Judy Fabienne Kool

Judy Fabienne Kool

Research assistant

Judy works as a Research Assistant at IMM, graduated with Distinction from KI's Master Programme in Toxicology in 2022, and is the co-founder of Students for Sustainable Development at KI (@ssd_ki).

Research description

Currently, Judy works at IMM's SysTox Unit as a Research Assistant for Emma Wincent and Harri Alenius under a FORMAS-funded project titled "The role of host microbiota in chemically induced metabolic disruption". She screens a series of 13 selected environmental pollutants for toxicity in wild-type zebrafish at 5 dpf. In following steps, she will establish protocols for DNA extraction, as well as EC50, EC20 and NOAEC values for all selected chemicals, which will be used to determine the effects on the microbiota. In anticipation to microbial amplicon analysis of gut tissue from exposed and non-exposed zebrafish larvae, Judy will extract microbial DNA from zebrafish gut tissue and run PCR, upon which 16s RNA sequencing will be done.

During her Master degree project, Judy investigated the effects of two groups of ubiquitous anthropogenic pollutants (PFAS and monoalkyl phthalate esters, MPEs) on developmental lipid metabolism in larval zebrafish. She quantified blood and tissue (liver, visceral) lipid levels as well as metabolic activity in 7, 9, and 11 day-old zebrafish, and investigated oxidative stress as an underlying mechaninsm by using an antioxidant precursor (NAC). She carried out this research at the Department of Environmental Toxicology (Miljötoxikologi) at EBC, Uppsala University, under the supervision of Associate Professor Maria Jönsson.

The focus of Judy's Bachelor thesis were the effects of micro– and nanoplastics on the lower digestive tract, for which she did a literature study on the role of microplastics in gastrointestinal oxidative stress.

Education

Judy recently acquired a MSc in Toxicology (120 ECTS) at Karolinska Institutet. Before this, she did a BSc in Biomedical Sciences (180 ECTS) at Maastricht University, the Netherlands, of which one Erasmus+ exchange semester in Molecular Cell Biology and Neuroscience at the Medical University of Innsbruck, Austria.