Martin Moche

Martin Moche

Senior Research Infrastructure Specialist
Telephone: +46852486843
Visiting address: Solnavägen 9, 17177 Stockholm
Postal address: C2 Medicinsk biokemi och biofysik, C2 Chemical Biology and Genome engineering PSF, 171 77 Stockholm
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About me

  • I am in charge of the Macromolecular X-ray crystallography activities at
    Protein Science Facility, Karolinska Institutet
    I am the Macromolecular X-ray Crystallography (MX) manager at Protein Science
    Facility [1], Karolinska Institutet in daily use by around 20 researchers.
    I am organizing the local structural biology community to share synchrotron
    beamtime, crystallization robot hardware and remotely accessible diffraction
    data analysis software to achieve common goals and stay competitive in an
    international environment.
    I am coordinating three synchrotron “block allocation groups” at Diamond
    Light Source in Oxfordshire and MAX IV in Lund in benefit of seventeen (17)
    research groups at KI, SU, UU, KTH and LiU resulting in shared beamtime for
    Macromolecular X-ray diffraction experiments.
    I am also part of a team developing a High Performance Computing
    Macromolecular X-ray crystallography (HPC-MX) software installation at NSC
    Triolith, LUNARC Aurora and the MAX IV cluster called PReSTO [2], remotely
    accessible from any pc, mac or Linux computer with internet access.
    2003-2005 postdoc with prof. Pär Nordlund at Stockholm University
    1997-2003 Ph.D. studies with prof. Ylva Lindqvist at Karolinska Institutet
    [3]
    1991-1997 M.Sc. degree in Molecular Biophysics at Umeå University
    [1] https://ki.se/en/mbb/psf-mx
    [2] https://www.nsc.liu.se/support/presto/
    [3] https://openarchive.ki.se/xmlui/handle/10616/38577

Research

  • I am happy to participate in research activities in support of
    students/postdocs/PIs with an interest in applying macromolecular X-ray
    crystallography to their research. I was the assistant supervisor of
    Madhanagopal Anandapadamanaban (1-3) that defended his thesis in December
    2015 [1]. I have also supervised postdocs in single projects without being
    an official supervisor (4-5).
    1. High-resolution structure of TBP with TAF1 reveals anchoring patterns in
    transcriptional regulation. [2]
    Anandapadamanaban M, Andresen C, Helander S, Ohyama Y, Siponen MI, Lundström
    P, Kokubo T, Ikura M, Moche M, Sunnerhagen M
    /Nat Struct Mol Biol 2013 Aug
  • 20(8):1008-14/
    2. Mutation-Induced Population Shift in the MexR Conformational Ensemble
    Disengages DNA Binding: A Novel Mechanism for MarR Family Derepression. [3]
    Anandapadamanaban M, Pilstål R, Andresen C, Trewhella J, Moche M, Wallner B,
    Sunnerhagen M
    /Structure 2016 08
  • 24(8):1311-1321/
    3. E3 ubiquitin-protein ligase TRIM21-mediated lysine capture by UBE2E1
    reveals substrate-targeting mode of a ubiquitin-conjugating E2. [4]
    Anandapadamanaban M, Kyriakidis NC, Csizmók V, Wallenhammar A, Espinosa AC,
    Ahlner A, Round AR, Trewhella J, Moche M, Wahren-Herlenius M, Sunnerhagen M
    /J Biol Chem 2019 07
  • 294(30):11404-11419/
    4. Enzymatic production of 'monoclonal stoichiometric' single-stranded DNA
    oligonucleotides. [5]
    Ducani C, Kaul C, Moche M, Shih WM, Högberg B
    /Nat Methods 2013 Jul
  • 10(7):647-52/
    5. Combined x-ray crystallography and computational modeling approach to
    investigate the Hsp90 C-terminal peptide binding to FKBP51. [6]
    Kumar R, Moche M, Winblad B, Pavlov PF
    /Sci Rep 2017 10
  • 7(1):14288/
    [1] http://liu.diva-portal.org/smash/record.jsf?pid=diva2%3A866671&
  • amp
  • dswid=-3807
    [2] https://pubmed-ncbi-nlm-nih-gov.proxy.kib.ki.se/23851461
    [3] https://pubmed-ncbi-nlm-nih-gov.proxy.kib.ki.se/27427478
    [4] https://pubmed-ncbi-nlm-nih-gov.proxy.kib.ki.se/31160341
    [5] https://pubmed-ncbi-nlm-nih-gov.proxy.kib.ki.se/23727986
    [6] https://pubmed-ncbi-nlm-nih-gov.proxy.kib.ki.se/29079741

Teaching

  • I am ususally teaching first and second year medical and biomedical students
    in fundamental concepts regarding
    protein, lipid and carbohydrate structure and function
    vitamins and cofactors
    energy metabolism such as glucolysis, TCA cycle and oxidative phosphorylation
    adding impact of diabetes on insulin/glucagon regulation
    on gluconeogenesis, glycogenolysis and glycogen synthesis
    chemical binding in organic chemistry

Articles

All other publications

Grants

Employments

  • Senior Research Infrastructure Specialist, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 2023-
  • Senior Lab Manager, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 2013-2023
  • Application Expert, Structural biology software, National Supercomputer Centre, Linköping University, 2016-2018

Degrees and Education

  • Doctor Of Philosophy, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 2003

Leadership and responsibility assignments

  • SNIC coordinator at Karolinska Institutet, Connect Life Science to High Performance Compute environments, Medical Biochemistry and Biophysics, Karolinska Institute, https://staff.ki.se/national-academic-infrastructure-for-supercomputing-in-sweden-naiss, 2019-2021

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