About me
Per Uhlén is Professor of Cell Signaling in the Unit of Molecular Neurobiology at the Department of Medical Biochemistry and Biophysics (MBB), Karolinska Institutet
Research description
Research Insterests
Per Uhlén’s research group is studying cancer and developmental related processes in various types of cells. The biological platforms that we are applying are tumor samples, tissue slices, primary cell cultures, cell lines and stem cells. To study the processes of cell signaling, cell division, cell differentiation, and neural migration/networking we are applying state-of-the-art imaging techniques, such as 3D light-sheet microscopy and tissue clearing, advanced analysis tools, such as Fourier transform and deep learning, and modern genetic tools, such as transgenic animals. Our focus is to shed light on the intracellular cell signaling mechanisms and biological processes involved in disease and development. We are particularly interested in calcium signaling and how cells are modulating and decoding the frequency/amplitude of intracellular calcium oscillations.
Selected Key Publications ( ‡ corresponding author )
Zhang S, Miyakawa A, Wickström M, Elfman L, Louhivuori L, Varas-Godoy M, Kemppainen K, Kanatani S, Kaczynska D, Dehnisch Ellström I, Dyberg C, Kronqvist P, Repo H, Mikoshiba K, Sahlgren C, Johnsen JI, and Uhlén P‡ “GIT1 Protects against Breast Cancer Growth through Negative Regulation of Notch”
Nature Communications Mar 22;13(1):1537 (2022)
Smedler E, Louhivuori L, Romanov RA, Masini D, Dehnisch Ellström I, Wang C, Caramia M, West Z, Zhang S, Rebellato R, Malmersjö S, Brusini I, Kanatani S, Fisone G, Harkany T, Uhlén P‡ “Disrupted Cacna1c Gene Expression Perturbs Spontaneous Ca2+ Activity Causing Abnormal Brain Development and Increased Anxiety”
Proc Natl Acad Sci USA Feb 15;119(7):e2108768119 (2022)
Tanaka N, Kanatani S, Kaczynska D, Fukumoto K, Louhivuori L, Mizutani T, Kopper O, Kronqvist P, Robertson S, Lindh C, Kis L, Pronk R, Niwa N, Matsumoto K, Oya M, Miyakawa A, Falk A, Hartman J, Sahlgren C, Clevers H, Uhlén P‡ “Three-dimensional single-cell imaging for the analysis of RNA and protein expression in intact tumour biopsies”
Nature Biomedical Engineering Sep;4(9):875-888 (2020)
Uhlén P‡, Tanaka N “Improved Pathological Examination of Tumors with 3D Light-Sheet Microscopy”
Trends in Cancer May;4(5):337-341 (2018)
Tanaka N, Kanatani S, Tomer R, Sahlgren C, Kronqvist P, Kaczynska D, Louhivuori L, Kis L, Lindh C, Mitura P, Stepulak A, Corvigno S, Hartman J, Micke P, Mezheyeuski A, Strell C, Carlsson JW, Moro CF, Dahlstrand H, Östman A, Matsumoto K, Wiklund P, Oya M, Miyakawa A, Deisseroth K, Uhlén P‡ “Whole-tissue biopsy phenotyping of three-dimensional tumours reveals patterns of cancer heterogeneity”
Nature Biomedical Engineering Oct;1(10):796-806 (2017)
Smedler E, Malmersjö S, Uhlén P‡ “Network Analysis of Time-Lapse Microscopy Recordings”
Front Neural Circuits. Sep 17;8:111 (2014)
Malmersjö S, Rebellato P, Smedler E, Planert H, Kanatani S, Liste I, Nanou E, Sunner H, Abdelhady S, Zhang S, Andäng M, El Manira A, Silberberg G, Arenas E, Uhlén P‡ “Neural Progenitors Organize in Small-World Networks to Promote Cell Proliferation”
Proc Natl Acad Sci USA Apr 16;110(16):E1524-E1532 (2013)
Desfrere L, Karlsson M, Hiyoshi H, Malmersjö S, Nanou E, Estrada M, Miyakawa A, Lagercrantz H, El Manira A, Lal M, Uhlén P‡ “Na,K-ATPase Signal Transduction Triggers CREB Activation and Dendritic Growth”
Proc Natl Acad Sci USA Feb 17;106(7):2212-7 (2009)
Uhlén P‡, Burch P, Ivins Zito C, Estrada M, Ehrlich BE, Bennett AM “Gain-of-Function/Noonan Syndrome SHP-2/Ptpn11 Mutants Enhance Calcium Oscillations and Impair NFAT Signaling”
Proc Natl Acad Sci USA Feb 14;103(7):2160-5 (2006)
Uhlén P‡ “Spectral Analysis of Calcium Oscillations”
Science STKE Nov 09;(258):pl15 (2004)
Uhlén P, Laestadius Å, Jahnukainen T, Söderblom T, Bäckhed F, Celsi G, Brismar H, Normark S, Aperia A, Richter-Dahlfors A ”a-haemolysin of Uropathogenic E.coli Induces Ca2+ Oscillations in Renal Epithelial Cells”
Nature 405, 6787, 694-697 (2000)