Inverted Zeiss microscope with Eppendorf microinjection system
The LCI core facility is selling a Zeiss inverted manual microscope Axiovert S100 to which an Eppendorf microinjection system with micromanipulator Transjector 5246 is attached.
The manipulator x and y motors still work as they should. The pump has not been tested.
The microscope has a camera (AxioCamMRc) and light sources for transmitted light and for fluorescence (X-Cite 120). The light guide is old and likely needs to be changed.
The microscope has several Phase contrast objectives and condenser lenses.
The manual for the microinjector is included.
Full specs:
Objectives:
- 10x, NA 0.25, A-Plan, Ph1, 44 10 31
- 20x, NA 0.3, Achrostigmat, Ph1, 44 01 47
- 32x, NA 0.4, Achrostigmat, Ph1, ∞/0.5-1.5
- 40x, NA 0.6, Korr, LD Achroplan, Ph2, ∞/0-2
Filter cubes:
- Blue channel: Ex 360/40; Em ?
- Green channel: Ex 470/20; Em 505-530
- Red channel: Ex 545/30; Em 620/60
- FarRed channel: Ex 620/60; Em 690/50
- CFP channel: Ex 436/20; Em 480/40
- YFP channel: Ex 500/20; Em 535/50
Camera: AxioCam MRc, B/W, 1388x1040 pixels, pixel size 6.45 um