Chemical safety

Chemical risks are the risks that can arise from the handling of chemical products according to the working environment, the external environment and safety. All employees at KI who handle chemical products at their workplace must be familiar with the laws, rules and procedures that apply in the field and are responsible for following them.

Definitions

(according to section 4 in AFS 2011:19)

Chemical product
Chemical substance or mixture of chemical substances which have been manufactured or extracted and whose function is primarily determined by its chemical composition or constitutes waste.

Chemical hazard
Chemical product, chemical substance or several chemical substances together which can cause ill-health or accidents through.

• properties which make it hazardous to health
• its properties when it depends on the way in which the substances are used or occur
• its temperature
• reducing the level of oxygen in the air
• increasing the risk of fire, explosion or other hazardous chemical reaction.

Mold spores and chemical substances released from microorganisms that can cause allergies and toxic effects are also included in the definition of a chemical hazard.

Handling 
Manufacturing, processing, treatment, packaging, storage, transport, use, disposal, destruction, conversion and comparable procedures.

KLARA product database

For questions regarding KLARA, please contact your departmental chemicals representative first, and if necessary, KI's central system administrator, Erik Stenholm.

According to the law and regulations all organizations and companies must keep a register of chemical products that may pose a risk to health and/or environment. 

At KI, we use KLARA to maintain a register of all chemical products handled, including those that are not hazardous, as well as substances that are synthesized or manufactured in-house.

The register must be updated continuously and at the beginning of each calendar year KI conducts a chemical inventory in KLARA.

There is also a risk assessment tool available.

In KLARA it is possible to:

• search for information of chemical products in the product database
• keep chemical register and perform inventories (possible with bar code)
• extract lists and reports
• conduct risk assessments.

Profiles in KLARA

All employees/students who handle chemicals in their activities must have an account in KLARA. Accounts are personal, and login information must never be shared with others.

To create an account with the associated profile, log in to KLARA using your KI-ID. After that, contact your departmental chemicals representative, who is responsible for managing profiles in KLARA and assist in selecting the appropriate profiles for each employee/student.

The following profiles are available:

• Chemical inventory taker: One or more per group/unit who are responsible for updating the chemical register and conducting the annual chemical inventory. This profile is delegated by the nearest manager.
• Risk Assessor: Anyone who needs to perform risk assessments, including the responsible supervisor for review and signature.
• Risk Assessor – Read: Anyone who needs to read approved risk assessments.
• Read Access (applies to the chemical register): Anyone who needs to locate chemicals and access safety information.

To get a profile as a chemical inventory taker you need to complete a KLARA inventory course at KI, and you need a signed delegation from your nearest manager. More information about the delegation and a role description can be found here.

Information on current KLARA courses is published in KLARA. Select “Course booking” (left column of KLARA’s start page).

KLARA - Chemical inventory

At the beginning of each year, all research groups at KI that store chemicals must conduct a mandatory chemical inventory in KLARA. The inventory is typically open for 8 to 12 weeks, and its purpose is to update the chemical register. 

The inventory forms the basis for the reports that KI is required to submit to various regulatory authorities.

During the inventory, the register shall be updated with regards to:

• Products (name, CAS, KLARA-ID, concentration, content etc.)
• Quantities (amount per container / package)
• Maximum stored amount - all flammable products incl. liquids and gases must be registered with ”max storage”, more information here.
• Suppliers (from whom the product is purchased)
• Safety data sheet (SDS, from the supplier from which the product is purchased, must be in Swedish and according to CLP / REACH)
• Classification (according to the supplier's SDS and according to CLP / REACH)
• Premises (right campus, building, house, floor and room number)

Kits, cell media, buffers and all gas containers must be registered.

Narcotic drugs and other pharmaceuticals should not be registered in KLARA. 

Antibodies and proteins do not need to be registered unless they are mixed with chemicals (e.g. sodium azid often used as a preservative).

It is recommended to use KLARA barcodes to increase security and save time and resources. Read more about the barcode function below.

Contact the department's chemical representative for help with account and authorization in KLARA as well as, if needed, support around KLARA and inventory.

Send new or updated SDS to newproducts.klara@ecoonline.com .

KLARA - Barcodes

To improve the work with safe handling of hazardous chemicals and to simplify the annual chemical inventory procedure, KI has implemented a barcode application in the KLARA product database.

Presentation of the different features offered in the new barcode application:

1) Basic function used for the annual chemical inventory
KLARA generates a unique barcode for each product, which is printed and placed on the container. A barcode reader is used during the inventory to register chemical products in order to update the chemical register.

It is recommended to also generate a barcode for the storage (e.g. room / cabinet / shelf).

2) Advanced function used for safe storage
Products are tagged with a chemical property, for example acid, base, flammable, poison etc. Storages are tagged in the same manner and the barcode reader is used for "logging in" and "logging out" chemicals. The user will receive a warning from the system if the chemical is logged in to a storage with wrong tagging, for example if an acid is placed among the bases.

3) Advanced function used to keep track of the chemicals
If the lab has many chemicals, it may be useful to use the barcode application to keep track of the chemicals. Each storage and each person get a unique barcode and the system is used for "logging in" and "logging out" the products, e.g. a product always has a position; it is either placed in a storage or with a person.

4) Logging in to KLARA barcode app using personal barcode
To simplify the features described in issue 3, it is possible to use a personal barcode for logging in to the KLARA barcode app. This is particularly useful if the lab has a shared computer designed for the purpose and placed in the chemical stock room.

Get started with barcodes: For using KLARA barcodes you need to have an inventory taker profile in KLARA. In addition, a special profile is required for KLARA barcodes. Contact the department’s chemicals representative for assistance with permissions in KLARA as well as information on installing the barcode app. Barcode app can be downloaded from the Software Center, “KLARA - BarcodePcApp”.

You also need an configuration file. You get access to the file by contacting erik.stenholm@ki.se.

Printers and scanners (funded by the department/research group):

• Printer: Zebra ZD421. (Previous model GK42-102520-000 is no longer available for purchase.)
• Scanner/barcode scanner: both wireless and cordless. Must be able to read code128.
• Labels: Zebra CryoCool 8000T Polypropylen 30x15mm; Zebras part no. 3009674. (The same labels are used for previous printer model.))
• Color ribbon: Zebra 5095 Resin 64mmx74m; Zebras part no. 05095GS06407. (The same color ribbon is used for previous printer model.)

The size of the label is adapted to the needs, these labels above are only recommended dimensions for "regular" use.

Printers and scanners can be ordered via KI's framework agreement. Most importantly, the printer is compatible with the KLARA barcode app (compatibility questions, contact KLARA Support), so it is recommended to use the specified printer, but there may be other options that work as well.

Scanner selection is more flexible and dependent on the need. If the system is to be used for logging in and out of chemical products, it may be wise to invest in a wireless scanner.

If you have problems with the installation of KLARA BarcodePCApp from the Software Center as well as drivers for the current printer to be used, create a case under IT support at KI. Use “KLARA barcode” as case title.
Note! The person from the Helpdesk will not provide support in the laboratories. Test the equipment outside the laboratory!

KLARA - Structure and substructure search

Instead of searching for chemical products in KLARA using product names, IUPAC nomenclature, trivial names, CAS or KLARA ID, it is also possible to search for structure, "exact structure" or "substructure".

In order to add the structure of a molecule in KLARA the person’s profile in KLARA has to include "write user molecular structure", i.e. the right to insert new molecular structures into KLARA. To do this, one must be an organic chemist or equivalent, e.g. that you have sufficient knowledge of molecular structures.

You can manually draw new structures in the drawing tool, but you can also import so-called MOL files.

Safety Data Sheet (SDS)

The information in KLARA is mostly based on information from supplier’s SDS:s, e.g. product names, synonyms, article numbers, classification, ingredients etc. As a user of chemical products in your work, you are required to have access to the chemical products SDS when handling hazardous chemicals. 

There are many in the workplace who may need to use the SDS:s, primarily those who handle the products, but many more should be informed about the chemical risks. Persons working in connection with the source of risk, e.g. cleaning staff, technicians and administrative staff, even environmental coordinators, purchasers and transport managers may also need access to SDS:s.

Here are some common tasks that SDS:s are used for:

• Conduct risk assessments of the environmental and health risks associated with the chemical product in question, as a basis for designing safe workplaces and working methods
• Provide a basis for emergency procedures and emergency support
• Be a support when purchasing chemical products
• Provide a basis for chemical targets / substitution / phasing out of hazardous chemicals
• Provide a basis for how products should be treated as waste

The supplier must deliver SDS to the customer at least with the first delivery. The sheet should be free of charge, written in Swedish and dated. It should not be older than two to three years. It is common for SDS:s to be sent in electronic form. However, the recipient must first approve this.

It can also be a valuable service that the sheets are also available on the company's website. There sometimes are deficiency in the supplier’s safety data sheets. Therefore, do not be afraid to contact the supplier to make comments or request supplements. A safety data sheet should contain 16 different main headings.

Special requirements for allergenic chemical products

Some chemical products can cause allergic reactions. It may be allergic skin reaction, asthma symptoms or breathing difficulties. For an allergic reaction to occur you need to be exposed to an allergenic substance. It is important to avoid exposure to allergenic chemical products because if you get an allergy caused by exposure to an allergenic substance you can get symptoms from a very small amount of the substance.

Special requirements

If a risk assessment shows that there is a risk for exposure to any of the chemicals beneath, there are special requirements. 

• chemical products labelled with H317 (may cause allergic skin reaction) or H334 (may cause asthma symptoms or breathing difficulties)
• chemical products which contain ethyl- or methyl-2-cyanoacrylate
• work that entails thermal degradation of materials that release iso-cyanates or processes that release formaldehyde

It must be clearly stated in the documentation for the risk assessment:

• the areas and spaces where the hazardous chemical products shall be handled
• the protective measures necessary to ensure that the exposure is as low as possible
• which protective equipment to use and in which situations it is required
• how the function of equipment and ventilation is to be checked and maintained to prevent allergenic substances from causing ill-health

If there is a risk for other employees to be exposed in the area where the substances are handled, signs must be posted. Also, those who are leading or are employed in the work with the substances mentioned above should be informed about how to carry out these tasks safely and about the risks involved with the handling.

Training

Training is required in order to lead or be actively employed in work with hazardous chemical products that contain (AFS 2011:19 §37e):

1. diisocyanates
2. epoxy plastic components
3. organic acid anhydrides
4. formaldehyde resins
5. methacrylates that shall be labelled with H317 or H334
6. acrylates that shall be labelled with H317 or H334
7. any work that entails the thermal degradation of materials that release isocyanates or processes that release formaldehyde.

Training is also required for work with chemical products containing ethyl-2-cyanoacrylate or methyl-2-cyanoacrylate, if the work is carried out for more than a total of 30 minutes per week.

The training shall at least contain information about the risks entailed by the work, and the protective measures that may need to be taken. A training certificate should be obtained, and the certificate may not exceed five years. Training certificates must be available and by request shown otherwise it can lead to a penalty fee of 10,000 SEK per employee.

For booking of the training please contact the Chemical safety coordinator, see contact below. 

Medical check-up 

For some of the work with allergenic chemical products it is required to do a medical check-up with or without aptitude report, see section 37 f and g in the provision Chemical hazards in the working environment 2011:19. Also see the provision about Medical check-up (only in Swedish: Medicinska kontroller), AFS 2019:3. In case a aptitude report is required, a penalty fee is applied when the aptidude report is missing.

More information about medical check-up under page Statutory medical check-ups.

CMR-classified chemicals

AFS 2011:19 § 38-44 states the rules that apply for handling CMR-classified products (carcinogenic, mutagenic or toxic to reproduction).

CMR classified chemical products are those marked with hazard statements and/or risk phrases:

H350: May cause cancer
H340: May cause genetic defects
H360: May damage fertility or the unborn child

A CMR classified chemical product is only allowed to be handled if there is a documented investigating showing that it is not technically possible to replace the product with a less hazardous product. A form for this purpose is found under Documents. If the investigation shows that it is not possible to replace the CMR-product, then it is required to follow documented handling instructions and to conduct written risk assessments before work starts. 

The following information should be clearly included in the risk assessment:

1. In which places and areas will the product be handled and what measures will be taken to ensure that only people who are necessary on the workplace are present in this area?
2. What safety measures will be taken to ensure that the exposure is minimal?
3. In what situations is it important with personal protective equipment?
4. Handling and functioning of the technical equipment, processes and ventilation should be monitored to detect abnormalities early that can lead to increased risk.

It is important to make a careful risk assessment and to develop routines so that the handling of CMR substances is carried out in such a way that the risk of hazardous exposure is minimized.

CMR exposure register

If there is a risk that people have been exposed to health-hazardous levels of a CMR substance or during activities defined in AFS 2011:19 § 38 second part due to e.g. accident, spillage, ventilation interruption, non-compliance with routines/instructions, failure to assess risk the employer must establish a register of the persons who have been exposed, as well as define the estimated level of exposure.

Note! The requirement to establish an exposure register also applies to work that involves skin exposure to mineral oils that have previously been used to lubricate and cool moving parts in an engine (e.g. when changing oil).

The register should contain:

• The employee's name and social security number
• Duties and the period when the work was conducted
• Substances
• Measured or estimated levels of exposure

This register must be kept at Karolinska Institutet for at least 40 years, except for products labelled with the hazard statement H360, which must be kept for at least 5 years. The aim of the register is to facilitate investigations of work-related diseases. 

The prefect or a person who got this task delegated is responsible for the registration. The form for the register and guidance can be found here (only in Swedish).

Purchase of chemical products

Purchases of new chemicals must ensure that the amount of chemicals purchased are minimized and that the chemicals are only intended for use within KI's activities. The number of people who order chemicals should be limited. Each department / equivalent should appoint one or several persons responsible for purchase, who are familiar with agreements and regulations in that field.

Purchases are made via contracted suppliers. Information on framework agreements and direct procurement can be found on the Staff Portal under Purchasing and public procurement.

Delivered packages must be stored securely until the delivery is taken over by persons from the department / equivalent with best knowledge of the type of product.

Preparations before the purchase of chemical products:

• A risk assessment should be made within the order to prevent risks that may arise anywhere during the purchase chain. The entire management, from purchasing to waste management, must be risk assessed.
• Investigate whether the chemical products require a permit or is prohibited from use. Any permits and dispensaries must be in place before order.
• For CMR classified products, a documented investigation should be made, to investigate if there is a possibility to use a less hazardous product instead.
• Some allergy-causing chemical products require training certificates before handling. The training must be finished before the order is made.
• For some products it is required that the employer arranges medical controls before work starts, e.g. lead, cadmium and mercury.

When importing / purchasing chemical products from other countries outside the EU and EEA special import regulations apply. Read the “Reach import document” (see under Documents). 

If you have any questions, please contact kemikaliesakerhet@ki.se.

Risk assessment for handling of hazardous chemicals

Before the laboratory work with chemicals begins, evaluate whether there are any risks with the handling/method, and if protective measures and handling instructions are required. There must also be an emergency routine in place in case of spill, accident, leakage, or fire.

The nearest manager is responsible for carrying out risk assessments, however, a risk assessment must be made in any case by the person who will perform the handling/method. The nearest manager reviews and approves (signs) the risk assessment.

Safety, work environment, and the external environment must be considered in the risk assessment. The requirement for a risk assessment applies to all handling, including storage, transportation, and waste management. Consideration should also be given to the premises, employees, and other staff. It is crucial to ensure that poisons, toxins, and other hazardous substances are not accessible to unauthorized individuals. Additionally, there may be risks related to sabotage and espionage.

If substances with unknown contents are handled, the precautionary principle applies, i.e., the substance must be handled as dangerous for both human and environment.

Risk assessments must be kept close to the workplace, and must be regularly reviewed, especially if new circumstances or knowledge is added.

It is important that everyone affected in the workplace is informed about and understands the risks involved. For example, other research groups working in the same laboratory should also be informed, and it may be necessary for the risk assessment to be written in both Swedish and English.

In KLARA, there is a module for risk assessment with detailed instructions. The "Risk Assessor" profile can be obtained by contacting the departmental chemicals representative. Courses/workshops in KLARA risk management are regularly offered to all KI employees. More information on the Staff portal page Courses in Safety- and security.

Routine of Risk assessment

A written risk assessment should preferably be made in the KLARA risk assessment tool or on a plain sheet of paper. To start with, make a risk assessment of the most common methods that pose the greatest risk (e.g., most hazardous chemical). Focus on the most dangerous parts (risky moments) of the overall handling.

The risk assessment should include:

• Method description and premises
• Risk sources as substances/products, incl. classification, concentration, amount, etc.
• Risky moments and situations
• Type of exposure (inhalation, skin contact, etc.)
• Personal protection (gloves, googles, etc.)
• Preventive measures (training, medical check-up, etc.)
• Actions in case of spill, accident, leakage, or fire
• Estimated total risk (should be low, otherwise you have to adapt accordingly, e.g., additional protective measures, smaller quantities, etc.)
• Name of the risk assessor (the person/persons performing the handling)
• Signature (group or research leader)

Substitution and phase-out of hazardous chemicals

By law, hazardous chemicals must be phased out or their use reduced / minimized. This applies to products consisting of or containing:

• Halogenated Solvents (replaced with Non-halogenated)
• Lead, cadmium, mercury and other heavy metals
• CMRs (carcinogenic, mutagenic and / or reproductive toxic)

KI:s laboratories must continuously work actively to reduce the amount of these substances. 

Practically, substitution involves investigation whether there are alternatives to the hazardous chemicals used in teaching and research, for example by:

• replace a single chemical (e.g. a solvent)
• change to another method where less dangerous products are use

If it is not possible to substitute, one should instead try to minimize the amount of dangerous chemicals used, e.g. by:

• make fewer experiments and / or work on a smaller scale
• discard old chemicals and unused ones
• order smaller quantities when purchasing new products 
• buy kits and ready-made mixes instead of clean and concentrated products

More information about substitution, see Links below.

Chemical goal for 2017-2019: KI’s reduction list 

As part of KI’s general environmental, sustainability and work-environment efforts, a list of eleven chemicals has been compiled for KI:s laboratories to consult when prioritizing which chemicals to reduce, until 2019.

• Triton X-100
• Acrylamide
• Formaldehyde
• Blue gel
• Boric acid
• Dimetylformamide (DMF)
• Imidazole
• Hydrazine compound
• Cobolt cloride
• Nickel cloride
• Ethidium bromide

Products containing one or more of these substances are listed on the "KI reduction list" in the KLARA product database. These eleven substances are selected on the basis that they are CMR classified and / or environmentally hazardous and they are used in large quantities at Karolinska Institutet.

The chemical goal for 2017 - 2019 is now formally completed. The final report can be found here (only in Swedish).

Information about KI’s reduction list in KLARA will remain so that KI’s laboratories will continue to replace or reduce these substances even after the chemical goal 2017 -2019 has been completed.

National substitution group (NSG)

Karolinska Institutet is active in NSG, which is a national network with the aim of jointly helping to exchange hazardous chemicals in health care (including dental care) and research. By exchanging experience on completed substitutions, each company's substitution work can be facilitated.

NSG has produced a Substitution list for hazardous chemicals with some examples that everyone can use. More information here.

If you have made substitutions yourself, it is important to notify NSG via kemikaliesakerhet@ki.se.

Chemicals subject to authorization

There are chemicals that may not be handled and there are chemicals that require a permission or a dispensation to be handled. It is the responsible manager/head of department who is responsible for permits and that they are valid. Permits are applied for by the individual research group by contacting the responsible coordinator at the Safety and security unit via kemikaliesakerhet@ki.se.

The coordinator will help you with which forms to fill in, to send the completed forms to the responsible authority and to record necessary documents in the KI register. Chemicals may only be used as described in the corresponding permit.

It is not allowed to lend or hand over chemicals that are subject to authorization. The permit states which rules apply regarding handling, for example how the chemical should be used, stored and in which premises it may be used. Different licensing authorities have different requirements, but common requirements are risk assessment, handling instructions and a emergency procedures in place.

Permits are time-limited and it is important that there is a procedure within the department to keep track of when it is time to renew a permit. In KLARA there is a function called Permit which is a helpful tool for this (can be found under Additional functions at the registry for each research group).

If substances subject to authorization are handled without a permit/dispensation, the department, in some cases, risks a fine of several hundred thousand SEK. Substances requiring authorization are:

• Mercury and mercury compounds (Swedish Chemicals Agency)
• Group A substances (Swedish Work Environment Authority)
• Group B substances (Swedish Work Environment Authority)
• Goods dangerous to health (Public Health Agency of Sweden)
• Narcotic precursors (Swedish Medical Products Agency)

In KLARA there are reference lists to be used for self-monitoring of the above regulated substances (the one for narcotic precursors is called Medical Products Agency Category 1). Further rules regarding handling of narcotic precursors can be found on the web page Narcotic drugs and narcotic drug precursors.

An updated list of substances covered by the requirements for permit concerning goods dangerous to health can be found in the annex to SFS 1999:58 (regulation on the prohibition of certain goods dangerous to health, in Swedish), see link further down the page under Links.

It is not only pure substances that require a permit. Also, chemical products or kits containing small quantities of an authorized chemical, can be subject to a permit. Products containing 0.1% by weight or more of a Group A substance require authorization and for Group B substances the limit is 1% by weight.

Substances listed in Annex XIV to the REACH Regulation do not require permits for use in scientific research and development. This includes use in analysis activities, provided that the substance is used under controlled conditions and not in quantities larger than 1 ton per year. Substances that are particularly hazardous and which, according to proposals, may end up in Annex XIV are on the so-called candidate list.

Ozone-depleting substances - restrictions in use

Ozone depleting substances (ODS) are chemicals that destroy the earth’s protective ozone layer. Therefore, it is very important to minimize the use of ODS as soon as there are adequate alternatives. EU regulation 2024/590 regulates the use of ODS and in appendix 1 the controlled substances are listed. Some examples of substances covered are carbon tetrachloride (tetrachloromethane) and 1-bromo-2-fluoroethane.

Before purchasing an ODS, you must investigate whether the use is permitted or not. It is only permitted if there is no technically and/or economically feasible alternative or where the alternative is less acceptable for the environment and health. Permitted and prohibited uses are stated in the annex to EU regulation 291/2011.

The person responsible for operations where such a substance is handled must ensure that there is a routine for keeping records. For each substance, you need to register the name, quantity used, the purpose of use and a list of suppliers. The register needs to be kept for at least five years.

If you have any questions, contact kemikaliesakerhet@ki.se

Storage of chemicals

Chemical products should be stored in a safe manner to avoid accident, health risks, and release to the environment. The head of department or equivalent is utmost responsible for chemical products being stored in a proper manner. Storage conditions should be based on the properties of the chemical products and guidance is often given in the product’s SDS.

General storage conditions are:

• All storage places where chemicals are stored must be clearly marked with relevant pictogram.
• Chemical products should be stored in ventilated cabinets or storages.
• Chemical products shall not be stored so that there is a risk that they accidentally end up in the sewage (e.g. in a fume hood without embankment, on shelves above a sink)
• Acids shall be stored in acid-resistant and ventilated cabinets - and never together with bases or organic substances.
• Corrosive chemicals (acids and bases) should be stored below waist height.
• Oxidizing agents should not be stored with oxidizable substances.
• Flammable products must be stored in fireproof cabinets.
• Flammable products should not be stored with easily combustible substances/materials or with gases.
• For flammable products that require cool storage, use EX-classified refrigerators/freezers intended for this purpose (not the usual household fridges/freezers).
• Gas cylinders should be stored well anchored with a chain or similar.
• Flammable gas must be stored fire-proof.
• Peroxide-forming chemicals should be stored in a dark and cool place. A special handling routine is required.
• Chemical products that are serious health hazards/acute toxics (poisons and CMR substances) must be stored in locked poison boxes.
• A- and B-classified chemical products shall be stored according to the unique permit conditions.

Look at the product's label, i.e. which hazard pictograms (CLP) are on the package for guidance on how to store it.

Exception! Only products with a flash point of up to 60 degrees are classified according to CLP as flammable, i.e. they are marked with pictograms. But according to MSB's classification for flammable products, all products that have a flash point between 60 degrees and 100 degrees are also considered flammable, but they are not classified according to CLP, i.e. not marked with a pictogram. These products must also be stored in fireproof cabinets.

To find more information about the chemical properties of the product, you can read the product's safety data sheet (SDS) under e.g. section 7 "Handling and Storage" and section 10 "Stability and Reactivity".

Liquid nitrogen

Liquid nitrogen is a colorless, odorless and tasteless liquid. The gas is non-toxic and does not burn, but there is a risk of the oxygen in the air being displaced - which can cause oxygen deficiency. Liquid nitrogen can give cold-burns because of the low boiling point of -196 °C.

Work involving liquid nitrogen may only be carried out by those who have adequate knowledge of the chemical and the potential risks that handling and use entail, together with how these risks can be avoided. The nearest manager should ensure that:

• a written risk assessment is carried out before work involving liquid nitrogen begins
• adequate protective measures have been taken
• local handling and protection instructions have been developed

The nearest manager should also ensure that employees who handle liquid nitrogen are aware of the risks and have been informed about local handling and safety instructions. More information see KI:s Rules for handling liquid nitrogen under Documents.

Dry ice

Dry ice is solid carbon dioxide which changes directly from solid into gas at a temperature of -78 °C and higher.

Approximately 530 liters of carbon dioxide gas are formed from one kilogram of dry ice during evaporation. The container for dry ice should therefore never be completely closed to prevent excessive pressure build-up.

The main hazards of dry ice include severe frost bite due to the temperature of -78°C and suffocation due to excessive concentration of carbon dioxide during evaporation, as it displaces the oxygen.

The rate of evaporation depends on the quantity of dry ice, its packing, and storage.
Some examples of the evaporation rate (Linde AG):

• 1 kg at room temperature on a table, 6–8 hours

• 10 kg at room temperature in a Styrofoam box, 1–2 days

• 20 kg at room temperature in a Styrofoam box, 3–4 days

• 20 kg in a freezer in a Styrofoam box, 4–5 days.

Dry ice is manufactured in the form of pellets with a size of 10 mm in diameter and a length of approximately 20 mm or as 1 kg dry ice blocks (125x27x210mm).

The best storage container for dry ice is a Styrofoam box or cooler bag. KI advises against the use of thermos containers due to incidents where thermoses have imploded.

Technical alcohol

Must be continuously documented:

• Details of the amount of alcohol you bought and consumed (does not apply to fully denatured ethanol).

To be inventoried:

• Your stock of technical alcohol at least once per calendar year.

• If there is a difference between the inventoried quantity and the expected stock quantity, this must be investigated, and the necessary measures taken.

For technical spirit to be considered fully denatured, it must contain all the components below.

Per hectolitre (100l) of absolute ethanol: 

• 1 litre of isopropyl alcohol
• 2 litres of methyl ethyl ketone
• 1 gram of denatonium benzoate.

Most products handled at KI contain only one of the components and are therefore NOT fully denatured.

More information is available on the Public Health Agency's website under Technical spirits and alcoholic preparations.

Your department has local procedures for how this should be handled.

Formaldehyde

Formaldehyde is classified as H350 (may cause cancer), and therefore subject to "Special requirements for carcinogenic, mutagenic and toxic to reproduction chemicals and certain activities" (38 § - 44 §) in AFS 2011: 19. 

One requirement is that a documented investigation must be conducted to find out if it is possible to use a less harmful product instead.

Formaldehyde is also classified as H317 (may cause an allergic skin reaction) and is therefore covered by "Special requirements for allergenic chemicals and some processes" (37 a § - 37 g §) in AFS 2011: 19, e.g. requirements regarding risk assessment, signs and information.

Before handling of formaldehyde starts it is important to follow handling instructions and to conduct risk assessments. The result of the risk assessment should show that there is no risk of dangerous exposure in the handling. Examples of actions to take to limit the exposure is to work in specially adapted laboratories, to handle formaldehyde in a ventilated workplace (fume hood) and to use the right kind of laboratory gloves (standards and materials are often specified in the product’s safety data sheets).

All staff at the workplace must be informed that formaldehyde is handled in the premises and how to protect against dangerous exposure.

If there are pregnant and nursing employees in the workplace it is crucial to perform a special risk assessment which takes this into account.

Formaldehyde handling during the fixation of tissues and similar does not require any training (training requirements, § 37e in AFS 2011:19).

Ethidium bromide

Ethidium bromide is a substance used in electrophoresis for DNA analysis. It binds to DNA and illuminates under UV light and allows for the DNA in a sample to be detected.

The ability to bind to DNA means that it can be hazardous to persons handling the substance, as it also binds to DNA in the human body and may cause heritable genetic damage (mutagenicity class 1 or 2).

Ethidium bromide should as far as possible be phased out.

Exposure

The content of ethidium bromide in the gels and solutions that are used are considered to be so low that the composite products are not classified as mutagenic according to the requirements of the Swedish authorities. Despite this, you should deal gently with ethidium bromide as the level of exposure not only depends on the amount and concentration, but also on how often you use the chemical and the procedures that exist in the workplace.

Laboratory and service personnel may be exposed to many different substances and the additive effect of multiple exposures is almost always unknown, which should be respected.
Ethidium bromide may be absorbed through the skin and nitrile gloves are considered to be a good protection against ethidium bromide.

See also Alternative to Ethidium Bromide under Documents.

Cytostatic drugs

Cytostatic drugs belong to the group of "Especially dangerous drugs" and the use of cytostatic drugs is regulated by the Swedish Working Environment Authority in “AFS 2005:5”. Long-term or occasionally extremely high, exposure may pose an increased risk of genetic damage and cancer.

For pregnant persons the unborn child may be affected, as well as children during lactation period.

Routines

Cytostatic drugs are a group of substances with different properties and character acting with different mechanisms. Consequently, the degree of harmful exposure is very diverse, as are the symptoms.

When working with cytostatic drugs it is important to limit exposure by:

• Conduct written risk assessments before work with cytostatic drugs starts.
• Written handling, safety and disposal instructions appropriate for local prerequisites.
• Ensuring that the person doing the work with cytostatic drugs has the right skills (responsible is the manager / head of department).
• Provide training and courses (by the manager / head of department).
• Inform other local staff (for example cleaning personnel) about the nature of the activity and how to protect themselves.

Exposure and action

Contact with skin usually gives local irritations and allergic reactions and some drugs can also be absorbed through the skin and affect whole organ system. In the long term, exposure to cytostatic drugs can cause cancer.
Any deviations, incidents and accidents associated with cytostatic drugs should immediately be reported in writing to the responsible manager.

Peroxide forming chemicals

To avoid the risk of explosion, it is important to have a procedure for handling, storage and control of peroxide-forming chemicals. These are chemicals that are classified with hazard statement EUH019.

Examples of such chemicals that are common at KI are:

Diethyl ether                       CAS: 60-29-7

1,2-Dimethoxyethane         CAS: 110-71-4

1,4-Dioxane                        CAS: 123-91-1

Tetrahydrofuran                  CAS: 109-99-9

KI's Instructions for handling chemicals that can form explosive peroxides in their entirety can be found under Documents.

Peroxide forming

Peroxide forming chemicals may over time form explosive peroxides, and they are in most cases flammable and explosive. At high levels of peroxides, it may be enough to shock or heat the container to ignite an explosion - and the higher the concentration of peroxides, the greater the risk of explosion.

Parameters that have an impact on peroxide formation are:

• Whether the bottle is opened (oxygen accelerates the process).
• How old the chemical is and how long it has been opened.
• If it is exposed to sunlight and/or heat (accelerates the formation of peroxides).
• If the product is distilled (stabilizers are "purified" off).

Prevention

• Buy smaller amounts at a time.
• Always label the product with the delivery date and the date when the seal has been broken.
• Opened products should not be stored more than one year.
• Keep the chemical in a dark and cool place.
• For liquids: Make regular checks of the peroxide content (see section Peroxide test).
• For solids: Must be discarded before the expiry date (see waste management).

Peroxide test

Note! The test may only be performed by employees who are aware of the risk in handling peroxides!

It is recommended to use strips for peroxide tests, e.g., Sigma-Aldrich part number 1.10081 (0.5-100 mg/L H2O2, MQuant; Supelco).

Chemicals with a peroxide content ≥3mg/l are not allowed to use; they must be discarded!

Waste management

You should not personally handle solutions with a peroxide content ≥3mg/l.

For solids and liquids with lower peroxide concentration (<30 mg/L):

• Contact Stena for collection directly from the storage location.

For old peroxide-containing chemicals:

• When the contents have evaporated and there is no liquid remaining, except precipitation or crystals.
• When the liquid is turbid (cloudy) or discoloured and/or there is precipitation or crystals on the bottom of the container.
• When you cannot see through the container, and you know that it has been stored unsuitably (warm) or for a long period (more than 1 year).
• When you do not know how old the container is or how it has been stored.
• When you have detected a high peroxide concentration, ≥30 mg/L.
• Contact the Safety advisor for consultation and the safety unit (kemikaliesakerhet@ki.se) for information.

Note! If a solution is turbid, discoloured or when the contents have evaporated and show signs of precipitation/crystal formation, the container should not be touched/moved. Even in cases of major peroxide formation, the chemical is not dangerous unless disturbed.

Contact information can be found on KI’s Staff Portal under Laboratory waste and Contact. 

Perchloric acid

General
Perchloric acid is a potentially dangerous substance with oxidizing, corrosive and toxic properties and can cause fire or explosion in contact with several different substances, especially organic ones.

Handling
Perchloric acid should be handled in specially ventilated fume hoods with e.g., flushable ventilation ducts that are not used for anything else. There are several criteria in MSB's general advice SÄI 1983:1 on fume hood equipment for work with perchloric acid (only in Swedish).

Recommendation, if possible:

• Replace the substance with another less dangerous substance.
• Use another method.
• If you have to continue working with perchloric acid, use a lower concentration (preferably ≤ 20%).

Note! If you are planning to work with perchloric acid and you do not have access to a specially ventilated fume hood according to MSB's regulations, contact kemikaliesakerhet@ki.se.

Protective measures

The handling of chemical products must always be preceded by a written risk assessment, which must indicate what possible protective measures must be taken to limit exposure / risks in handling. Examples of protective measures are gloves for personal protection, safety ventilation and safety goggles.

Precautionary measures are described in the chemicals Safety Data Sheet (SDS). Chemical products SDS’s must always be available for all concerned and close to the workplace where the chemicals are handled (in KLARA).

As a standard, laboratory coats should be used in the laboratory and should be removed when leaving the lab. The coat should not be made of flammable synthetic material, it should preferably have push buttons (so that it can easily be taken off in the event of fire or spillage) and have cushions. It should be washed as often as required and the laundry should be done by the employer (the coat should not be washed at home).

Safety goggles should always be used when handling corrosive chemicals, e.g. acids, bases and other substances that can damage the eyes. Lenses should preferably not be used in labs as they can burn on the eye lens and lead to blindness in the event that corrosive substances get into the eye.

Protective gloves for working with chemicals

Protective gloves protect the skin from local damage, e.g. irritation, corrosion and poisoning, which can occur as a result of chemicals being absorbed through the skin. The type of glove that protects you depends on the properties of the chemicals and how the handling should go. Information on which glove material is suitable can be found in the safety data sheet and which type e.g. the thickness is available from the supplier.

More detailed information on protective gloves can be found here.

Protected ventilated workplaces

Safety ventilation (fume hood, suction table, safety cabinet and spot extractor) is a common protective measure when handling hazardous chemical products. Mor information on page Ventilated workplaces.

When handling particularly hazardous chemicals, e.g. CMR and allergenic chemical products, always use protective ventilation. When dealing with substances with unknown properties / risks, the precautionary principle applies, which means that the substance / product should be considered dangerous.

When handling cytostatic drugs, it is important to ensure that there is no risk of hazardous exposure. Therefore, handling should take place under controlled forms, preferably in closed systems and in specially designated premises. If the work is carried out in a safety cabinet, the cabinet must be connected to external ventilation (i.e. exhaust air connected).

Spill of chemicals

In case of hazardous chemicals spills, it is important to take action quickly so that personnel are not harmed:

• In the event of major spills, KI's central emergency procedure for larger spills and emissions of hazardous chemicals should be followed. If necessary, consult a specialist for advice (see under Documents).
• Evacuate, cordon the area and inform colleagues that a spill has occurred.
• Consult local emergency routine (for lab, house). Read the risk assessment and safety data sheet for the current chemical / method to find out more about the chemical properties.
• Clean it yourself if possible (with spill kit), always use protective equipment.

Never let the cleaning staff clean up! They do not have the skills needed. An incident must be reported in the Incident Management System as well as to the nearest manager.

NOTE! Departments located at the hospital shall apply the corresponding rules of the hospital.

Contact: