About me
Current position: Professor of Glial Cell Biology
Main interests: Single cell and spatial transcriptomics and epigenomics, oligodendroglia, remyelination, multiple sclerosis, neuroimmunology
Research description
Our research group is interested in the molecular mechanisms defining the epigenetic state of oligodendrocyte lineage cells. We are particularly focused on how interplay between transcription factors, non-coding RNAs and chromatin modifying enzymes contributes to the transition between epigenetic states in oligodendrocyte precursor cells, and could thereby lead to targeted therapies for illnesses such as multiple sclerosis.
Selected publications:
Spatially resolved epigenome-transcriptome co-profiling of mammalian tissues
Di Zhang, Yanxiang Deng, Petra Kukanja, Eneritz Agirre, Marek Bartosovic, Mingze Dong, Cong Ma, Sai Ma, Graham Su, Shuozhen Bao, Yang Liu, Yang Xiao, Gorazd B. Rosoklija, Andrew J. Dwork, J. John Mann, Kam W. Leong, Maura Boldrini, Liya Wang, Maximilian Haeussler, Benjamin J. Raphael, Yuval Kluger, Gonçalo Castelo-Branco*, Rong Fan* Nature 2023, 616, 113–122
Multimodal chromatin profiling using nanobody-based single-cell CUT&Tag. Bartosovic M, Castelo-Branco G
Nat Biotechnol 2022 Dec;():
Spatial profiling of chromatin accessibility in mouse and human tissues. Deng Y, Bartosovic M, Ma S, Zhang D, Kukanja P, Xiao Y, Su G, Liu Y, Qin X, Rosoklija GB, Dwork AJ, Mann JJ, Xu ML, Halene S, Craft JE, Leong KW, Boldrini M, Castelo-Branco G, Fan R
Nature 2022 Sep;609(7926):375-383
Spatial-CUT&Tag: Spatially resolved chromatin modification profiling at the cellular level. Deng Y, Bartosovic M, Kukanja P, Zhang D, Liu Y, Su G, Enninful A, Bai Z, Castelo-Branco G, Fan R
Science 2022 Feb;375(6581):681-686
Epigenomic priming of immune genes implicates oligodendroglia in multiple sclerosis susceptibility Mandy Meijer*, Eneritz Agirre*, Mukund Kabbe, Cassandra A. van Tuijn, Abeer Heskol, Chao Zheng, Ana Mendanha Falcão, Marek Bartosovic, Leslie Kirby, Daniela Calini, Michael R. Johnson, M. Ryan Corces, Thomas J. Montine, Xingqi Chen, Howard Y. Chang, Dheeraj Malhotra, and Gonçalo Castelo-Branco
Neuron 2022 doi: 10.1016/j.neuron.2021.12.034
Single-cell CUT&Tag profiles histone modifications and transcription factors in complex tissues.
Bartosovic M, Kabbe M, Castelo-Branco G
Nat Biotechnol 2021
Altered human oligodendrocyte heterogeneity in multiple sclerosis
Sarah Jäkel*, Eneritz Agirre*, Ana Mendanha Falcão, David van Bruggen, Ka Wai Lee, Irene Knuesel, Dheeraj Malhotra, Charles ffrench-Constant, Anna Williams and Gonçalo Castelo-Branco.
Nature 2019
Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis
Ana Mendanha Falcão*, David van Bruggen*, Sueli Marques, Mandy Meijer, Sarah Jäkel, Eneritz Agirre, Samudyata, Elisa M. Floriddia, Darya P. Vanichkina, Charles ffrench-Constant, Anna Williams, André Ortlieb Guerreiro-Cacais & Gonçalo Castelo-Branco
Nature Medicine 2018 Dec;24(12):1837-1844
Transcriptional Convergence of Oligodendrocyte Lineage Progenitors during Development.
Marques *, van BruggenD*, Vanichkina D*, Floriddia EM, Munguba H, Väremo L, Giacomello S, Falcão AM, Meijer M, Björklund ÅK, Hjerling-Leffler J, Taft RJ, Castelo-Branco G.
Developmental Cell Volume 46, Issue 4, 20 August 2018, Pages 504-517.e7
Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system
Sueli Marques†, Amit Zeisel†, Simone Codeluppi, David van Bruggen, Ana Mendanha Falcão, Lin Xiao, Huiliang Li, Martin Häring, Hannah Hochgerner, Roman A. Romanov, Daniel Gyllborg, Ana Muñoz Manchado, Gioele La Manno, Peter Lönnerberg, Elisa M. Floriddia, Fatemah Rezayee, Patrik Ernfors, Ernest Arenas, Jens Hjerling-Leffler, Tibor Harkany, William D. Richardson, Sten Linnarsson*, Gonçalo Castelo-Branco*
SCIENCE 2016 Jun;352(6291):1326-1329
Education
Associate Professor (Docent), Karolinska Institutet, Stockholm, Sweden
Post-doctoral fellow, The Gurdon Institute, University of Cambridge, United Kingdom Kouzarides' lab
Post-doctoral fellow, Department of Neuroscience, Karolinska Institutet, Sweden, Hermanson's lab
PhD, Medical Biochemistry, Karolinska Institutet, Sweden, Arenas's lab
B.Sc., Biochemistry, University of Coimbra, Portugal, B.Sc. student at Saraiva's lab, Institute for Molecular and Cellular Biology, University of Porto, Portugal
Academic honours, awards and prizes
2023- Member of Nobel Assembly at Karolinska Institutet
2022 Hans Wigzell Prize
2021 Göran Gustafsson Prize in Medicine
2019 Swedish Society for Medical Research (SSMF) Jubileum Prize