Oliver Mortusewicz

Oliver Mortusewicz

Principal Researcher
Visiting address: Scilifelab, Alpha Building Level 4, Tomtebodavägen 23a, 17165 Solna
Postal address: K7 Onkologi-Patologi, K7 Forskning Helleday Mortusewicz, 171 77 Stockholm
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About me

  • Principal Researcher, Team leader and Project leader at the Department of Oncology-Pathology & SciLifeLab

Research

  • A deep understanding of the molecular mechanisms of DNA repair and nucleotide metabolism is the cornerstone of our drug discovery approach.
    To be able to efficiently and successfully target proteins involved in these pathways for the treatment of cancer and other diseases, we need a detailed view of their biological functions and interactions in live cells.

    We have the unique ability to use small molecule Inhibitors and Activators developed by our excellent medicinal chemistry team as probes in our advanced molecular and cell biology assays to uncover new important biology.

    We regularly employ advanced live cell confocal microscopy in combination with laser microirradiation, Fluorescence recovery after photobleaching (FRAP) and Fluorescence loss in photobleaching (FLIP) to visualize high spatio-temporal details and highly coordinated processes of DNA repair, cell division and genome duplication in live cells. This is complemented by traditional antibody-based immunofluorescence assays in tumors and cells, allowing visualization of endogenous protein distribution and post-translational modifications after perturbation with drugs. Besides high-resolution confocal microscopy we use plate-reader microscopes for quantitative image based cytometry (QIBC), enabling us to test many different experimental settings and perturbations on a high number of cells.

Teaching

  • • Co-Supervison of four PhD Students to date
    • Supervision of undergraduate & MSc thesis projects

Articles

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