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Laboratory safety in Biomedicum

Laboratory safety includes rules, regulations and handling instructions in the areas of biosafety, chemical safety, radiation safety and fire safety. It also includes waste management, risk assessments and environment and sustainability.

Biosafety

Biosafety means the protection of people and environment from exposure to contagious biological material generated in the laboratory.

Issues regarding biosafety safety can be reported to the department's contact person for laboratory biosafety or to the Biosafety coordinator in Biomedicum, Carina Bengtsson, or to the Biosafety coordinator at Karolinska Institutet, Jenny Karlsson.

Useful links:

► Biosafety at Karolinska Institutet
► Commom facilities and labs in Biomedicum
Permits and risk assessment
► Registration, permits and risk assessment for GMM

Biomedicum's Biosafety officers

Björn Andersson

Professor
070-355 39 87
C5 Department of Cell and Molecular Biology

Albert Blanchart Aguado

Laboratory coordinator
C2 Department of Medical Biochemistry and Biophysics

Stefano Gastaldello

Senior researcher
08-524 867 81
C3 Department of Physiology and Pharmacology

Antonio Gigliotti Rothfuchs

Senior researcher
08-524 852 52
C1 Department of Microbiology, Tumor and Cell Biology

Håkan Karlsson

Senior researcher
08-524 878 32
C4 Department of Neuroscience

Chemical safety

Karolinska Institutet conducts many chemically and environmentally hazardous activities. Chemical handling is surrounded by many laws, rules and regulations - both from Sweden and the EU - covering work environment as well as external environment issues. The purpose of these regulations is to create a safe work environment, to protect the external environment and to ensure a sustainable development.

Room for handling CMR-classified chemicals in Biomedicum.

Useful links

► Chemical safety at Karolinska Institutet
KI's Emergency procedure for larger spills of hazardous chemicals.

There are a number of hazardous chemicals requiring special handling - Substances with special rules, prohibition or permit requirements.

Issues regarding chemical safety can be reported to the Chemical Safety Coordinators in Biomedicum, Sabina Eriksson, or to the Chemical Safety Coordinator at KI

Barcodes and purchases of chemicals

A barcode system for identification and registration of chemicals will increase the efficiency of handling chemicals and improve the chemical safety.

All chemicals in Biomedicum will have a barcode with information about name, CAS number, quantity and storage location. This provides a continuously updated list in KLARA of all chemicals, for instance information of storage location, which is extremely important in case of fire or an accident.

All chemicals must be labelled with a barcode before they are relocated. All storage spaces in Biomedicum will be labelled with a barcode, which means that relocated chemicals can quickly be registered at a new storage location.

If chemicals are to be stored in other places, please contact FM Helpdesk for help with generating a new barcode.

When purchasing new chemicals, the user places an order in KLARA's purchase module, which also indicates the storage location. A purchaser at FM, will regularly compile all orders and send them to the respective supplier. Upon delivery, the chemicals labelled with a barcode prior transported to the user.

The purchase of chemicals via KLARA will soon be available. In the meantime, you may order your chemicals in the ordinary way. Please note that the chemicals you order will upon arrival need barcodes. You may print them yourself or contact FM's help desk for assistance.

KLARA chemical register

According to the law (AFS 2014:43 and SFS 1998:901) all organizations and companies must keep a register of chemical products and biotechnical organisms that may pose a risk to health and/or environment.

At Karolinska Institutet, this registry is conducted in KLARA and kept up to date by an annual inventory at the beginning of each year.

Log in KLARA

    Fire safety routines

    For emergency routines, follow the procedures in case of emergency in Biomedicum.

    You will find more information about Fire Safety at Karolinska Institutet and Karolinska Institutet's fire safety rules on the KI Staff portal.

    For more information on technical fire safety installations such as alarms and sprinklers, see property owner Akademiska Hus User guide Biomedicum (pages 12-16)

    Fire safety organisation

    The KI’s fire safety organisation is designed so that the departments themselves can take responsibility for the working environment and deal with fire safety issues that arise within their operations.

    The Facility Management support the departments and work to inform, guide and organize fire safety activities at Biomedicum. More information on the different roles within the Fire safety organisation.

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    Biomedicum fire safety organisation

    By working systematically with fire safety, KI shall ensure that the risk of a fire breaking out and the consequences of such an event are minimized as far as possible. Preventive action, training, self-monitoring, routines and guidelines play key roles here.

    As an individual, you have an important part to play in minimizing the risk of a fire, and everyone at KI has a joint responsibility to protect our working environment.

    It is the responsibility of all staff to follow the KI fire safety rules, to daily make sure that evacuation routes are kept clear and problems with emergency showers, fire blankets or fire extinguishers are reported to the fire safety supervisor.

    fire warden
    Fire warden at evacuation station.

    Evacuation Leaders/Fire Wardens

    We do not have appointed fire wardens at KI. The organization is based on the strategy that anyone can act as a fire warden. The reason why KI has chosen this type of evacuation organisation is that KI employees are highly mobile and a high level of staff turnover.

    In the event of an evacuation situation, the first person to reach the evacuation station puts on the vest and follows the instructions on the Fire Warden instruction card.

    The Fire Warden is responsible for going through the premises and instructing colleagues to evacuate to the assembly point. However, it is important to emphasize that everyone has a personal responsibility to evacuate, and for following the Fire Warden’s instructions.

    It is the Fire Warden who contacts the Fire Brigade or a technician from Akademiska Hus when they arrive on site and for announcing – when confirmed by the Fire Brigade or the technician – that it is safe for employees to re-enter the building.

    Read more about the Biomedicum evacuation strategy at procedures in case of emergency in Biomedicum.

    Fire safety training

    It is important that you feel safe on campus and at your workplace. Knowing what to do in the event of a fire alarm and how to deal with a fire at your workplace is an important part of this, as is knowing how to prevent fires within the context of your work.

    KI offers all employees fire safety training, including both theoretical and practical content.

    Book the course Basic Fire Education.

    More information about Karolinska Institutet's Fire Safety Training can be found on the KI Staff portal.

    Workplace introductions

    In addition to KI fire safety training, new employees should also be given an introduction in workplace fire safety.

    The group leaders are responsible for ensuring that new employees receive introductions, but it is normally the Fire Safety Controller at the quarter who carries out the actual induction.

    A checklist has been drawn up, detailing the points that employees need to know about - Checklist for workplace fire safety introduction for new employees.

    Fire safety supervisor at Biomedicum

    Sabina Eriksson

    Coordinator
    C3 Department of Physiology and Pharmacology

    Flammable goods controller:

    1-2 persons from each quarter or group (contact information to be updated shortly)

    FM is responsible for all common areas.

    Fire safety controller:

    1-2 persons from each quarter or group (contact information to be updated shortly)

    FM is responsible for all common areas.

    • DAILY: Evacuation routes should be kept clear and problems with emergency showers, fire blankets or fire extinguishers should be reported to the fire safety supervisor.
      Responsible: All staff
    • QUARTERLY: Inspection of emergency routes, emergency signs, emergency showers and fire blankets. Ensuring that fire extinguishers are checked. Any problems are reported to the fire safety supervisor
      Responsible: The fire safety controllers
    • ANNUALLY: External party revisions of fire extinguishers.
      Responsible: Central administration at Karolinska Institutet and Akademiska Hus.

    Handling of gas

    For more information on Biomedicum gas storage rooms, see Gas storage room.

    Carbon dioxide (CO2), Nitrogen (N2) and Carbogen (CO2 + O2) are gases that are centrally provided in Biomedicum.

    Other/special gases are purchased via FM. Contact FM Helpdesk for help and guidance when you want to purchase gas.

    Different types of gas and their risks:

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      Hazard pictograms (flammable)

      Category: Flammable

      Examples: Methane, Ethane, Acetylene, Hydrogen.

      Risk: When mixed with air or oxygen, flammable gases can combust and may explode if they are ignited.  The likelihood that a flammable gas will ignite is affected by its flammability range. The primary hazard from a flammable gas is the risk of fire and explosion, in addition flammable gases have the hazard of asphyxia and some have narcotic effects.

       

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      Inert gas

      Category: Non-flammable, Non-toxic (e.g. inert gases)

      Examples: Nitrogen, Helium, Argon, Carbon dioxide.

      Risk: Properties: Non-flammable, non-toxic gases include gases which are generally referred to as inert, as they are not reactive. Inert gases are non-oxidising, non-flammable and non-toxic but may dilute or displace the oxygen normally present in the atmosphere. The primary hazard from inert gases is therefore asphyxia, or for CO2 - carbon dioxide intoxication.

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      Hazard pictograms (toxic)

      Category: Toxic

      Examples: Hydrogen sulphide, Anhydrous ammonia, Carbon monoxide.

      Risk: Toxic gases impact adversely with people to varying degrees, from a mild irritant, to a severe reaction including death, dependent on the concentration and the susceptibility of the individual.

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      Oxidising agent

      Category: Oxidising

      Examples: Oxygen, Carbogen, Nitrous oxide.

      Risk: Oxidising gases will support the combustion process.  Many substances which would otherwise not combust, are able to combust and burn fiercely in an atmosphere enriched with oxidising gases. The primary hazard from oxidising gases is the increased risk of combustion, coupled with an increased intensity of combustion.

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      Hazard pictogram (corrosive)

      Category: Corrosive

      Examples: Sulphur dioxide, Chlorine, Hydrogen chloride.

      Risk: Corrosive gases chemically attack and damage skin, eyes and mucous membranes on contact.

      Gas storage requirements in Biomedicum

      Flammable gas

      Flammable should be stored in the quarter gas storage rooms. A central pipeline distribution system will provide your equipment/lab space with the gas.  

      Flammable gas can also be stored in a ventilated EI 90 classified cabinet/storage, from which a pipeline distribution system provides the equipment/lab space with the gas.  

      Exception: You are allowed to use the smaller camping size gas containers on the benches. Remove to a proper storage when not in use. You may keep a few of the small camping size gas containers in the yellow EL-classed flammable cabinets.

      Oxidizing gas

      Oxidizing gases should firsthand be stored in the gas storage room and have a central pipeline distribution system that provide your equipment/lab space with the gas.

      Note: oxidizing gases should not be stored together with flammable gases.

      Contact FM Helpdesk to discuss potential alternatives.

      Inert gas

      Inert gases can be stored in the quarters, but the size limit of the cisterns in the open laboratories is 20L. When not used, the cisterna should be stored in the gas storage room. Gas cisterns should always be stored standing up and chained to a wall or bench.

      Note: inert gases may dilute or displace the oxygen normally present in the atmosphere and might still require some kind of safety installations such as oxygen alarm or similar.

      Contact FM Helpdesk to discuss what installations that might be relevant to you.

      Gas Handling routines

      • Only qualified personnel are allowed to handle gas cisterns and gas systems. Never let students or personnel that are not trained handle gas!
      • Gas should be purchased via FM, contact FM Helpdesk for more information.
      • Handling of gas or installations of gas to laboratory equipment requires a risk assessment and handling instructions. The risk assessment should answer the questions: which gas(es)? what are the hazards? what are the risks? what is the acceptable risk level? what are my control measures? what do I do in case of emergency?
      • All chemical gas cisterns must be stored standing up and chained to a wall, without the need to use any tools. When in transport the cisterns must be transported with a proper gas-trolley with chain.
      • Do not remove the supplier labels on the cisterns.
      • All gas cisterns should be labelled with name and phone number to a contact person.
      • Never expose gas cisterns to heat.
      • Never use flammables to test for leakages!
      • In case of potential leakages, contact AGA on phone +4687069500 and Akademiska Hus jour +468 685 76 87 and FM Helpdesk.
      • Never store flammable materials such as cardboard in a gas storage location.
      • Empty and full gas cisterns should be stored separately.
      • Flammable gases should be kept from the other types of gases in the gas storage room. Never store flammable gases with oxidizing gases such as oxygen.

      If you have any questions/concerns about handling gas, contact FM Helpdesk or AGA on phone +4687069500.

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      Label for contaminated lab coats to be autoclaved.

       

      Good Laboratory Practices

      • Good Laboratory Practices (GLPs) are to be followed in the laboratories at all times;
      • Do not eat, drink, apply cosmetics, use tobacco products, or handle food in the lab.
      • Wear personal protective equipment such as lab coats and gloves in the lab, but never in the office areas or common areas.
      • Observe cleanliness and maintain good order in your work environment.
      • Avoid spills, splashes and formation of aerosols when handling liquids or powders.
      • Handle needles and sharps safely. Dispose of these in appropriate sharps containers.
      • Manage waste safely and responsibly. Use correct bins and labeling.
      • It is OK to make mistakes! If you are unsure on how to safely handle a situation, materials, or of the risk associated with any procedure, always seek guidance!


      Personal Protective Equipment (PPE)

      Appropriate personal protective equipment (PPE) is to be used at all times in the laboratory.
      PPE will protect you against;

      • Exposure to chemicals or biological materials
      • Eye contact with hazardous or corrosive liquids and solids
      • Inhalation of toxic or irritating aerosols/fumes
      • Radiation (ionizing or UV)

      PPE is also used to protect your experiments from contamination or other interference.

       

      Lab coats

      Lab coats should be worn in the laboratories but removed when leaving the lab and when entering common and office areas.

      • Clean lab coats can be picked up from the room for clean lab glass ware on each floor. If you need clean but autoclaved lab coats, send a ticket to FM Helpdesk to order autoclaved lab coats.
      • Dirty lab coats should be placed in the waste room and will be transported to floor 2 by FM-staff.
      • If you have a contaminated lab coat that needs to be autoclaved (decontaminated) prior to being washed, place it in an autoclave bag and label with the autoclave label available in the quarter waste rooms. Heavily contaminated lab coats should be disposed of in the yellow laboratory waste bins.

      Safety glasses

      Protective glasses should always be worn when working with the following materials outside of a shielded environment (i.e. fume hoods or ventilated cabinets);

      • Chemicals. Regardless of concentration, chemicals should always be considered toxic.
      • UV light or hot fumes (ånga).
      • Sharps or cutting objects.
      • Bacteria, cell cultures, viruses, GMM, blood/tissues.
      • Working with cold substances at low enough temperatures to cause damage, such as liquid nitrogen and dry ice. Padded gloves should be used.

       

      Please note;

      • The use of contact lenses might increase the risk of eye injury if exposure occurs.
      • Use safety glasses with full cover when working with toxic/corrosive chemicals, if standard laboratory glasses cannot provide full protection.

       

      Respiratory protection

      Respiratory protection masks should always be worn when working outside of a shielded environment (such as ventilated workspace) with hazardous materials that might create hazardous aerosols or fumes. Respiratory mask should for example be used when handling the following materials outside of a shielded environment: chemicals that can have an impact on your respiratory tract, CMR and toxic chemicals, viruses and blood-samples.

       

      Gloves

      Protective gloves should always be used when working with:

      • Chemicals. Regardless of concentration, chemicals should always be considered toxic
      • UV light or hot fumes/steam
      • Sharp or cutting objects
      • Bacteria, cell cultures, viruses, GMM, blood/tissues.
      • Cold substances at low enough temperatures to cause damage, such as liquid nitrogen and dry ice. Extra padded gloves should be used.

       

      Gloves should be removed when;

      • Leaving the lab
      • Using computers or other equipment such as keyboards or dials.
      • Rule of thumb: No touching of any door handles with gloves!

        Lab glass

        There is a central lab washing facility in Biomedicum.

        • Place used lab glass ware in the waste room in your quarter.
        • FM will collect all used lab glass ware from the quarters and transport it to the central dish washing unit on floor 2 on a daily basis.
        • Cleaned and, if needed, sterilized lab glass ware will be placed in the storage room (one per floor).
        • If you have group-specific lab glass ware, place it in a ‘container’ marked with your name and quarter. This will ensure that you will receive the same glass ware.

        Follow special instructions if the lab glass ware is contaminated and needs to be autoclaved prior to dish washing. In the lab where the lab glass is used, place the contaminated lab glass in an autoclave bag and seal with autoclave tape. Only then can the bag be placed in the container marked ‘To be autoclaved’ in the waste room.

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          Label for items to be autoclaved.

          Items to be autoclaved

          Pipette tips, tubes, tools, solutions, etc. to be autoclaved

          • Place item in an autoclave bag
          • label with name, quarter and the date when you need it.
          • Put the bag in designated place in the room for clean lab glass ware (one per floor).

          If it is done prior to noon, it will be delivered back the same day.

          Laboratory Safety Test

          In order to further improve the safety in our laboratory environment, everyone working in Biomedicum will have to pass a Laboratory safety test. The introduction test has been developed to ensure that everyone working in the premises or the laboratories at Karolinska Institutet has the same basic understanding of safety.

          More information on the Laboratory Safety Test can be found on the Staff portal.

          Liquid nitrogen and dry ice

          Users register an errand in the Help desk, where the needed volume and delivery date are stated. FM will transport the liquid nitrogen via the prioritized elevator to the user.

          Dry-ice (blocks and/or pellets) are kept available on level 2. Small quantities can be picked up by the users when needed. Larger quantities can be ordered separately and delivered to the desired internal address.

          Low temperature freezer storage facility in Biomedicum.

          Narcotics and pharmaceuticals

          As a state-owned scientific institution, Karolinska Institutet does not require a permit for possession of narcotic drugs necessary for scientific research, examination or teaching (Law on Control of Narcotic Drugs, 1992:860, 7§).

          Purchasing, handling and storing narcotics and pharmaceuticals is however subject to certain rules and regulations – see Karolinska Institutet Narcotic drugs and narcotic drug precursors.

          More information available on Swedish Medical Agency (Läkemedelsverket). See also their list of drugs classified as narcotics.

          Read also Routine for ordering, handling and storage of narcotics in Biomedicum.

          How to order narcotics and pharmaceuticals in Biomedicum

          Departments using narcotic drugs or narcotic precursors often have a pharmaceutical manager who's responsibility is to make sure rules and regulations are followed. They also help with the ordering of substances requiring a prescription, including narcotic drugs.

          In Biomedicum you have the possibility to order narcotics and pharmaceuticals either via your department’s pharmaceutical manager, or FM's.

          To order narcotics and/or pharmaceuticals via the FM Pharmaceutical Managers

          Contact FM Helpdesk, and clearly state the following in your order:

          • Name of the substance
          • Item number
          • Concentration
          • Volume
          • Your name
          • Address
          • ZZ-code
          • Project number
          • Number of the ethical permit

          Biomedicum FM Pharmaceutical Managers

          Carina Bengtsson

          Coordinator
          08-524 862 89
          UF Central Administration

          Sabina Eriksson

          Coordinator
          C3 Department of Physiology and Pharmacology
          etikett förorening labutrustrning
          Decontamination labels to be used when handling excess laboratory equipment.

          Radiation protection

          All work-related activities with radioactive substances or technical devices capable of generating ionising radiation must be carried out in accordance with the Swedish Radiation Protection Act (1988:220), the Radiation Protection Ordinance (1988:293), Regulations from the Swedish Radiation Protection Authority and local radiation protection rules.

          Radioactive facilities in Biomedicum.

          Read Karolinska Institutet's rules and guidelines on Radiation protection.

          Strålsäkerhetsmyndigheten / Swedish Radiation Safety Authority

          Water

          There are special taps for clean water that are placed in the room for apparatus in each quarter. This water has very high quality and is the same or even higher quality than MilliQ-water. Clean water is the same thing as ASTM type II or HPW (High purified water).

          In the central lab dish unit located on floor 2, there is a MilliQ-machine (Milli-Q IQ 7000 Ultrapure Lab Water System) for the production of MilliQ-water. This water is tapped in designated glass bottles only used for this purpose and then autoclaved. The bottles are delivered and stored in the room for clean lab glass ware at each floor.

          Please note that it is not allowed to install old MilliQ-machines. This will highly increase the risk for contamination of the clean water.

          Waste Management

          Read more on Waste management specific to Biomedicum.

          Consult Karolinska Institutet's rules and guidelines for Laboratory waste.

          How to handle excess laboratory equipment

          Checklist with guidelines to follow:

          • Clean/decontaminate the equipment accordingly (depending on what it has been used for).
          • Fill out, sign and place the decontamination label/sticker on the equipment.
          • Contact the financial officers at your department to find out if there is a value registered in the financial system. The dialogue with your accountant/financial officer should continue until the process is finished.
          • The equipment should first and foremost be used within KI. Hence, publish information about your excess equipment on Buy and sell on the Staff portal. Fill in and send the form I have lab equipment to sell/give away.
          • If no one within KI is interested and the ad has been published for 2-3 weeks, it is ok to sell it outside of KI. The price should then be set at market value. Please contact the financial officers at your department for advice.
          • If equipment is sold to an employee within KI, the price must be set at market value including VAT.
          • Equipment that is useless and without value can be discarded. Contact FM Helpdesk for assistance. Please note that equipment without correct labelling will not be handled by FM
          • Please observe that decisions about giving away equipment cannot be taken on group level, since permission from the government is needed.
          waste basket
          Waste baskets for transportation of material between quarters.

          Useful tips

          Waste baskets

          To ensure a safe transport of different material within and between quarters, there are baskets available in the waste room in your quarter.

          Remember to keep the basket clean and wipe it of if it´s gets contaminated. Put it back in the waste room when you are done.