About me

Current position: Professor of Glial Cell Biology

Research group's website


Main interests: Single cell  and spatial transcriptomics and epigenomics, oligodendroglia, remyelination, multiple sclerosis, neuroimmunology

Research description

Our research group is interested in the molecular mechanisms defining the epigenetic state of oligodendrocyte lineage cells. We are particularly focused on how interplay between transcription factors, non-coding RNAs and chromatin modifying enzymes contributes to the transition between epigenetic states in oligodendrocyte precursor cells, and could thereby lead to targeted therapies for illnesses such as multiple sclerosis.

Selected publications: 

Spatially resolved epigenome-transcriptome co-profiling of mammalian tissues

Di Zhang, Yanxiang Deng, Petra Kukanja, Eneritz Agirre, Marek Bartosovic, Mingze Dong, Cong Ma, Sai Ma, Graham Su, Shuozhen Bao, Yang Liu, Yang Xiao, Gorazd B. Rosoklija, Andrew J. Dwork, J. John Mann, Kam W. Leong, Maura Boldrini, Liya Wang, Maximilian Haeussler, Benjamin J. Raphael, Yuval Kluger, Gonçalo Castelo-Branco*, Rong Fan* Nature 2023, 616, 113–122

Multimodal chromatin profiling using nanobody-based single-cell CUT&Tag. Bartosovic M, Castelo-Branco G

Nat Biotechnol 2022 Dec;():

Spatial profiling of chromatin accessibility in mouse and human tissues. Deng Y, Bartosovic M, Ma S, Zhang D, Kukanja P, Xiao Y, Su G, Liu Y, Qin X, Rosoklija GB, Dwork AJ, Mann JJ, Xu ML, Halene S, Craft JE, Leong KW, Boldrini M, Castelo-Branco G, Fan R

Nature 2022 Sep;609(7926):375-383

Spatial-CUT&Tag: Spatially resolved chromatin modification profiling at the cellular level. Deng Y, Bartosovic M, Kukanja P, Zhang D, Liu Y, Su G, Enninful A, Bai Z, Castelo-Branco G, Fan R  

Science 2022 Feb;375(6581):681-686

Epigenomic priming of immune genes implicates oligodendroglia in multiple sclerosis susceptibility Mandy Meijer*, Eneritz Agirre*, Mukund Kabbe, Cassandra A. van Tuijn, Abeer Heskol, Chao Zheng, Ana Mendanha Falcão, Marek Bartosovic, Leslie Kirby, Daniela Calini, Michael R. Johnson, M. Ryan Corces, Thomas J. Montine, Xingqi Chen, Howard Y. Chang, Dheeraj Malhotra, and Gonçalo Castelo-Branco  

Neuron 2022 doi: 10.1016/j.neuron.2021.12.034

Single-cell CUT&Tag profiles histone modifications and transcription factors in complex tissues.

Bartosovic M, Kabbe M, Castelo-Branco G

Nat Biotechnol 2021

Altered human oligodendrocyte heterogeneity in multiple sclerosis

Sarah Jäkel*, Eneritz Agirre*, Ana Mendanha Falcão, David van Bruggen, Ka Wai Lee, Irene Knuesel, Dheeraj Malhotra, Charles ffrench-Constant, Anna Williams and Gonçalo Castelo-Branco.

Nature 2019

Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis

Ana Mendanha Falcão*, David van Bruggen*, Sueli Marques, Mandy Meijer, Sarah Jäkel, Eneritz Agirre, Samudyata, Elisa M. Floriddia, Darya P. Vanichkina,  Charles ffrench-Constant, Anna Williams, André Ortlieb Guerreiro-Cacais & Gonçalo Castelo-Branco 

Nature Medicine 2018 Dec;24(12):1837-1844

Transcriptional Convergence of Oligodendrocyte Lineage Progenitors during Development.

Marques *, van BruggenD*, Vanichkina D*, Floriddia EM, Munguba H, Väremo L, Giacomello S, Falcão AM, Meijer M, Björklund ÅK, Hjerling-Leffler J, Taft RJ, Castelo-Branco G.

Developmental Cell Volume 46, Issue 4, 20 August 2018, Pages 504-517.e7

Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system

Sueli Marques†, Amit Zeisel†, Simone Codeluppi, David van Bruggen, Ana Mendanha Falcão, Lin Xiao, Huiliang Li, Martin Häring, Hannah Hochgerner, Roman A. Romanov, Daniel Gyllborg, Ana Muñoz Manchado, Gioele La Manno, Peter Lönnerberg, Elisa M. Floriddia, Fatemah Rezayee, Patrik Ernfors, Ernest Arenas, Jens Hjerling-Leffler, Tibor Harkany, William D. Richardson, Sten Linnarsson*, Gonçalo Castelo-Branco*

SCIENCE 2016 Jun;352(6291):1326-1329



Associate Professor (Docent), Karolinska Institutet, Stockholm, Sweden

Post-doctoral fellow, The Gurdon Institute, University of Cambridge, United Kingdom Kouzarides' lab

Post-doctoral fellow, Department of Neuroscience, Karolinska Institutet, Sweden, Hermanson's lab

PhD, Medical Biochemistry, Karolinska Institutet, Sweden, Arenas's lab 

B.Sc., Biochemistry, University of Coimbra, Portugal, B.Sc. student at Saraiva's lab, Institute for Molecular and Cellular Biology, University of Porto, Portugal

Academic honours, awards and prizes

2023-        Member of Nobel Assembly at Karolinska Institutet

2022          Hans Wigzell Prize 

2021           Eric K. Fernström Prize 

2021           Göran Gustafsson Prize in Medicine

2019           Swedish Society for Medical Research (SSMF) Jubileum Prize