Qing Cheng

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Articles

Article: REDOX BIOCHEM CHEM. 2023;5-6:100015

Arsenic exposure and increased C-reactive protein are independently associated with lower erythrocyte glutathione peroxidase activity in Bangladeshi children.

Cheff DM; Skröder H; Akhtar E; Cheng Q; Hall MD; Raqib R; Kippler M; Vahter M; Arnér ESJ
Article: REDOX BIOLOGY. 2023;67:102897

TXNL1 has dual functions as a redox active thioredoxin-like protein as well as an ATP- and redox-independent chaperone.

Andor A; Mohanraj M; Pató ZA; Úri K; Biri-Kovács B; Cheng Q; Arnér ESJ
Article: REDOX BIOLOGY. 2023;64:102806

Cardiolipin drives the catalytic activity of GPX4 on membranes: Insights from the R152H mutant.

Roveri A; Di Giacinto F; Rossetto M; Cozza G; Cheng Q; Miotto G; Zennaro L; Di Paolo ML; Arnér ESJ; De Spirito M; Maiorino M; Ursini F
Article: REDOX BIOLOGY. 2023;63:102719

Development of an assay pipeline for the discovery of novel small molecule inhibitors of human glutathione peroxidases GPX1 and GPX4.

Cheff DM; Cheng Q; Guo H; Travers J; Klumpp-Thomas C; Shen M; Arnér ESJ; Hall MD
Article: REDOX BIOLOGY. 2023;62:102703

The ferroptosis inducing compounds RSL3 and ML162 are not direct inhibitors of GPX4 but of TXNRD1.

Cheff DM; Huang C; Scholzen KC; Gencheva R; Ronzetti MH; Cheng Q; Hall MD; Arnér ESJ
Article: REDOX BIOLOGY. 2023;59:102593

Side-by-side comparison of recombinant human glutathione peroxidases identifies overlapping substrate specificities for soluble hydroperoxides.

Schwarz M; Löser A; Cheng Q; Wichmann-Costaganna M; Schädel P; Werz O; Arnér ES; Kipp AP
Article: FRONTIERS IN MICROBIOLOGY. 2023;14:1198473

Novel gold(III)-dithiocarbamate complex targeting bacterial thioredoxin reductase: antimicrobial activity, synergy, toxicity, and mechanistic insights.

Ratia C; Ballén V; Gabasa Y; Soengas RG; Velasco-de Andrés M; Iglesias MJ; Cheng Q; Lozano F; Arnér ESJ; López-Ortiz F; Soto SM
Journal article: FREE RADICAL BIOLOGY AND MEDICINE. 2022;189:32-33

A mechanistic dissection of HyPer7, an ultrasensitive pH-independent biosensor

Daria E; Ekaterina B; Gerardo F-S; Qing C; Elias A; Joris M
Article: ELIFE. 2022;11:e74549

Redox regulation of PTPN22 affects the severity of T-cell-dependent autoimmune inflammation.

James J; Chen Y; Hernandez CM; Forster F; Dagnell M; Cheng Q; Saei AA; Gharibi H; Lahore GF; Åstrand A; Malhotra R; Malissen B; Zubarev RA; Arnér ESJ; Holmdahl R
Article: CHEM. 2022;8(5):1493-1517

Selective cellular probes for mammalian thioredoxin reductase TrxR1: rational design of RX1, a modular 1,2-thiaselenane redox probe.

Zeisel L; Felber JG; Scholzen KC; Poczka L; Cheff D; Maier MS; Cheng Q; Shen M; Hall MD; Arnér ESJ; Thorn-Seshold J; Thorn-Seshold O
Article: REDOX BIOLOGY. 2022;51:102278

Biochemical and structural characterizations of thioredoxin reductase selenoproteins of the parasitic filarial nematodes Brugia malayi and Onchocerca volvulus.

Fata F; Gencheva R; Cheng Q; Lullo R; Ardini M; Silvestri I; Gabriele F; Ippoliti R; Bulman CA; Sakanari JA; Williams DL; Arnér ESJ; Angelucci F
Article: RECOMBINANT PROTEIN EXPRESSION: PROKARYOTIC HOSTS AND CELL-FREE SYSTEMS. 2022;662:95-118

Expressing recombinant selenoproteins using redefinition of a single UAG codon in an RF1-depleted E. coli host strain.

Cheng Q; Arnér ESJ
Article: REDOX BIOLOGY. 2021;48:102184

Comprehensive chemical proteomics analyses reveal that the new TRi-1 and TRi-2 compounds are more specific thioredoxin reductase 1 inhibitors than auranofin.

Sabatier P; Beusch CM; Gencheva R; Cheng Q; Zubarev R; Arnér ESJ
Article: REDOX BIOLOGY. 2021;46:102070

Production and purification of homogenous recombinant human selenoproteins reveals a unique codon skipping event in E. coli and GPX4-specific affinity to bromosulfophthalein.

Cheng Q; Roveri A; Cozza G; Bordin L; Rohn I; Schwerdtle T; Kipp A; Ursini F; Maiorino M; Miotto G; Arnér ESJ
Article: NATURE COMMUNICATIONS. 2021;12(1):1296

System-wide identification and prioritization of enzyme substrates by thermal analysis.

Saei AA; Beusch CM; Sabatier P; Wells JA; Gharibi H; Meng Z; Chernobrovkin A; Rodin S; Näreoja K; Thorsell A-G; Karlberg T; Cheng Q; Lundström SL; Gaetani M; Végvári Á; Arnér ESJ; Schüler H; Zubarev RA
Article: ANTIOXIDANTS. 2021;10(1):111

Qualitative Differences in Protection of PTP1B Activity by the Reductive Trx1 or TRP14 Enzyme Systems upon Oxidative Challenges with Polysulfides or H2O2 Together with Bicarbonate.

Dagnell M; Cheng Q; Arnér ESJ
Article: ACS INFECTIOUS DISEASES. 2020;6(3):393-405

Characterization of Lead Compounds Targeting the Selenoprotein Thioredoxin Glutathione Reductase for Treatment of Schistosomiasis.

Lyu H; Petukhov PA; Banta PR; Jadhav A; Lea WA; Cheng Q; Arnér ESJ; Simeonov A; Thatcher GRJ; Angelucci F; Williams DL
Article: INORGANIC CHEMISTRY. 2020;59(5):2711-2718

Direct Observation of Methylmercury and Auranofin Binding to Selenocysteine in Thioredoxin Reductase.

Pickering IJ; Cheng Q; Rengifo EM; Nehzati S; Dolgova NV; Kroll T; Sokaras D; George GN; Arnér ESJ
Article: SCIENCE ADVANCES. 2020;6(1):eaax8358

Control of protein function through oxidation and reduction of persulfidated states.

Dóka É; Ida T; Dagnell M; Abiko Y; Luong NC; Balog N; Takata T; Espinosa B; Nishimura A; Cheng Q; Funato Y; Miki H; Fukuto JM; Prigge JR; Schmidt EE; Arnér ESJ; Kumagai Y; Akaike T; Nagy P
Article: REDOX BIOLOGY. 2020;28:101335

Inhibition and crosslinking of the selenoprotein thioredoxin reductase-1 by p-benzoquinone.

Shu N; Cheng Q; Arnér ESJ; Davies MJ
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2019;294(33):12330-12338

Bicarbonate is essential for protein-tyrosine phosphatase 1B (PTP1B) oxidation and cellular signaling through EGF-triggered phosphorylation cascades.

Dagnell M; Cheng Q; Rizvi SHM; Pace PE; Boivin B; Winterbourn CC; Arnér ESJ
Article: SCIENCE TRANSLATIONAL MEDICINE. 2018;10(428):eaaf7444

Irreversible inhibition of cytosolic thioredoxin reductase 1 as a mechanistic basis for anticancer therapy.

Stafford WC; Peng X; Olofsson MH; Zhang X; Luci DK; Lu L; Cheng Q; Trésaugues L; Dexheimer TS; Coussens NP; Augsten M; Ahlzén H-SM; Orwar O; Östman A; Stone-Elander S; Maloney DJ; Jadhav A; Simeonov A; Linder S; Arnér ESJ
Article: METHODS IN MOLECULAR BIOLOGY. 2018;1661:231-240

Overexpression of Recombinant Selenoproteins in E. coli.

Cheng Q; Arnér ESJ
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2017;292(35):14371-14380

Thioredoxin reductase 1 and NADPH directly protect protein tyrosine phosphatase 1B from inactivation during H2O2 exposure.

Dagnell M; Pace PE; Cheng Q; Frijhoff J; Östman A; Arnér ESJ; Hampton MB; Winterbourn CC
Article: FREE RADICAL BIOLOGY AND MEDICINE. 2017;106:270-277

Homozygous mutation in TXNRD1 is associated with genetic generalized epilepsy.

Kudin AP; Baron G; Zsurka G; Hampel KG; Elger CE; Grote A; Weber Y; Lerche H; Thiele H; Nürnberg P; Schulz H; Ruppert A-K; Sander T; Cheng Q; Arnér ES; Schomburg L; Seeher S; Fradejas-Villar N; Schweizer U; Kunz WS
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2017;292(13):5476-5487

Selenocysteine Insertion at a Predefined UAG Codon in a Release Factor 1 (RF1)-depleted Escherichia coli Host Strain Bypasses Species Barriers in Recombinant Selenoprotein Translation.

Cheng Q; Arnér ESJ
Article: EJNMMI RESEARCH. 2016;6(1):58

Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake.

Cheng Q; Wållberg H; Grafström J; Lu L; Thorell J-O; Hägg Olofsson M; Linder S; Johansson K; Tegnebratt T; Arnér ESJ; Stone-Elander S; Ahlzén H-SM; Ståhl S; Sel-tag imaging project
Article: FREE RADICAL BIOLOGY AND MEDICINE. 2016;99:426-435

Serum thioredoxin reductase is highly increased in mice with hepatocellular carcinoma and its activity is restrained by several mechanisms.

Zhang L; Cheng Q; Zhang L; Wang Y; Merrill GF; Ilani T; Fass D; Arnér ESJ; Zhang J
Article: ONCOTARGET. 2016;7(26):40233-40251

Indolin-2-one compounds targeting thioredoxin reductase as potential anticancer drug leads.

Kaminska KK; Bertrand HC; Tajima H; Stafford WC; Cheng Q; Chen W; Wells G; Arner ESJ; Chew E-H
Article: FREE RADICAL BIOLOGY AND MEDICINE. 2016;94:110-120

Details in the catalytic mechanism of mammalian thioredoxin reductase 1 revealed using point mutations and juglone-coupled enzyme activities.

Xu J; Cheng Q; Arnér ESJ
Article: SCIENCE ADVANCES. 2016;2(1):e1500968

A novel persulfide detection method reveals protein persulfide- and polysulfide-reducing functions of thioredoxin and glutathione systems.

Dóka É; Pader I; Bíró A; Johansson K; Cheng Q; Ballagó K; Prigge JR; Pastor-Flores D; Dick TP; Schmidt EE; Arnér ESJ; Nagy P
Article: CELL DEATH & DISEASE. 2015;6(1):e1616

The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death.

Xu J; Eriksson SE; Cebula M; Sandalova T; Hedström E; Pader I; Cheng Q; Myers CR; Antholine WE; Nagy P; Hellman U; Selivanova G; Lindqvist Y; Arnér ESJ
Article: NUCLEIC ACIDS RESEARCH. 2013;41(21):9800-9811

Wobble decoding by the Escherichia coli selenocysteine insertion machinery.

Xu J; Croitoru V; Rutishauser D; Cheng Q; Arnér ESJ
Article: FREE RADICAL BIOLOGY AND MEDICINE. 2013;60:183-194

Redox activation of Fe(III)-thiosemicarbazones and Fe(III)-bleomycin by thioredoxin reductase: specificity of enzymatic redox centers and analysis of reactive species formation by ESR spin trapping.

Myers JM; Cheng Q; Antholine WE; Kalyanaraman B; Filipovska A; Arnér ESJ; Myers CR
Article: BIOCHIMICA ET BIOPHYSICA ACTA: INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND BIOPHYSICS. 2013;1830(3):2562-2573

Site-specifically 11C-labeled Sel-tagged annexin A5 and a size-matched control for dynamic in vivo PET imaging of protein distribution in tissues prior to and after induced cell death.

Cheng Q; Lu L; Grafström J; Olofsson MH; Thorell J-O; Samén E; Johansson K; Ahlzén H-S; Linder S; Arnér ESJ; Stone-Elander S
Article: JOURNAL OF NUCLEAR MEDICINE. 2012;53(9):1446-1453

HER2-positive tumors imaged within 1 hour using a site-specifically 11C-labeled Sel-tagged affibody molecule.

Wållberg H; Grafström J; Cheng Q; Lu L; Martinsson Ahlzén H-S; Samén E; Thorell J-O; Johansson K; Dunås F; Olofsson MH; Stone-Elander S; Arnér ESJ; Ståhl S
Article: PLOS ONE. 2012;7(8):e42151

Combining [11C]-AnxA5 PET imaging with serum biomarkers for improved detection in live mice of modest cell death in human solid tumor xenografts.

Cheng Q; Lu L; Grafström J; Olofsson MH; Thorell J-O; Samén E; Johansson K; Ahlzén H-S; Stone-Elander S; Linder S; Arnér ESJ; Sel-tag Imaging Project
Article: FREE RADICAL BIOLOGY AND MEDICINE. 2011;50(9):1114-1123

Inhibition of thioredoxin reductase 1 by porphyrins and other small molecules identified by a high-throughput screening assay.

Prast-Nielsen S; Dexheimer TS; Schultz L; Stafford WC; Cheng Q; Xu J; Jadhav A; Arnér ESJ; Simeonov A
Article: FREE RADICAL BIOLOGY AND MEDICINE. 2011;50(6):689-699

Substrate and inhibitor specificities differ between human cytosolic and mitochondrial thioredoxin reductases: Implications for development of specific inhibitors.

Rackham O; Shearwood A-MJ; Thyer R; McNamara E; Davies SMK; Callus BA; Miranda-Vizuete A; Berners-Price SJ; Cheng Q; Arnér ESJ; Filipovska A
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2011;108(3):1064-1069

Selenoprotein TRXR-1 and GSR-1 are essential for removal of old cuticle during molting in Caenorhabditis elegans.

Stenvall J; Fierro-González JC; Swoboda P; Saamarthy K; Cheng Q; Cacho-Valadez B; Arnér ESJ; Persson OP; Miranda-Vizuete A; Tuck S
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2010;285(28):21708-21723

The selenium-independent inherent pro-oxidant NADPH oxidase activity of mammalian thioredoxin reductase and its selenium-dependent direct peroxidase activities.

Cheng Q; Antholine WE; Myers JM; Kalyanaraman B; Arnér ESJ; Myers CR
Article: ALLERGY. 2009;64(6):919-926

Prolonged antigen-exposure with carbohydrate particle based vaccination prevents allergic immune responses in sensitized mice.

Thunberg S; Neimert-Andersson T; Cheng Q; Wermeling F; Bergström U; Swedin L; Dahlén S-E; Arnér E; Scheynius A; Karlsson MCI; Gafvelin G; van Hage M; Grönlund H
Article: FREE RADICAL BIOLOGY AND MEDICINE. 2009;46(7):893-904

Highly active dimeric and low-activity tetrameric forms of selenium-containing rat thioredoxin reductase 1.

Rengby O; Cheng Q; Vahter M; Jörnvall H; Arnér ESJ
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2009;284(6):3998-4008

Crystal structure and catalysis of the selenoprotein thioredoxin reductase 1.

Cheng Q; Sandalova T; Lindqvist Y; Arnér ESJ
Article: CHEMBIOCHEM. 2006;7(12):1976-1981

Selenolthiol and dithiol C-terminal tetrapeptide motifs for one-step purification and labeling of recombinant proteins produced in E. coli.

Cheng Q; Johansson L; Thorell J-O; Fredriksson A; Samén E; Stone-Elander S; Arnér ESJ
Article: NATURE PROTOCOLS. 2006;1(2):604-613

Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with 11C.

Cheng Q; Stone-Elander S; Arnér ESJ
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Conference publication: FREE RADICAL BIOLOGY AND MEDICINE. 2023;201:39

THE ANTICANCER COMPOUNDS AURANOFIN, TRI-1 AND TRI-2 HAVE DISTINCT CYTOTOXICITY PROFILES WITH REGARDS TO THIOREDOXIN REDUCTASE INHIBITION

Gencheva R; Cheng Q; Arner E
Conference publication: FREE RADICAL BIOLOGY AND MEDICINE. 2023;201:15

TRP14 IS THE CELLULAR CYSTINE REDUCTASE AND ALSO REDUCES CYSTEINYLATED PROTEINS

Marti-Andres P; Isabela; Perez S; Rius-Perez S; Torres-Cuevas I; Colino-Lage H; Guerrero-Gomez D; Morato E; Marina A; Michalska P; Leon R; Cheng Q; Miranda-Vizuete A; Schmidt EE; Martinez-Ruiz A; Arner ESJ; Sastre J
Conference publication: FREE RADICAL BIOLOGY AND MEDICINE. 2023;201:37-38

IS THERE A SUBSTRATE SPECIFICITY OF GLUTATHIONE PEROXIDASE ISOFORMS?

Schwarz M; Cheng Q; Loeser A; Arner ESJ; Kipp AP
Preprint: 2023;bioRxiv

Ancient loss of catalytic selenocysteine spurred convergent adaptation in a mammalian oxidoreductase

Rees J; Sarangi G; Cheng Q; Floor M; Andrés A; Miguel BO; Villà-Freixa J; Arnér ESJ; Castellano S
Conference publication: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. 2023;52(SUPPL 1):S50

Probing Toxic Metals and Metalloids in Redox Biology using Synchrotron X-rays

George G; Pickering I; Rengifo EM; Dolgova N; Cheng Q; Arner E
Review: FREE RADICAL BIOLOGY AND MEDICINE. 2022;190:320-338

Thioredoxin reductase selenoproteins from different organisms as potential drug targets for treatment of human diseases.

Gencheva R; Cheng Q; Arnér ESJ
Preprint: 2022;ChemRxiv

Selective cellular probes for mammalian thioredoxin reductase TrxR1: rational design of RX1, a modular 1,2-thiaselenane redox probe

Zeisel L; Felber JG; Poczka L; Scholzen K; Cheff D; Maier MS; Cheng Q; Shen M; Hall MD; Arnér ESJ; Thorn-Seshold J; Thorn-Seshold O
Conference publication: FREE RADICAL BIOLOGY AND MEDICINE. 2022;180:68

Development of a chemical probe that selectively images the activity of mammalian Thioredoxin Reductase 1

Zeisel L; Felber J; Poczka L; Scholzen K; Maier M; Cheng Q; Arner E; Thorn-Seshold J; Thorn-Seshold O
Preprint: 2021;Research Square

Ultralight ultrafast enzymes

Zhang X; Meng Z; Beusch C; Gharibi H; Cheng Q; Stefano L; Wang J; Saei A; Vegvari A; Gaetani M; Zubarev R
Preprint: 2021;bioRxiv

Redox regulation of PTPN22 affects the severity of T cell dependent autoimmune inflammation

James J; Chen Y; Hernandez C; Forster F; Dagnell M; Cheng Q; Saei A; Gharibi H; Lahore GF; Åstrand A; Malhotra R; Malissen B; Zubarev R; Arnér ESJ; Holmdahl R
Preprint: 2021;ChemRxiv

Selective cellular probes for mammalian thioredoxin reductase TrxR1: rational design of RX1, a modular 1,2-thiaselenane redox probe

Zeisel L; Felber JG; Poczka L; Scholzen K; Maier MS; Cheng Q; Arnér ESJ; Thorn-Seshold J; Thorn-Seshold O
Preprint: 2021;ChemRxiv

Selective cellular probes for mammalian thioredoxin reductase TrxR1: rational design of RX1, a modular 1,2-thiaselenane redox probe

Zeisel L; Felber JG; Poczka L; Scholzen K; Maier MS; Cheng Q; Arnér ESJ; Thorn-Seshold J; Thorn-Seshold O
Preprint: 2021;Research Square

SIESTA as a universal unbiased proteomics approach for identification and prioritization of enzyme substrates

Saei AA; Beusch C; Sabatier P; Wells JA; Gharibi H; Meng Z; Chernobrovkin A; Rodin S; Näreoja K; Thorsell A-G; Karlberg T; Cheng Q; Lundström S; Gaetani M; Végvári Á; Arnér ESJ; Schüler H; Zubarev R
Conference publication: FREE RADICAL BIOLOGY AND MEDICINE. 2019;145:S21

Targeting of the Selenoprotein Thioredoxin Reductase-1 by p-benzoquinone

Shu N; Amer E; Cheng Q; Davies M
Book chapter: 2019;p. 153-154

New developments in recombinant selenoprotein production

Cheng Q; Arnér ESJ
Published paper: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. 2018;1862(11):2511-2517

Efficient selenocysteine-dependent reduction of toxoflavin by mammalian thioredoxin reductase.

Gencheva R; Cheng Q; Arnér ESJ
Preprint: 2018;bioRxiv

System-wide identification and prioritization of enzyme substrates by thermal analysis (SIESTA)

Saei AA; Beusch C; Sabatier P; Wells JA; Chernobrovkin A; Rodin S; Näreoja K; Thorsell A-G; Karlberg T; Cheng Q; Lundström S; Gaetani M; Végvári Á; Arnér ESJ; Schüler H; Zubarev R
Conference publication: FREE RADICAL BIOLOGY AND MEDICINE. 2018;120:S46

Screening for novel inhibitors of glutathione peroxidase

Cheff DM; Cheng Q; Hall MD; Arner E
Conference publication: FREE RADICAL BIOLOGY AND MEDICINE. 2018;120:S36

Bicarbonate is required for hydrogen peroxide-dependent inactivation of PTP1B in presence of the Trx system and peroxiredoxin

Dagnell M; Cheng Q; Pace PE; Hampton MB; Winterbourn CC; Arner ESJ
Conference publication: FREE RADICAL BIOLOGY AND MEDICINE. 2016;100:S47

Thioredoxin Reductase 1 Directly Protects Protein Tyrosine Phosphatase 1B from Inactivation by H₂O₂

Dagnell M; Pace P; Cheng Q; Frijhoff J; Ostman A; Arner E; Hampton M; Winterbourn C
Meeting abstract: FREE RADICAL BIOLOGY AND MEDICINE. 2010;49:S98-S99

The Interactions of Thioredoxin Reductase with Quinones and Acrolein: Covalent Adducts and Stimulation of NADPH Oxidase Activity

Myers CR; Cheng Q; Myers JM; Antholine WE; Arner ESJ
Conference publication: JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS. 2005;48:S38

EVALUATION OF NOVEL SELENOLTHIOL AND DITHIOL SEL-TAG VARIANTS FOR PURIFICATION AND ¹¹C-LABELING OF RECOMBINANT PROTEINS PRODUCED IN E. COLI

Cheng Q; Johansson L; Fredriksson A; Thorell J-O; Stone-Elander S; Arner ESJ
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Employments

Senior Lab Manager, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 2015-

Degrees and Education

Degree Of Doctor Of Philosophy, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 2010

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