Postdoctoral researcher with the focus on Transfusion and Regenerative medicine.
Platelet transfusion | Platelet storage lesions | Intracellular signaling | Leukemia
My current research focuses on understanding the Platelet function in Transfusion medicine.
Project 1: Therapeutic storage of platelet concentrate is a challenging problem for Transfusion Medicine.
In numerous different clinical conditions, (e.g., upon accidental injury, the treatment of many diseases and in surgery) patients may need transfusion of platelets from healthy blood donors. The platelet concentrates (PC) are purified by different techniques and stored for maximally 5 or 7 days at 22°C temperature. The quality of PC is profoundly affected by the collection, processing methods and ex vivo manipulation (such as pathogen reduction). Similarly, stored platelets undergo deleterious changes, referred to as platelet storage lesions (PSLs), which may prevent transfused platelets to respond to agonists and arrest bleeding after transfusion. However, no reliable techniques are today available to evaluate this. In this project, the aim is to study the activation and function of stored platelet concentrate using flow cytometry, a technique where unique protocols allow to evaluate important platelet subpopulations and their involvement in platelet adhesion, activation, and aggregation upon stimulation with different platelet receptor agonists. The long-term goal is to dissect platelet function during blood bank storage: towards refined platelet production for optimal transfusion outcome.
Project 2: Study different phosphorylated signalling molecules and their role at activated state of platelet upon stimulation with different agonists.
Protein phosphorylation is an important cellular regulatory mechanism. Kinase/phosphatase activity plays crucial role in phosphorylation of platelet protein to maintain the platelet functional responses. Phosphorylation of different proteins can be related to higher activation state of platelet in both pathogens treated (INTERCEPT treated) and non-treated platelet concentrates based on stimulation with different agonists. In this study, the aim is to investigate the phosphorylated levels of different signalling molecules upon stimulation of platelet in random units (both pathogens treated and non-treated) of platelet concentrates. Outcome from this research will be helpful to understand different signalling molecules association in platelet activation in response to different agonists, and their relation to distinct cellular response (e.g., reactive oxygen species and, or autophagy).
Project 3: Study the changes in platelets function and membrane receptors in patients with chronic myelomonocytic leukemia (CMML).
Chronic myelomonocytic leukaemia (CMML) is characterised by increased levels of monocyte white blood cells in peripheral blood and bone marrow. Platelet dysfunction is commonly acquired due to medications, procedures, medical conditions, and underlying hematologic diseases. Platelet expresses different receptors which are important for platelet adhesion, aggregation, participation in inflammatory responses, and for triggering degranulation and enhancing thrombin generation. Modifications of platelet membrane receptors can be involved in the alteration of platelet function. However, platelet functions and their clinical impacts in CMML have never been addressed. The main aim of this study is to understand how different platelet surface proteins (receptor) expression, platelet activation, aggregation, and clot formation play role in CMML patients with thrombocytopenia. Results from this study will lead to a better understanding of platelet populations and function in CMML patients.
Research opportunities: If you are a master's student and interested to do research within our group as a degree project, please contact with me.