Krjutškov K, Petropoulous S, Einarsdottir E, Linnarsson S, Lanner F, Månsson R, Hovatta O, Bürglin TR, Katayama S, Kere J.
Sci Rep. 2016 Jul 14;6:28995. doi: 10.1038/srep28995.
Single-cell RNA-seq reveals lineage and X-chromosome dynamics in human preimplantation embryos Sophie Petropoulos, Daniel Edsgärd, Björn Reinius, Qiaolin Deng, Sarita Pauliina Panula, Simone Codeluppi, Alvaro Plaza Reyes, Sten Linnarsson, Rickard Sandberg, Fredrik Lanner Cell, published online 7 April 2016, doi: 10.1016/j.cell.2016.03.023
Petropoulos S, Panula SP, Schell JP, Lanner F.
J Intern Med. 2016 Apr 5. doi: 10.1111/joim.12493.
Alvaro Plaza Reyes, Sandra Petrus-Reurer, Liselotte Antonsson, Sonya Stenfelt, Hammurabi Bartuma, Sarita Panula, Theresa Mader, Iyadh Douagi, Helder André, Outi Hovatta, Fredrik Lanner, Anders Kvanta.
Stem Cell Reports, online 24 december 2015
Adult human and mouse ovaries lack DDX4-expressing functional oogonial stem cells.
Zhang H, Panula S, Petropoulos S, Edsgärd D, Busayavalasa K, Liu L, Li X, Risal S, Shen Y, Shao J, Liu M, Li S, Zhang D, Zhang X, Gerner RR, Sheikhi M, Damdimopoulou P, Sandberg R, Douagi I, Gustafsson JÅ, Liu L, Lanner F, Hovatta O, Liu K.
Nat Med. 2015 Oct 7;21(10):1116-8
Lineage specification in the early mouse embryo.
Lanner F.
Exp Cell Res. 2014 Feb 1;321(1):32-9. doi: 10.1016/j.yexcr.2013.12.004
Findlay GM, Smith MJ, Lanner F, Hsiung MS, Gish GD, Petsalaki E, Cockburn K, Kaneko T, Huang H, Bagshaw RD, Ketela T, Tucholska M, Taylor L, Bowtell DD, Moffat J, Ikura M, Li SS, Sidhu SS, Rossant J, Pawson T.
Cell. 2013 Feb 28;152(5):1008-20. doi: 10.1016/j.cell.2013.01.056.
Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells.
Rugg-Gunn PJ, Cox BJ, Lanner F, Sharma P, Ignatchenko V, McDonald AC, Garner J, Gramolini AO, Rossant J, Kislinger T.
Dev Cell. 2012 Apr 17;22(4):887-901. doi: 10.1016/j.devcel.2012.01.005.
The role of FGF/Erk signaling in pluripotent cells.
Lanner F, Rossant J.
Development. 2010 Oct;137(20):3351-60. doi: 10.1242/dev.050146.
FGF signal-dependent segregation of primitive endoderm and epiblast in the mouse blastocyst.
Yamanaka Y, Lanner F, Rossant J.
Development. 2010 Mar;137(5):715-24. doi: 10.1242/dev.043471.
Academic honours, awards and prizes
Swedish Foundation for Strategic Research
Research Associate position, funded by the Board of Research, Karolinska Institutet